Abstract
The present research investigates the interactive effects of 24-epibrassinolide (0, 0.02 and 0.5 µM) plus thiamine (0 and 200 µM) treatments on some of physio-biochemical parameters in Brassica napus plants under Cadmium Chloride toxic levels; 250 and 500 µM. Application of 24-epibrassinolide 0.02 µM plus thiamine 200 µM significantly increased growth and biochemical parameters such as shoot length, shoot dry weight and total chlorophyll content compared to the Cd stress conditions. While, reduced oxidative markers such as malondialdehyde, hydrogen peroxide contents and leaves Cd accumulation. Apply of 24-epibrassinolide plus thiamine increased non-enzymatic antioxidant contents and activity of antioxidant enzymes such as catalase, ascorbate peroxidase and guaiacol peroxidase. Also, enhancing the DPPH radical scavenging potential along with increasing PAL activity indicates the major influence of 24-epibrassinolide 0.02 µM and thiamine 200 µM in the reduction of Cd-induced oxidative stress in canola plants. Also In the present of 250 and 500 µM Cd, EBL 0.02 μM plus thiamine 200 μM significantly decreased H2O2 accumulation by 49% and 38% compared to 250 and 500 µM Cd treatment without applying the plant growth regulators respectively. Generally, Application of EBL 0.02 μM plus thiamine 200 μM improved the oxidative resistance of Canola plant under cadmium stress 250 µM.
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Claims 8
Claim · model-written summary
The combined treatment increased shoot length, shoot dry weight and chlorophyll.
- Taxon
- Brassica napus
_Brassica_napus_-_Habit.jpg?width=60)
Brassica napust0 - Treatment
- 24-epibrassinolide 0.02 µM plus thiamine 200 µMe0
- Outcome
- shoot length, shoot dry weight and total chlorophyll contente0
- Direction
- increasede0
- Comparator
- Cd stress conditionse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_Brassica_napus_-_Habit.jpg?width=500)
Verbatim
e0“Application of 24-epibrassinolide 0.02 µM plus thiamine 200 µM significantly increased growth and biochemical parameters such as shoot length, shoot dry weight and total chlorophyll content compared to the Cd stress conditions”
t0“Brassica napus L.”
Claim · model-written summary
The treatment reduced malondialdehyde, hydrogen peroxide and leaf cadmium.
- Taxon
- Brassica napusBrassica napust0
- Treatment
- 24-epibrassinolide 0.02 µM plus thiamine 200 µMe0
- Outcome
- malondialdehyde, hydrogen peroxide contents and leaves Cd accumulatione0
- Direction
- reducede0
- Comparator
- Cd stress conditionse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Application of 24-epibrassinolide 0.02 µM plus thiamine 200 µM significantly increased growth and biochemical parameters such as shoot length, shoot dry weight and total chlorophyll content compared to the Cd stress conditions. While, reduced oxidative markers such as malondialdehyde, hydrogen peroxide contents and leaves Cd accumulation”
t0“Brassica napus L.”
Claim · model-written summary
The treatment increased non-enzymatic antioxidants.
- Taxon
- Brassica napusBrassica napust0
- Treatment
- 24-epibrassinolide plus thiaminee0
- Outcome
- non-enzymatic antioxidant contentse0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Apply of 24-epibrassinolide plus thiamine increased non-enzymatic antioxidant contents and activity of antioxidant enzymes such as catalase, ascorbate peroxidase and guaiacol peroxidase”
t0“Brassica napus L.”
Claim · model-written summary
The treatment increased catalase, ascorbate peroxidase and guaiacol peroxidase activity.
- Taxon
- Brassica napusBrassica napust0
- Treatment
- 24-epibrassinolide plus thiaminee0
- Outcome
- activity of antioxidant enzymes such as catalase, ascorbate peroxidase and guaiacol peroxidasee0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Apply of 24-epibrassinolide plus thiamine increased non-enzymatic antioxidant contents and activity of antioxidant enzymes such as catalase, ascorbate peroxidase and guaiacol peroxidase”
t0“Brassica napus L.”
Claim · model-written summary
The treatment increased DPPH scavenging and PAL activity.
- Taxon
- canola
.jpg?width=60)
Brassica napus var. napuse0 - Treatment
- 24-epibrassinolide 0.02 µM and thiamine 200 µMe0
- Outcome
- DPPH radical scavenging potential along with increasing PAL activitye0
- Direction
- enhancinge0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“enhancing the DPPH radical scavenging potential along with increasing PAL activity indicates the major influence of 24-epibrassinolide 0.02 µM and thiamine 200 µM in the reduction of Cd-induced oxidative stress in canola plants”
Claim · model-written summary
The treatment decreased H2O2 by 49% at 250 µM cadmium.
- Taxon
- Brassica napusBrassica napust0
- Treatment
- EBL 0.02 μM plus thiamine 200 μMe0
- Outcome
- H2O2 accumulatione0
- Direction
- decreasede0
- Value
- 49%e0
- Comparator
- Cd treatment without applying the plant growth regulatorse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In the present of 250 and 500 µM Cd, EBL 0.02 μM plus thiamine 200 μM significantly decreased H2O2 accumulation by 49% and 38% compared to 250 and 500 µM Cd treatment without applying the plant growth regulators respectively”
t0“Brassica napus L.”
Claim · model-written summary
The treatment decreased H2O2 by 38% at 500 µM cadmium.
- Taxon
- Brassica napusBrassica napust0
- Treatment
- EBL 0.02 μM plus thiamine 200 μMe0
- Outcome
- H2O2 accumulatione0
- Direction
- decreasede0
- Value
- 38%e0
- Comparator
- 500 µM Cd treatment without applying the plant growth regulatorse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In the present of 250 and 500 µM Cd, EBL 0.02 μM plus thiamine 200 μM significantly decreased H2O2 accumulation by 49% and 38% compared to 250 and 500 µM Cd treatment without applying the plant growth regulators respectively”
t0“Brassica napus L.”
Claim · model-written summary
The treatment improved oxidative resistance at 250 µM cadmium.
- Taxon
- CanolaBrassica napus var. napuse0
- Treatment
- EBL 0.02 μM plus thiamine 200 μMe0
- Outcome
- oxidative resistancee0
- Direction
- improvede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Application of EBL 0.02 μM plus thiamine 200 μM improved the oxidative resistance of Canola plant under cadmium stress 250 µM”
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