Abstract
Sugar transporters of the SWEET family mediate cross membrane movement of mono- and disaccharides and play vital roles in diverse physiological and pathophysiological processes, including sink–source relationship, pathogen responses, reproductive growth, and development. However, it remains to be determined how these transporters function in non-module plants of agricultural significance, given the evolutionarily diverse traits. In this study, we combined transcriptome analysis, rapid amplification of cDNA ends-cloning (RACE-cloning), expression profiling, and heterologous functional assay to identify SWEET genes that may have potential roles during flower opening and sexual reproduction in Jasminum sambac . During the anthesis, the floral organs of J. sambac express seven SWEET homologous genes from all four clades of the family. JsSWEET9 and 2 are significantly upregulated when flowers are fully opened, up to 6- and 3-fold compared to unopened buds, respectively. The other transporters, JsSWEET1, 5, 10, and 17 are also accumulated slightly at stage associated with fragrance release, whereas only the vacuole transporter JsSWEET16 showed small decrease in transcript level after anthesis. The JsSWEET5, a clade II member, is capable to complement yeast cell uptake on most tested sugar substrates with a preference for hexoses, while the clade I transporter JsSWEET1 mediates merely galactose import when expressed in yeast. Our results provide first evidence for further investigation on sugar transport and allocation during flowering and reproductive processes in J. sambac.
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Claims 7
Claim · model-written summary
During anthesis the floral organs of J. sambac express seven SWEET homologous genes from all four clades of the family.
- Taxon
- J. sambac
Jasminum sambace0 - Organ / stage
- floral organse0
- Gene
- SWEET homologous genese0
- Outcome
- express seven SWEET homologous genese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“During the anthesis, the floral organs of J. sambac express seven SWEET homologous genes from all four clades of the family”
Claim · model-written summary
JsSWEET9 was significantly upregulated, up to 6-fold, in fully opened flowers compared with unopened buds.
- Taxon
- Jasminum sambacJasminum sambact0
- Organ / stage
- flowers are fully openede0
- Gene
- JsSWEET9e0
- Outcome
- upregulatede0
- Direction
- upregulatede0
- Value
- 6-e0
- Comparator
- unopened budse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“JsSWEET9 and 2 are significantly upregulated when flowers are fully opened, up to 6- and 3-fold compared to unopened buds, respectively”
t0“Jasminum sambac”
Claim · model-written summary
JsSWEET2 was significantly upregulated, up to 3-fold, in fully opened flowers compared with unopened buds.
- Taxon
- Jasminum sambacJasminum sambact0
- Organ / stage
- flowers are fully openede0
- Gene
- JsSWEET9 and 2e0
- Outcome
- upregulatede0
- Direction
- upregulatede0
- Value
- 3-folde0
- Comparator
- unopened budse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“JsSWEET9 and 2 are significantly upregulated when flowers are fully opened, up to 6- and 3-fold compared to unopened buds, respectively”
t0“Jasminum sambac”
Claim · model-written summary
JsSWEET1, 5, 10 and 17 accumulated slightly at the stage associated with fragrance release.
- Taxon
- Jasminum sambacJasminum sambact0
- Gene
- JsSWEET1, 5, 10, and 17e0
- Outcome
- accumulated slightly at stage associated with fragrance releasee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The other transporters, JsSWEET1, 5, 10, and 17 are also accumulated slightly at stage associated with fragrance release”
t0“Jasminum sambac”
Claim · model-written summary
Transcript level of the vacuole transporter JsSWEET16 showed a small decrease after anthesis.
- Taxon
- Jasminum sambacJasminum sambact0
- Gene
- JsSWEET16e0
- Outcome
- transcript levele0
- Direction
- small decreasee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“only the vacuole transporter JsSWEET16 showed small decrease in transcript level after anthesis”
t0“Jasminum sambac”
Claim · model-written summary
When expressed in yeast, JsSWEET5 complemented cell uptake of most tested sugar substrates, with a preference for hexoses.
- Taxon
- Jasminum sambacJasminum sambact0
- Gene
- JsSWEET5e0
- Outcome
- complement yeast cell uptake on most tested sugar substratese0
- Qualifier
- preference for hexosese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The JsSWEET5, a clade II member, is capable to complement yeast cell uptake on most tested sugar substrates with a preference for hexoses”
t0“Jasminum sambac”
Claim · model-written summary
The clade I transporter JsSWEET1 mediated only galactose import when expressed in yeast.
- Taxon
- Jasminum sambacJasminum sambact0
- Gene
- JsSWEET1e0
- Outcome
- mediates merely galactose importe0
- Qualifier
- expressed in yeaste0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the clade I transporter JsSWEET1 mediates merely galactose import when expressed in yeast”
t0“Jasminum sambac”