Abstract
Satellites associated with begomoviruses are causing huge crop losses throughout the World, particularly in South Asia. Although much is known about the diversity of begomoviruses and their satellites infecting major crops such as cotton, tomato and chilies, other hosts such as weeds and ornamental plants are not well studied. During 2006-2007, ornamental rose plants (Rosa chinensis) were found with highly stunted growth and upward leaf curling in Faisalabad, Pakistan. Another commonly growing weed (Eclipta prostrata) showing vein yellowing was also analyzed for begomovirus infection. By using the rolling circle amplification method (which does not require prior sequence information for amplifying circular single stranded DNA molecules) followed by complete nucleotide sequencing, we discovered two new begomoviruses and their satellites infecting these hosts. In line with taxonomic conventions for begomovirus species, new names Rose leaf curl virus (RoLCuV) and Eclipta yellow vein virus (EcYVV) are proposed. Phylogenetic analysis based on their complete nucleotide sequences suggested that both viruses could be clustered with already identified monopartite begomoviruses from the region. EcYVV is closely related to Cotton leaf curl Bangalore virus (84%) and RoLCuV shows close identity (83%) with Tomato leaf curl Pakistan virus. One betasatellite isolated from these hosts showed less than 78% identity with other known betasatellites and can therefore be considered as a member of a new species. The second betasatellite isolated with EcYVV was found to be an isolate of the species Kenaf leaf curl betasatellite. The infectious molecules for each of the virus/satellite were prepared and inoculated through Agrobacterium to model host Nicotiana benthamiana plants, which induced severe vein yellowing and leaf curling. We conducted further experiments to understand the flexibility of betasatellite trans-replication in the presence of non-cognate begomoviruses. The betasatellites identified in this study are promiscuous in their ability to be trans-replicated by a non-cognate, already known bipartite virus; Tomato leaf curl New Delhi virus. The potential threat and replication flexibility of replication of the different molecules in the begomovirus disease complexes studied here is discussed. Note: This thesis is under a author requested embargo until 2209, after which it will become available in accordance with end of expected copyright protection. This embargo year is intentional and not an error.
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Claims 10
Claim · model-written summary
Rose plants showed highly stunted growth and upward leaf curling in Faisalabad, Pakistan.
- Taxon
- Rosa chinensis
Rosa chinensise0 - Outcome
- stunted growth and upward leaf curlinge0
- Setting
- Faisalabad, Pakistane0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“During 2006-2007, ornamental rose plants (Rosa chinensis) were found with highly stunted growth and upward leaf curling in Faisalabad, Pakistan”
Claim · model-written summary
Eclipta prostrata showed vein yellowing.
- Taxon
- Eclipta prostrata
Eclipta prostratae1 - Outcome
- vein yellowinge1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Another commonly growing weed (Eclipta prostrata) showing vein yellowing was also analyzed for begomovirus infection”
Claim · model-written summary
A new begomovirus, Rose leaf curl virus, was identified infecting Rosa chinensis.
- Taxon
- Rosa chinensisRosa chinensise0
- Interactor
- Rose leaf curl virusRose leaf curl viruse3pathogen
- Outcome
- new begomovirusese2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“During 2006-2007, ornamental rose plants (Rosa chinensis) were found with highly stunted growth and upward leaf curling in Faisalabad, Pakistan”
e2“we discovered two new begomoviruses and their satellites infecting these hosts”
e3“new names Rose leaf curl virus (RoLCuV) and Eclipta yellow vein virus (EcYVV) are proposed”
Claim · model-written summary
A new begomovirus, Eclipta yellow vein virus, was identified infecting Eclipta prostrata.
- Taxon
- Eclipta prostrataEclipta prostratae1
- Interactor
- Eclipta yellow vein virusEclipta yellow vein viruse3pathogen
- Outcome
- new begomovirusese2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Another commonly growing weed (Eclipta prostrata) showing vein yellowing was also analyzed for begomovirus infection”
e2“we discovered two new begomoviruses and their satellites infecting these hosts”
e3“new names Rose leaf curl virus (RoLCuV) and Eclipta yellow vein virus (EcYVV) are proposed”
Claim · model-written summary
Eclipta yellow vein virus is closely related to Cotton leaf curl Bangalore virus, with 84% identity.
