Abstract
To unravel the molecular mechanisms underpinning maize (Zea mays L.) drought stress tolerance, we conducted comprehensive comparative transcriptome and physiological analyses of drought-tolerant YE8112 and drought-sensitive MO17 inbred line seedlings that had been exposed to drought treatment for seven days. Resultantly, YE8112 seedlings maintained comparatively higher leaf relative water and proline contents, greatly increased peroxidase activity, but decreased malondialdehyde content, than MO17 seedlings. Using an RNA sequencing (RNA-seq)-based approach, we identified a total of 10,612 differentially expressed genes (DEGs). From these, we mined out four critical sets of drought responsive DEGs, including 80 specific to YE8112, 5140 shared between the two lines after drought treatment (SD_TD), five DEGs of YE8112 also regulated in SD_TD, and four overlapping DEGs between the two lines. Drought-stressed YE8112 DEGs were primarily associated with nitrogen metabolism and amino-acid biosynthesis pathways, whereas MO17 DEGs were enriched in the ribosome pathway. Additionally, our physiological analyses results were consistent with the predicted RNA-seq-based findings. Furthermore, quantitative real-time polymerase chain reaction (qRT-PCR) analysis and the RNA-seq results of twenty representative DEGs were highly correlated (R2 = 98.86%). Crucially, tolerant line YE8112 drought-responsive genes were predominantly implicated in stress signal transduction; cellular redox homeostasis maintenance; MYB, NAC, WRKY, and PLATZ transcriptional factor modulated; carbohydrate synthesis and cell-wall remodeling; amino acid biosynthesis; and protein ubiquitination processes. Our findings offer insights into the molecular networks mediating maize drought stress tolerance.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 12
Claim · model-written summary
YE8112 maintained higher leaf relative water and proline contents than MO17 under seven days of drought
- Material
- YE8112e2
- Organ / stage
- seedlingse2
- Treatment
- drought treatment for seven dayse0
- Outcome
- leaf relative water and proline contentse2
- Direction
- highere2
- Comparator
- MO17e2
Zea maysOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“To unravel the molecular mechanisms underpinning maize (Zea mays L.) drought stress tolerance”
e2“YE8112 seedlings maintained comparatively higher leaf relative water and proline contents, greatly increased peroxidase activity, but decreased malondialdehyde content, than MO17 seedlings”
e0“seedlings that had been exposed to drought treatment for seven days”
Claim · model-written summary
YE8112 showed greatly increased peroxidase activity compared with MO17 under drought
- Material
- YE8112e2
- Organ / stage
- seedlingse2
- Treatment
- drought treatment for seven dayse0
- Outcome
- peroxidase activitye2
- Direction
- greatly increasede2
- Comparator
- MO17e2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“To unravel the molecular mechanisms underpinning maize (Zea mays L.) drought stress tolerance”
e2“YE8112 seedlings maintained comparatively higher leaf relative water and proline contents, greatly increased peroxidase activity, but decreased malondialdehyde content, than MO17 seedlings”
e0“seedlings that had been exposed to drought treatment for seven days”
Claim · model-written summary
YE8112 showed decreased malondialdehyde content compared with MO17 under drought
- Material
- YE8112e2
- Organ / stage
- seedlingse2
- Treatment
- drought treatment for seven dayse0
- Outcome
- malondialdehyde contente2
- Direction
- decreasede2
- Comparator
- MO17e2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“To unravel the molecular mechanisms underpinning maize (Zea mays L.) drought stress tolerance”
e2“YE8112 seedlings maintained comparatively higher leaf relative water and proline contents, greatly increased peroxidase activity, but decreased malondialdehyde content, than MO17 seedlings”
e0“seedlings that had been exposed to drought treatment for seven days”
Claim · model-written summary
RNA-seq identified 10,612 differentially expressed genes in drought-treated maize seedlings
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“To unravel the molecular mechanisms underpinning maize (Zea mays L.) drought stress tolerance”
e3“we identified a total of 10,612 differentially expressed genes (DEGs)”
Claim · model-written summary
Eighty drought-responsive DEGs were specific to YE8112
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“To unravel the molecular mechanisms underpinning maize (Zea mays L.) drought stress tolerance”
