Abstract
The rate of strawberry propagation through conventional technique is quite low and it is difficult to maintain planting material during the summer months under Bihar condition. Further, importing mother plants adds to the production cost. In vitro micro propagation has emerged as a potential alternative for supplying planting material for strawberry. Two type of explants viz., runner tip and nodal segment were used for the study. Phenol exudation was the major problem during establishment which caused death of majority explants. In our experiment, almost no phenolic exudation (+) and maximum percent regeneration for runner tip (55.2 ± 0.52%) and nodal segment (58.1 ± 0.54%) was observed when MS medium was supplemented with ascorbic acid 200 mg per liter. Phenolic exudation was recorded highest (++++) under control when no antioxidants were supplemented. Minimum number of days for runner tips (8.4 ± 0.23) and nodal segments (10.3 ± 0.33) taken for shoot proliferation was observed when MS medium was supplemented with activated charcoal 300 mg and 200 mg per liter, respectively. Though all other antioxidants used in our study including citric acid, PVP and activated charcoal significantly reduced oxidative browning, ascorbic acid was found to be most effective antioxidant in controlling lethal browning during in vitro establishment of strawberry. This protocol has a potential for allowing a large scale multiplication of this important crop.
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Claims 10
Claim · model-written summary
Ascorbic acid at 200 mg per liter produced almost no phenolic exudation.
- Taxon
- strawberry
_single2.jpg?width=60)
Fragaria ×ananassat0 - Treatment
- ascorbic acid 200 mg per litere0
- Outcome
- phenolic exudatione0
- Direction
- almost noe0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_single2.jpg?width=500)
Verbatim
e0“almost no phenolic exudation (+) and maximum percent regeneration for runner tip (55.2 ± 0.52%) and nodal segment (58.1 ± 0.54%) was observed when MS medium was supplemented with ascorbic acid 200 mg per liter”
t0“Strawberry”
Claim · model-written summary
Ascorbic acid at 200 mg per liter gave maximum runner tip regeneration of 55.2 ± 0.52%.
- Taxon
- strawberryFragaria ×ananassat0
- Organ / stage
- runner tipe0
- Treatment
- ascorbic acid 200 mg per litere0
- Outcome
- percent regeneratione0
- Direction
- maximume0
- Value
- 55.2 ± 0.52%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“maximum percent regeneration for runner tip (55.2 ± 0.52%) and nodal segment (58.1 ± 0.54%) was observed when MS medium was supplemented with ascorbic acid 200 mg per liter”
t0“Strawberry”
Claim · model-written summary
Ascorbic acid at 200 mg per liter gave maximum nodal segment regeneration of 58.1 ± 0.54%.
- Taxon
- strawberryFragaria ×ananassat0
- Organ / stage
- nodal segmente0
- Treatment
- ascorbic acid 200 mg per litere0
- Outcome
- percent regeneratione0
- Direction
- maximume0
- Value
- 58.1 ± 0.54%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“maximum percent regeneration for runner tip (55.2 ± 0.52%) and nodal segment (58.1 ± 0.54%) was observed when MS medium was supplemented with ascorbic acid 200 mg per liter”
t0“Strawberry”
Claim · model-written summary
The no antioxidant control had the highest phenolic exudation.
- Taxon
- strawberryFragaria ×ananassat0
- Outcome
- Phenolic exudatione0
- Direction
- higheste0
- Comparator
- controle0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Phenolic exudation was recorded highest (++++) under control when no antioxidants were supplemented”
t0“Strawberry”
Claim · model-written summary
Activated charcoal at 300 mg per liter minimized time to runner tip shoot proliferation to 8.4 ± 0.23 days.
- Taxon
- strawberryFragaria ×ananassat0
- Organ / stage
- runner tipse0
- Treatment
- activated charcoal 300 mge0
- Outcome
- number of dayse0
- Direction
- Minimume0
- Value
- 8.4 ± 0.23e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Minimum number of days for runner tips (8.4 ± 0.23) and nodal segments (10.3 ± 0.33) taken for shoot proliferation was observed when MS medium was supplemented with activated charcoal 300 mg and 200 mg per liter, respectively”
t0“Strawberry”
Claim · model-written summary
Activated charcoal at 200 mg per liter minimized time to nodal segment shoot proliferation to 10.3 ± 0.33 days.
- Taxon
- strawberryFragaria ×ananassat0
- Organ / stage
- nodal segmentse0
- Treatment
- activated charcoale0
- Outcome
- number of dayse0
- Direction
- Minimume0
- Value
- 10.3 ± 0.33e0
- Qualifier
- 200 mg per litere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Minimum number of days for runner tips (8.4 ± 0.23) and nodal segments (10.3 ± 0.33) taken for shoot proliferation was observed when MS medium was supplemented with activated charcoal 300 mg and 200 mg per liter, respectively”
t0“Strawberry”
Claim · model-written summary
Citric acid significantly reduced oxidative browning.
- Taxon
- strawberryFragaria ×ananassat0
- Treatment
- citric acide0
- Outcome
- oxidative browninge0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“citric acid, PVP and activated charcoal significantly reduced oxidative browning”
t0“Strawberry”
Claim · model-written summary
PVP significantly reduced oxidative browning.
- Taxon
- strawberryFragaria ×ananassat0
- Treatment
- PVPe0
- Outcome
- oxidative browninge0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“citric acid, PVP and activated charcoal significantly reduced oxidative browning”
t0“Strawberry”
Claim · model-written summary
Activated charcoal significantly reduced oxidative browning.
- Taxon
- strawberryFragaria ×ananassat0
- Treatment
- activated charcoale0
- Outcome
- oxidative browninge0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“citric acid, PVP and activated charcoal significantly reduced oxidative browning”
t0“Strawberry”
Claim · model-written summary
Ascorbic acid was the most effective antioxidant against lethal browning.
- Taxon
- strawberryFragaria ×ananassae0
- Treatment
- ascorbic acide0
- Outcome
- lethal browninge0
- Direction
- most effectivee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ascorbic acid was found to be most effective antioxidant in controlling lethal browning during in vitro establishment of strawberry”
The same questions in other papers
ascorbic acid · disease · Fragaria x ananassa (strawberry)Positive and negative results
3 papers in the same direction as this paper · 1 in the opposite direction
- negative
- positive
- positive
1 paper more in this group
See all 5 papers →ascorbic acid · growth · Fragaria x ananassa (strawberry)Results agree
2 papers in the same direction as this paper
- positive
- positive
citric acid · disease · Fragaria x ananassa (strawberry)Results agree
3 papers in the same direction as this paper
- positive
- positive
- positive
ascorbic acid · quality: phytochemicals · Fragaria x ananassa (strawberry)Results agree
3 papers in the same direction as this paper
- positive
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.