Abstract
As an obligate parasite, Puccinia striiformis f. sp. tritici (Pst) forms haustoria to obtain nutrients from plant cells for development, and these structures are essential for pathogen survival. To better understand the contribution of haustoria to the interactions with the host plants, we isolated haustoria from susceptible wheat leaves infected with Pst race CYR31 and sequenced their transcriptome as well as those of urediospores and germ tubes, and compared the three transcriptomes. A total of 3524 up-regulated genes were obtained from haustoria, of which 73 genes were related to thiamine biosynthesis, glycolysis and lipid metabolic processes. Silencing seven of the genes reduced the growth and development of Pst in wheat. More interestingly, 1197 haustorial secreted proteins (HASPs) were detected in haustoria, accounting for 34% of the total proteins, indicating that these HASPs play important roles in haustorium-mediated pathogenic progression. Furthermore, 69 HASPs were able to suppress Bax-triggered programmed cell death in tobacco. Additionally, 46 HASPs significantly reduced callose deposition in wheat using the type III secretion system. This study identified a large number of effectors through transcriptome sequencing, and the results revealed components of metabolic pathways that impact the growth and colonization of the pathogen and indicate essential functions of haustoria in the growth and pathogenicity of Pst.
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Claims 8
Claim · model-written summary
Haustoria of Pst on wheat showed 3524 up-regulated genes compared with urediospores and germ tubes.
- Taxon
- wheat
Triticum aestivume0 - Organ / stage
- haustoriae1
- Interactor
- Pste0pathogen
- Outcome
- up-regulated genese1
- Value
- 3524e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we isolated haustoria from susceptible wheat leaves infected with Pst race CYR31”
e1“A total of 3524 up-regulated genes were obtained from haustoria, of which 73 genes were related to thiamine biosynthesis, glycolysis and lipid metabolic processes”
Claim · model-written summary
Among the haustorial up-regulated genes, 73 were related to thiamine biosynthesis, glycolysis and lipid metabolic processes.
- Taxon
- wheatTriticum aestivume0
- Organ / stage
- haustoriae1
- Interactor
- Pste0pathogen
- Outcome
- thiamine biosynthesis, glycolysis and lipid metabolic processese1
- Value
- 73 genese1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we isolated haustoria from susceptible wheat leaves infected with Pst race CYR31”
e1“A total of 3524 up-regulated genes were obtained from haustoria, of which 73 genes were related to thiamine biosynthesis, glycolysis and lipid metabolic processes”
Claim · model-written summary
Silencing seven of the genes reduced growth and development of Pst in wheat.
- Taxon
- wheatTriticum aestivume2
- Treatment
- Silencing seven of the genese2
- Interactor
- Pste2pathogen
- Outcome
- growth and development of Pste2
- Direction
- reducede2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“Silencing seven of the genes reduced the growth and development of Pst in wheat”
Claim · model-written summary
1197 haustorial secreted proteins (HASPs) were detected in haustoria of Pst on wheat.
- Taxon
- wheatTriticum aestivume0
- Organ / stage
- haustoriae3
- Interactor
- Pste0pathogen
- Gene
- HASPse3
- Outcome
- haustorial secreted proteinse3
- Value
- 1197e3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we isolated haustoria from susceptible wheat leaves infected with Pst race CYR31”
e3“1197 haustorial secreted proteins (HASPs) were detected in haustoria, accounting for 34% of the total proteins”
Claim · model-written summary
HASPs make up 34% of the total haustorial proteins.
- Taxon
- wheatTriticum aestivume0
- Interactor
- Pste0pathogen
- Gene
- HASPse3
- Outcome
- accounting for 34% of the total proteinse3
- Value
- 34%e3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we isolated haustoria from susceptible wheat leaves infected with Pst race CYR31”
e3“1197 haustorial secreted proteins (HASPs) were detected in haustoria, accounting for 34% of the total proteins”
Claim · model-written summary
69 HASPs suppressed Bax-triggered programmed cell death in tobacco.
- Taxon
- wheatTriticum aestivume0
- Treatment
- 69 HASPse4
- Interactor
- Pste0pathogen
- Gene
- HASPse4
- Outcome
- Bax-triggered programmed cell deathe4
- Direction
- suppresse4
- Qualifier
- in tobaccoe4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we isolated haustoria from susceptible wheat leaves infected with Pst race CYR31”
e4“69 HASPs were able to suppress Bax-triggered programmed cell death in tobacco”
Claim · model-written summary
46 HASPs significantly reduced callose deposition in wheat using the type III secretion system.
- Taxon
- wheatTriticum aestivume5
- Treatment
- type III secretion systeme5
- Interactor
- Pste0pathogen
- Gene
- HASPse5
- Outcome
- callose depositione5
- Direction
- reducede5
- Value
- 46 HASPse5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we isolated haustoria from susceptible wheat leaves infected with Pst race CYR31”
e5“46 HASPs significantly reduced callose deposition in wheat using the type III secretion system”
Claim · model-written summary
Metabolic pathway components of Pst are indicated to affect its growth and colonization of wheat.
- Taxon
- wheatTriticum aestivume0
- Outcome
- growth and colonization of the pathogene6
- Qualifier
- components of metabolic pathwayse6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we isolated haustoria from susceptible wheat leaves infected with Pst race CYR31”
e6“the results revealed components of metabolic pathways that impact the growth and colonization of the pathogen”
The same questions in other papers
loss of function · growth · Triticum aestivum (wheat)Positive and negative results
17 papers in the same direction as this paper · 21 in the opposite direction
- positive
- negative
- negative
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See all 39 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.