- Taxon
- Eclipta prostrataEclipta prostratae1
- Interactor
- Eclipta yellow vein virusEclipta yellow vein viruse3pathogen
- Outcome
- closely related toe4
- Value
- 84%e4
- Comparator
- Cotton leaf curl Bangalore viruse4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Another commonly growing weed (Eclipta prostrata) showing vein yellowing was also analyzed for begomovirus infection”
e3“new names Rose leaf curl virus (RoLCuV) and Eclipta yellow vein virus (EcYVV) are proposed”
e4“EcYVV is closely related to Cotton leaf curl Bangalore virus (84%) and RoLCuV shows close identity (83%) with Tomato leaf curl Pakistan virus”
Claim · model-written summary
Rose leaf curl virus shows close identity, 83%, with Tomato leaf curl Pakistan virus.
- Taxon
- Rosa chinensisRosa chinensise0
- Interactor
- Rose leaf curl virusRose leaf curl viruse3pathogen
- Outcome
- close identitye4
- Value
- 83%e4
- Comparator
- Tomato leaf curl Pakistan viruse4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“During 2006-2007, ornamental rose plants (Rosa chinensis) were found with highly stunted growth and upward leaf curling in Faisalabad, Pakistan”
e3“new names Rose leaf curl virus (RoLCuV) and Eclipta yellow vein virus (EcYVV) are proposed”
e4“EcYVV is closely related to Cotton leaf curl Bangalore virus (84%) and RoLCuV shows close identity (83%) with Tomato leaf curl Pakistan virus”
Claim · model-written summary
A betasatellite from these hosts showed less than 78% identity with other known betasatellites and may be a new species.
- Taxon
- Rosa chinensisRosa chinensise0
- Interactor
- betasatelliteBetasatellitee5other
- Outcome
- identitye5
- Value
- less than 78%e5
- Qualifier
- new speciese5
- Comparator
- other known betasatellitese5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“During 2006-2007, ornamental rose plants (Rosa chinensis) were found with highly stunted growth and upward leaf curling in Faisalabad, Pakistan”
e5“One betasatellite isolated from these hosts showed less than 78% identity with other known betasatellites and can therefore be considered as a member of a new species”
Claim · model-written summary
The second betasatellite isolated with Eclipta yellow vein virus is an isolate of Kenaf leaf curl betasatellite.
- Taxon
- Eclipta prostrataEclipta prostratae1
- Interactor
- Kenaf leaf curl betasatelliteKenaf leaf curl betasatellitee6other
- Outcome
- isolate of the speciese6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Another commonly growing weed (Eclipta prostrata) showing vein yellowing was also analyzed for begomovirus infection”
e6“The second betasatellite isolated with EcYVV was found to be an isolate of the species Kenaf leaf curl betasatellite”
Claim · model-written summary
Agrobacterium inoculation of the infectious molecules induced severe vein yellowing and leaf curling in Nicotiana benthamiana.
- Taxon
- Nicotiana benthamiana
Nicotiana benthamianae7 - Treatment
- inoculated through Agrobacteriume7
- Interactor
- virus/satellitee7pathogen
- Outcome
- vein yellowing and leaf curlinge7
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e7“The infectious molecules for each of the virus/satellite were prepared and inoculated through Agrobacterium to model host Nicotiana benthamiana plants, which induced severe vein yellowing and leaf curling”
Claim · model-written summary
Betasatellites from this study are trans-replicated by a non-cognate, already known bipartite virus, Tomato leaf curl New Delhi virus.
- Taxon
- Nicotiana benthamianaNicotiana benthamianae7
- Interactor
- betasatellitesBetasatellitee0other
- Second interactor
- Tomato leaf curl New Delhi virusTomato leaf curl New Delhi viruse0pathogen
- Outcome
- trans-replicatede0
- Comparator
- non-cognate, already known bipartite viruse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The betasatellites identified in this study are promiscuous in their ability to be trans-replicated by a non-cognate, already known bipartite virus; Tomato leaf curl New Delhi virus”
e7“The infectious molecules for each of the virus/satellite were prepared and inoculated through Agrobacterium to model host Nicotiana benthamiana plants, which induced severe vein yellowing and leaf curling”
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See all 40 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.