Abstract
[Truncated] Plant mitochondria contain a branched respiratory electron transport chain that terminates in two terminal oxidases, the cytochrome oxidase and the alternative oxidase (AOX). Electron transport through the cytochrome oxidase pathway results in the formation of a proton motive force that can be used to drive ATP synthesis. AOX, however, directly oxidises ubiquinol and thus bypasses two proton translocation sites. This pathway mediates non-coupled respiratory electron transport. Plant mitochondria also contain an uncoupling protein (UCP), which dissipates the proton motive force as it is generated, resulting in uncoupled respiratory electron transport. This project aimed to investigate the coordination of expression of the UCP and AOX proteins as well as the role of a highly conserved cysteine residue in the regulation of AOX activity. The coordination of UCP and AOX protein expression was investigated in ripening and chilled tomato (Lycopersicon esculentum L.) fruit, two situations that have previously been shown to induce AOX expression in other species. The peak in expression of both proteins was found to occur after the respiratory climacteric in tomato. The abundance of bothproteins was also found to increase in response to chilling of mature green tomatoes. A multigene family for AOX was cloned from a tomato fruit cDNA library.
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Claims 3
Claim · model-written summary
UCP and AOX protein expression peaked after the respiratory climacteric in tomato.
- Taxon
- tomato
Solanum lycopersicume0 - Organ / stage
- after the respiratory climacterice0
- Gene
- both proteinse0
- Outcome
- peak in expressione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The peak in expression of both proteins was found to occur after the respiratory climacteric in tomato.”
Claim · model-written summary
Chilling increased the abundance of both respiratory proteins in mature green tomatoes.
- Taxon
- tomatoesSolanum lycopersicume0
- Organ / stage
- mature greene0
- Treatment
- chillinge0
- Gene
- bothproteinse0
- Outcome
- abundance of bothproteinse0
- Direction
- increasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The abundance of bothproteins was also found to increase in response to chilling of mature green tomatoes.”
Claim · model-written summary
A multigene AOX family was cloned from a tomato fruit cDNA library.
- Taxon
- tomatoSolanum lycopersicume0
- Organ / stage
- fruite0
- Gene
- AOXe0
- Outcome
- multigene family for AOXe0
Not reportedComparatorValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A multigene family for AOX was cloned from a tomato fruit cDNA library.”
The same questions in other papers
cold · pest · Solanum lycopersicum (tomato)Positive and negative results
3 papers in the same direction as this paper · 2 in the opposite direction
- negative
- negative
- positive
2 papers more in this group
See all 6 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.