Abstract
Alfalfa (Medicago sativa L.) is a valuable forage legume, but its production is largely affected by high temperature. In this study, we investigated the effect of heat stress on 15 alfalfa cultivars to identify heat-tolerant and -sensitive cultivars. Seedlings were exposed to 38/35 °C day/night temperature for 7 days and various parameters were measured. Heat stress significantly reduced the biomass, relative water content (RWC), chlorophyll content, and increased the electrolyte leakage (EL) and malondialdehyde (MDA) content of heat-sensitive alfalfa cultivars. However, heat-tolerant cultivars showed higher soluble sugar (SS) and soluble protein (SP) content. The heat tolerance of each cultivar was comprehensively evaluated based on membership function value. Cultivars with higher mean membership function value of 0.86 (Bara310SC) and 0.80 (Magna995) were heat tolerant, and Gibraltar and WL712 with lower membership function value (0.24) were heat sensitive. The heat tolerance of the above four cultivars were further evaluated by chlorophyll a fluorescence analysis. Heat stress significantly affected the photosynthetic activity of heat-sensitive cultivars. The overall results indicate that Bara310SC and WL712 are heat-tolerant and heat-sensitive cultivars, respectively. This study provides basic information for understanding the effect of heat stress on growth and productivity of alfalfa.
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Claims 7
Claim · model-written summary
Heat significantly reduced biomass, relative water content and chlorophyll in sensitive alfalfa cultivars.
- Taxon
- alfalfa
Medicago sativae0 - Treatment
- Heat stresse0
- Outcome
- biomass, relative water content (RWC), chlorophyll contente0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“Heat stress significantly reduced the biomass, relative water content (RWC), chlorophyll content, and increased the electrolyte leakage (EL) and malondialdehyde (MDA) content of heat-sensitive alfalfa cultivars.”
Claim · model-written summary
Heat significantly increased electrolyte leakage and malondialdehyde in sensitive cultivars.
- Taxon
- alfalfa
Medicago sativae0 - Treatment
- Heat stresse0
- Outcome
- electrolyte leakage (EL) and malondialdehyde (MDA) contente0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“Heat stress significantly reduced the biomass, relative water content (RWC), chlorophyll content, and increased the electrolyte leakage (EL) and malondialdehyde (MDA) content of heat-sensitive alfalfa cultivars.”
Claim · model-written summary
Heat-tolerant cultivars had higher soluble sugar and protein content.
- Taxon
- alfalfa
Medicago sativat0 - Material
- heat-tolerant cultivarse0
- Outcome
- soluble sugar (SS) and soluble protein (SP) contente0
- Direction
- highere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“heat-tolerant cultivars showed higher soluble sugar (SS) and soluble protein (SP) content.”
t0“Alfalfa”
Claim · model-written summary
Bara310SC was heat tolerant with mean membership value 0.86.
- Taxon
- Alfalfa
Medicago sativat0 - Material
- Bara310SCe0
- Outcome
- heat tolerante0
- Value
- 0.86e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“higher mean membership function value of 0.86 (Bara310SC) and 0.80 (Magna995) were heat tolerant”
t0“Alfalfa”
Claim · model-written summary
Magna995 was heat tolerant with mean membership value 0.80.
- Taxon
- Alfalfa
Medicago sativat0 - Material
- Magna995e0
- Outcome
- heat tolerante0
- Value
- 0.80e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“higher mean membership function value of 0.86 (Bara310SC) and 0.80 (Magna995) were heat tolerant”
t0“Alfalfa”
Claim · model-written summary
Gibraltar and WL712 were heat sensitive with lower membership value 0.24.
- Taxon
- Alfalfa
Medicago sativat0 - Material
- Gibraltar and WL712e0
- Outcome
- heat sensitivee0
- Value
- 0.24e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“Gibraltar and WL712 with lower membership function value (0.24) were heat sensitive.”
t0“Alfalfa”
Claim · model-written summary
Heat significantly affected photosynthetic activity in sensitive cultivars.
- Taxon
- Alfalfa
Medicago sativat0 - Treatment
- Heat stresse0
- Outcome
- photosynthetic activitye0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“Heat stress significantly affected the photosynthetic activity of heat-sensitive cultivars.”
t0“Alfalfa”
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- negative
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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.