Paper · 2019 · IOP Conference Series Earth and Environmental Science

Ion-exchange substrates as the basis for growing seedlings of potato in tube culture

S V Filippova; L V Eliseeva; G A Mefodev; et al.

6
claims
12
verbatim quotes
1
citations
2
research areas
✓
Open access · cc-by

Abstract

Abstract Studies had been carried out to study the possibility of using ionite substrates for growing potato seedlings in tube culture, to analyze the variability of hybrid potato populations by morphobiological and economically valuable characteristics depending on the substrate, to analyze the effectiveness of the selection of high-yielding forms at the early stage of the breeding process. The experiment included 7 different modifications of the nutrient medium composition. Seed germination, seedling survival rate, tuber formation rate, percentage of tuber-forming seedlings, number of tubers per seedling, size, and shape of tubers were taken into account. Quantitative assessment of factors in phenotypic manifestation of signs was carried out by the method of dispersion analysis. Sterile microtubers were stored at 2-4°C in the dark before planting. As a result of the research, a different tuber-forming ability was found in seedlings of populations of different variants. The tube culture of seedlings ensures full survival of seedlings. For the growth and development of seedlings in a tube culture, the best of those studied was the nutrient medium based on the ion-exchange substrate IS-2V, containing 1% sucrose and 500 mg / 1 CCC. Two-stage cultivation of seedlings (the initial increase in the above-ground mass, then the induction of tuberization) had a positive effect on the pace and quality of tuberization. 30–40 days after induction, tuberization was observed in 90–100% of seedlings, regardless of their origin, and the size of microtubers was on average 6.7–8.7 mm.

Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗

The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.

Claims 6

Observationobservedoriginal resultmeasured

Claim · model-written summary

Tuber formation ability differed among potato seedling populations.

Material
populations of different variantse0
Outcome
tuber-forming abilitye0
Direction
differente0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum tuberosum
Solanum tuberosum · TaxonScott Bauer, USDA ARS · Public domain · Wikimedia Commons ↗

Verbatim

e0

“a different tuber-forming ability was found in seedlings of populations of different variants.”

✓ located verbatim in the source · abstract, characters 891–985 · show in text ↑
t0

“potato”

✓ located verbatim in the source · title, characters 62–68 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Potato seedlings had full survival in tube culture.

Outcome
full survival of seedlingse0
Setting
tube culturee0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum tuberosum
Solanum tuberosum · TaxonScott Bauer, USDA ARS · Public domain · Wikimedia Commons ↗

Verbatim

e0

“The tube culture of seedlings ensures full survival of seedlings.”

✓ located verbatim in the source · abstract, characters 986–1051 · show in text ↑
t0

“potato”

✓ located verbatim in the source · title, characters 62–68 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

IS-2V medium containing sucrose and CCC was best for potato seedling growth and development among media studied.

Treatment
nutrient medium based on the ion-exchange substrate IS-2V, containing 1% sucrose and 500 mg / 1 CCCe0
Outcome
growth and development of seedlingse0
Direction
beste0
Comparator
those studiede0
Setting
tube culturee0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum tuberosum
Solanum tuberosum · TaxonScott Bauer, USDA ARS · Public domain · Wikimedia Commons ↗

Verbatim

e0

“For the growth and development of seedlings in a tube culture, the best of those studied was the nutrient medium based on the ion-exchange substrate IS-2V, containing 1% sucrose and 500 mg / 1 CCC.”

✓ located verbatim in the source · abstract, characters 1052–1249 · show in text ↑
t0

“potato”

✓ located verbatim in the source · title, characters 62–68 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Two-stage cultivation improved the pace and quality of potato tuberization.

Treatment
Two-stage cultivation of seedlingse0
Outcome
pace and quality of tuberizatione0
Direction
positive effecte0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum tuberosum
Solanum tuberosum · TaxonScott Bauer, USDA ARS · Public domain · Wikimedia Commons ↗

Verbatim

e0

“Two-stage cultivation of seedlings (the initial increase in the above-ground mass, then the induction of tuberization) had a positive effect on the pace and quality of tuberization.”

✓ located verbatim in the source · abstract, characters 1250–1431 · show in text ↑
t0

“potato”

✓ located verbatim in the source · title, characters 62–68 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Tuberization occurred in 90–100% of potato seedlings 30–40 days after induction regardless of origin.

Outcome
tuberizatione0
Value
90–100%e0
Qualifier
30–40 days after inductione0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum tuberosum
Solanum tuberosum · TaxonScott Bauer, USDA ARS · Public domain · Wikimedia Commons ↗

Verbatim

e0

“30–40 days after induction, tuberization was observed in 90–100% of seedlings, regardless of their origin”

✓ located verbatim in the source · abstract, characters 1432–1537 · show in text ↑
t0

“potato”

✓ located verbatim in the source · title, characters 62–68 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Potato microtubers averaged 6.7–8.7 mm.

Outcome
size of microtuberse0
Value
6.7–8.7 mme0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum tuberosum
Solanum tuberosum · TaxonScott Bauer, USDA ARS · Public domain · Wikimedia Commons ↗

Verbatim

e0

“the size of microtubers was on average 6.7–8.7 mm.”

✓ located verbatim in the source · abstract, characters 1543–1593 · show in text ↑
t0

“potato”

✓ located verbatim in the source · title, characters 62–68 · show in text ↑

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