Abstract
Since the propagation of plant viruses depends on various host susceptibility factors, deficiency in them can prevent viral infection in cultivated and model plants. Recently, we identified the susceptibility factor Essential for poteXvirus Accumulation 1 (EXA1) in Arabidopsis thaliana, and revealed that EXA1-mediated resistance was effective against three potexviruses. Although EXA1 homolog genes are found in tomato and rice, little is known about which viruses depend on EXA1 for their infection capability and whether the function of EXA1 homologs in viral infection is conserved across multiple plant species, including crops. To address these questions, we generated knockdown mutants using virus-induced gene silencing in two Solanaceae species, Nicotiana benthamiana and tomato. In N. benthamiana, silencing of an EXA1 homolog significantly compromised the accumulation of potexviruses and a lolavirus, a close relative of potexviruses, whereas transient expression of EXA1 homologs from tomato and rice complemented viral infection. EXA1 dependency for potexviral infection was also conserved in tomato. These results indicate that EXA1 is necessary for effective accumulation of potexviruses and a lolavirus, and that the function of EXA1 in viral infection is conserved among diverse plant species.
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Claims 5
Claim · model-written summary
Silencing an EXA1 homolog significantly reduced potexvirus accumulation in N. benthamiana.
- Taxon
- N. benthamiana
Nicotiana benthamianae0 - Treatment
- silencing of an EXA1 homologe0
- Interactor
- potexviruses
Potexviruse0pathogen - Gene
- EXA1 homologe0
- Outcome
- accumulation of potexvirusese0
- Direction
- compromisede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In N. benthamiana, silencing of an EXA1 homolog significantly compromised the accumulation of potexviruses and a lolavirus”
Claim · model-written summary
Silencing an EXA1 homolog significantly reduced lolavirus accumulation in N. benthamiana.
- Taxon
- N. benthamianaNicotiana benthamianae0
- Treatment
- silencing of an EXA1 homologe0
- Interactor
- a lolaviruse0pathogen
- Gene
- EXA1 homologe0
- Outcome
- accumulation of potexviruses and a lolaviruse0
- Direction
- compromisede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In N. benthamiana, silencing of an EXA1 homolog significantly compromised the accumulation of potexviruses and a lolavirus”
Claim · model-written summary
Transient expression of tomato EXA1 homologs complemented viral infection in N. benthamiana.
- Taxon
- N. benthamianaNicotiana benthamianae0
- Treatment
- transient expression of EXA1 homologs from tomatoe0
- Gene
- EXA1 homologs from tomatoe0
- Outcome
- viral infectione0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In N. benthamiana, silencing of an EXA1 homolog significantly compromised the accumulation of potexviruses and a lolavirus, a close relative of potexviruses, whereas transient expression of EXA1 homologs from tomato and rice complemented viral infection”
Claim · model-written summary
Transient expression of rice EXA1 homologs complemented viral infection in N. benthamiana.
- Taxon
- N. benthamianaNicotiana benthamianae0
- Treatment
- transient expression of EXA1 homologs from tomato and ricee0
- Gene
- EXA1 homologs from tomato and ricee0
- Outcome
- viral infectione0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In N. benthamiana, silencing of an EXA1 homolog significantly compromised the accumulation of potexviruses and a lolavirus, a close relative of potexviruses, whereas transient expression of EXA1 homologs from tomato and rice complemented viral infection”
Claim · model-written summary
EXA1 dependence of potexvirus infection was conserved in tomato.
- Taxon
- tomato
Solanum lycopersicume0 - Interactor
- potexvirale0pathogen
- Gene
- EXA1e0
- Outcome
- EXA1 dependency for potexviral infectione0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“EXA1 dependency for potexviral infection was also conserved in tomato”
The same questions in other papers
loss of function · molecular: metabolites · Nicotiana benthamianaPositive and negative results
9 papers in the same direction as this paper · 25 in the opposite direction
- negative
- negative
- no effect
31 papers more in this group
See all 35 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.