Abstract
Recognition of microbe-associated molecular patterns (MAMPs) derived from invading pathogens by plant pattern recognition receptors (PRRs) initiates a subset of defense responses known as pattern-triggered immunity (PTI). Transcription factors (TFs) orchestrate the onset of PTI through complex signaling networks. Here, we characterized the function of ERF19, a member of the Arabidopsis thaliana ethylene response factor (ERF) family. ERF19 was found to act as a negative regulator of PTI against Botrytis cinerea and Pseudomonas syringae. Notably, overexpression of ERF19 increased plant susceptibility to these pathogens and repressed MAMP-induced PTI outputs. In contrast, expression of the chimeric dominant repressor ERF19-SRDX boosted PTI activation, conferred increased resistance to the fungus B. cinerea, and enhanced elf18-triggered immunity against bacteria. Consistent with a negative role for ERF19 in PTI, MAMP-mediated growth inhibition was weakened or augmented in lines overexpressing ERF19 or expressing ERF19-SRDX, respectively. Using biochemical and genetic approaches, we show that the transcriptional co-repressor Novel INteractor of JAZ (NINJA) associates with and represses the function of ERF19. Our work reveals ERF19 as a novel player in the mitigation of PTI, and highlights a potential role for NINJA in fine-tuning ERF19-mediated regulation of Arabidopsis innate immunity.
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Claims 9
Claim · model-written summary
ERF19 negatively regulated PTI against Botrytis cinerea and Pseudomonas syringae.
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianae1 - Interactor
- Botrytis cinerea
Botrytis cinereae0pathogen - Second interactor
- Pseudomonas syringae
Pseudomonas syringaee0pathogen - Gene
- ERF19e0
- Outcome
- PTIe0
- Direction
- negativee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ERF19 was found to act as a negative regulator of PTI against Botrytis cinerea and Pseudomonas syringae”
t0“Arabidopsis”
e1“Arabidopsis thaliana”
Claim · model-written summary
ERF19 overexpression increased susceptibility to the pathogens.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Treatment
- overexpression of ERF19e0
- Gene
- ERF19e0
- Outcome
- plant susceptibilitye0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“overexpression of ERF19 increased plant susceptibility to these pathogens”
t0“Arabidopsis”
e1“Arabidopsis thaliana”
Claim · model-written summary
ERF19 overexpression repressed MAMP-induced PTI outputs.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Treatment
- overexpression of ERF19e0
- Gene
- ERF19e0
- Outcome
- MAMP-induced PTI outputse0
- Direction
- repressede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“overexpression of ERF19 increased plant susceptibility to these pathogens and repressed MAMP-induced PTI outputs”
t0“Arabidopsis”
e1“Arabidopsis thaliana”
Claim · model-written summary
ERF19-SRDX expression boosted PTI activation.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Treatment
- expression of the chimeric dominant repressor ERF19-SRDXe0
- Gene
- ERF19-SRDXe0
- Outcome
- PTI activatione0
- Direction
- boostede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“expression of the chimeric dominant repressor ERF19-SRDX boosted PTI activation”
t0“Arabidopsis”
e1“Arabidopsis thaliana”
Claim · model-written summary
ERF19-SRDX expression increased resistance to B. cinerea.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Treatment
- expression of the chimeric dominant repressor ERF19-SRDXe0
- Interactor
- B. cinereaBotrytis cinereae0pathogen
- Gene
- ERF19-SRDXe0
- Outcome
- resistancee0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“expression of the chimeric dominant repressor ERF19-SRDX boosted PTI activation, conferred increased resistance to the fungus B. cinerea”
t0“Arabidopsis”
e1“Arabidopsis thaliana”
Claim · model-written summary
ERF19-SRDX expression enhanced elf18-triggered immunity against bacteria.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Treatment
- expression of the chimeric dominant repressor ERF19-SRDXe0
- Gene
- ERF19-SRDXe0
- Outcome
- elf18-triggered immunitye0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“expression of the chimeric dominant repressor ERF19-SRDX boosted PTI activation, conferred increased resistance to the fungus B. cinerea, and enhanced elf18-triggered immunity against bacteria”
t0“Arabidopsis”
e1“Arabidopsis thaliana”
Claim · model-written summary
ERF19 overexpression weakened MAMP-mediated growth inhibition.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Treatment
- overexpressing ERF19e0
- Gene
- ERF19e0
- Outcome
- MAMP-mediated growth inhibitione0
- Direction
- weakenede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MAMP-mediated growth inhibition was weakened or augmented in lines overexpressing ERF19 or expressing ERF19-SRDX, respectively”
t0“Arabidopsis”
e1“Arabidopsis thaliana”
Claim · model-written summary
ERF19-SRDX expression augmented MAMP-mediated growth inhibition.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Treatment
- expressing ERF19-SRDXe0
- Gene
- ERF19-SRDXe0
- Outcome
- MAMP-mediated growth inhibitione0
- Direction
- augmentede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MAMP-mediated growth inhibition was weakened or augmented in lines overexpressing ERF19 or expressing ERF19-SRDX, respectively”
t0“Arabidopsis”
e1“Arabidopsis thaliana”
Claim · model-written summary
NINJA associates with ERF19 and represses its function.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Gene
- Novel INteractor of JAZ (NINJA)e0
- Outcome
- function of ERF19e0
- Direction
- repressese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the transcriptional co-repressor Novel INteractor of JAZ (NINJA) associates with and represses the function of ERF19”
t0“Arabidopsis”
e1“Arabidopsis thaliana”
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