Abstract
Activation and enhancement of heat shock factor (HSF) pathways are important adaptive responses to heat stress in plants. The γ-aminobutyric acid (GABA) plays an important role in regulating heat tolerance, but it is unclear whether GABA-induced thermotolerance is associated with activation of HSF pathways in plants. In this study, the changes of endogenous GABA level affecting physiological responses and genes involved in HSF pathways were investigated in creeping bentgrass during heat stress. The increase in endogenous GABA content induced by exogenous application of GABA effectively alleviated heat damage, as reflected by higher leaf relative water content, cell membrane stability, photosynthesis, and lower oxidative damage. Contrarily, the inhibition of GABA accumulation by the application of GABA biosynthesis inhibitor further aggravated heat damage. Transcriptional analyses showed that exogenous GABA could significantly upregulate transcript levels of genes encoding heat shock factor HSFs (HSFA-6a, HSFA-2c, and HSFB-2b), heat shock proteins (HSP17.8, HSP26.7, HSP70, and HSP90.1-b1), and ascorbate peroxidase 3 (APX3), whereas the inhibition of GABA biosynthesis depressed these genes expression under heat stress. Our results indicate GABA regulates thermotolerance associated with activation and enhancement of HSF pathways in creeping bentgrass.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 9
Claim · model-written summary
Exogenous GABA raised endogenous GABA and alleviated heat damage.
- Taxon
- Creeping Bentgrass
Agrostis stoloniferat0 - Treatment
- exogenous application of GABAe0
- Outcome
- heat damagee0
- Direction
- alleviatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The increase in endogenous GABA content induced by exogenous application of GABA effectively alleviated heat damage”
t0“Creeping Bentgrass”
Claim · model-written summary
GABA treatment increased leaf relative water content, membrane stability and photosynthesis during heat stress.
- Taxon
- Creeping BentgrassAgrostis stoloniferat0
- Outcome
- leaf relative water content, cell membrane stability, photosynthesise0
- Direction
- highere0
Not reportedTreatmentComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“as reflected by higher leaf relative water content, cell membrane stability, photosynthesis, and lower oxidative damage”
t0“Creeping Bentgrass”
Claim · model-written summary
GABA treatment reduced oxidative damage during heat stress.
- Taxon
- Creeping BentgrassAgrostis stoloniferat0
- Outcome
- oxidative damagee0
- Direction
- lowere0
Not reportedTreatmentComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“as reflected by higher leaf relative water content, cell membrane stability, photosynthesis, and lower oxidative damage”
t0“Creeping Bentgrass”
Claim · model-written summary
Inhibiting GABA accumulation aggravated heat damage.
- Taxon
- Creeping BentgrassAgrostis stoloniferat0
- Treatment
- GABA biosynthesis inhibitore0
- Outcome
- heat damagee0
- Direction
- aggravatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the inhibition of GABA accumulation by the application of GABA biosynthesis inhibitor further aggravated heat damage”
t0“Creeping Bentgrass”
Claim · model-written summary
Exogenous GABA significantly upregulated HSFA-6a, HSFA-2c and HSFB-2b transcripts during heat stress.
- Taxon
- Creeping BentgrassAgrostis stoloniferat0
- Treatment
- exogenous GABAe0
- Gene
- HSFA-6a, HSFA-2c, and HSFB-2be0
- Outcome
- transcript levelse0
- Direction
- upregulatee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“exogenous GABA could significantly upregulate transcript levels of genes encoding heat shock factor HSFs (HSFA-6a, HSFA-2c, and HSFB-2b)”
t0“Creeping Bentgrass”
Claim · model-written summary
Exogenous GABA significantly upregulated HSP17.8, HSP26.7 and HSP70 transcripts during heat stress.
- Taxon
- Creeping BentgrassAgrostis stoloniferat0
- Treatment
- exogenous GABAe0
- Gene
- HSP17.8, HSP26.7, HSP70e0
- Outcome
- transcript levelse0
- Direction
- upregulatee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“exogenous GABA could significantly upregulate transcript levels of genes encoding heat shock factor HSFs (HSFA-6a, HSFA-2c, and HSFB-2b), heat shock proteins (HSP17.8, HSP26.7, HSP70, and HSP90.1-b1)”
t0“Creeping Bentgrass”
Claim · model-written summary
Exogenous GABA significantly upregulated HSP90.1-b1 and APX3 transcripts during heat stress.
- Taxon
- Creeping BentgrassAgrostis stoloniferat0
- Treatment
- exogenous GABAe0
- Gene
- APX3e0
- Outcome
- transcript levelse0
- Direction
- upregulatee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“exogenous GABA could significantly upregulate transcript levels of genes encoding heat shock factor HSFs (HSFA-6a, HSFA-2c, and HSFB-2b), heat shock proteins (HSP17.8, HSP26.7, HSP70, and HSP90.1-b1), and ascorbate peroxidase 3 (APX3)”
t0“Creeping Bentgrass”
Claim · model-written summary
GABA biosynthesis inhibition depressed expression of HSF, HSP and APX3 genes under heat stress.
- Taxon
- Creeping BentgrassAgrostis stoloniferat0
- Treatment
- inhibition of GABA biosynthesise0
- Outcome
- these genes expressione0
- Direction
- depressede0
- Qualifier
- under heat stresse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the inhibition of GABA biosynthesis depressed these genes expression under heat stress”
t0“Creeping Bentgrass”
Claim · model-written summary
The authors infer GABA mediated thermotolerance is associated with HSF pathway activation.
- Taxon
- creeping bentgrassAgrostis stoloniferae0
- Compound
- GABAe0
- Outcome
- thermotolerancee0
- Qualifier
- activation and enhancement of HSF pathwayse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GABA regulates thermotolerance associated with activation and enhancement of HSF pathways in creeping bentgrass”
The same questions in other papers
gamma-aminobutyric acid · pest · Agrostis stolonifera (creeping bent grass)Results agree
1 paper in the same direction as this paper
- positive
gamma-aminobutyric acid · molecular: expression · Agrostis stolonifera (creeping bent grass)Results agree
5 papers in the same direction as this paper
- positive
- positive
- positive
2 papers more in this group
See all 6 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.