e4“we mined out four critical sets of drought responsive DEGs, including 80 specific to YE8112, 5140 shared between the two lines after drought treatment (SD_TD), five DEGs of YE8112 also regulated in SD_TD, and four overlapping DEGs between the two lines”
Claim · model-written summary
Five thousand one hundred forty drought-responsive DEGs were shared between YE8112 and MO17 after drought
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“To unravel the molecular mechanisms underpinning maize (Zea mays L.) drought stress tolerance”
e4“we mined out four critical sets of drought responsive DEGs, including 80 specific to YE8112, 5140 shared between the two lines after drought treatment (SD_TD), five DEGs of YE8112 also regulated in SD_TD, and four overlapping DEGs between the two lines”
Claim · model-written summary
Five DEGs of YE8112 were also regulated in the shared drought set
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“To unravel the molecular mechanisms underpinning maize (Zea mays L.) drought stress tolerance”
e4“we mined out four critical sets of drought responsive DEGs, including 80 specific to YE8112, 5140 shared between the two lines after drought treatment (SD_TD), five DEGs of YE8112 also regulated in SD_TD, and four overlapping DEGs between the two lines”
Claim · model-written summary
Four overlapping DEGs were found between the two inbred lines
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“To unravel the molecular mechanisms underpinning maize (Zea mays L.) drought stress tolerance”
e4“we mined out four critical sets of drought responsive DEGs, including 80 specific to YE8112, 5140 shared between the two lines after drought treatment (SD_TD), five DEGs of YE8112 also regulated in SD_TD, and four overlapping DEGs between the two lines”
Claim · model-written summary
Drought-stressed YE8112 DEGs were primarily associated with nitrogen metabolism and amino-acid biosynthesis pathways
- Material
- YE8112e5
- Treatment
- Drought-stressede5
- Outcome
- nitrogen metabolism and amino-acid biosynthesis pathwayse5
- Direction
- primarily associated withe5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“To unravel the molecular mechanisms underpinning maize (Zea mays L.) drought stress tolerance”
e5“Drought-stressed YE8112 DEGs were primarily associated with nitrogen metabolism and amino-acid biosynthesis pathways, whereas MO17 DEGs were enriched in the ribosome pathway”
Claim · model-written summary
MO17 DEGs were enriched in the ribosome pathway under drought
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“To unravel the molecular mechanisms underpinning maize (Zea mays L.) drought stress tolerance”
e5“Drought-stressed YE8112 DEGs were primarily associated with nitrogen metabolism and amino-acid biosynthesis pathways, whereas MO17 DEGs were enriched in the ribosome pathway”
Claim · model-written summary
qRT-PCR and RNA-seq results for twenty representative DEGs were highly correlated
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“To unravel the molecular mechanisms underpinning maize (Zea mays L.) drought stress tolerance”
e6“quantitative real-time polymerase chain reaction (qRT-PCR) analysis and the RNA-seq results of twenty representative DEGs were highly correlated (R2 = 98.86%)”
Claim · model-written summary
Drought-responsive genes of tolerant line YE8112 were predominantly implicated in stress signal transduction
- Material
- YE8112e7
- Outcome
- drought-responsive genese7
- Qualifier
- predominantly implicated in stress signal transductione7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“To unravel the molecular mechanisms underpinning maize (Zea mays L.) drought stress tolerance”
e7“tolerant line YE8112 drought-responsive genes were predominantly implicated in stress signal transduction”
The same questions in other papers
drought · physiology: osmolytes · Zea mays (maize)Results differ
27 papers in the same direction as this paper · 16 in the opposite direction
- positive
- positive
- negative
40 papers more in this group
See all 44 papers →drought · physiology: antioxidant enzymes · Zea mays (maize)Results differ
31 papers in the same direction as this paper · 28 in the opposite direction
- positive
- negative
- negative
56 papers more in this group
See all 60 papers →drought · physiology: oxidative · Zea mays (maize)Positive and negative results
7 papers in the same direction as this paper · 88 in the opposite direction
- positive
- negative
- negative
92 papers more in this group
See all 96 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.