Abstract
Puccinia striiformis f. sp. tritici (Pst) secretes effector proteins that enter plant cells to manipulate host immune processes. In this report, we present an important Pst effector, Pst03724, whose mRNA expression level increases during Pst infection of wheat (Triticum aestivum). Silencing of Pst03724 reduced the growth and development of Pst. Pst03724 targeted the wheat calmodulin TaCaM3-2B, a positive regulator of wheat immunity. Subsequent investigations revealed that Pst03724 interferes with the TaCaM3-2B-NAD kinase (NADK) TaNADK2 association and thus inhibits the enzyme activity of TaNADK2 activated by TaCaM3-2B. Knocking down TaNADK2 expression by virus-mediated gene silencing significantly increased fungal growth and development, suggesting a decrease in resistance against Pst infection. In conclusion, our findings indicate that Pst effector Pst03724 inhibits the activity of NADK by interfering with the TaCaM3-2B-TaNADK2 association, thereby facilitating Pst infection.
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Claims 8
Claim · model-written summary
The mRNA level of the effector Pst03724 increases during Pst infection of wheat.
- Taxon
- wheat
Triticum aestivume2 - Treatment
- Pst infectione2
- Interactor
- Puccinia striiformis f. sp. tritici
Puccinia striiformis f. sp. triticie1pathogen - Gene
- Pst03724e2
- Outcome
- mRNA expression levele2
- Direction
- increasese2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Puccinia striiformis f. sp. tritici (Pst)”
e2“an important Pst effector, Pst03724, whose mRNA expression level increases during Pst infection of wheat”
Claim · model-written summary
Silencing of Pst03724 reduced the growth and development of the pathogen Pst.
- Taxon
- wheatTriticum aestivume2
- Treatment
- Silencing of Pst03724e3
- Interactor
- Pste3pathogen
- Gene
- Pst03724e3
- Outcome
- growth and development of Pste3
- Direction
- reducede3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“an important Pst effector, Pst03724, whose mRNA expression level increases during Pst infection of wheat”
e3“Silencing of Pst03724 reduced the growth and development of Pst”
Claim · model-written summary
The effector Pst03724 targets the wheat calmodulin TaCaM3-2B.
- Taxon
- wheatTriticum aestivume4
- Interactor
- Puccinia striiformis f. sp. triticiPuccinia striiformis f. sp. triticie1pathogen
- Gene
- TaCaM3-2Be4
- Outcome
- targeted the wheat calmodulin TaCaM3-2Be4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Puccinia striiformis f. sp. tritici (Pst)”
e4“Pst03724 targeted the wheat calmodulin TaCaM3-2B”
Claim · model-written summary
Pst03724 interferes with the association of TaCaM3-2B and TaNADK2 in wheat.
- Taxon
- wheatTriticum aestivume4
- Interactor
- Puccinia striiformis f. sp. triticiPuccinia striiformis f. sp. triticie1pathogen
- Gene
- TaNADK2e5
- Outcome
- TaCaM3-2B-NAD kinase (NADK) TaNADK2 associatione5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Puccinia striiformis f. sp. tritici (Pst)”
e4“Pst03724 targeted the wheat calmodulin TaCaM3-2B”
e5“interferes with the TaCaM3-2B-NAD kinase (NADK) TaNADK2 association”
Claim · model-written summary
Pst03724 inhibits the enzyme activity of TaNADK2 activated by TaCaM3-2B in wheat.
- Taxon
- wheatTriticum aestivume4
- Interactor
- Puccinia striiformis f. sp. triticiPuccinia striiformis f. sp. triticie1pathogen
- Gene
- TaNADK2e6
- Outcome
- enzyme activity of TaNADK2 activated by TaCaM3-2Be6
- Direction
- inhibitse6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Puccinia striiformis f. sp. tritici (Pst)”
e4“Pst03724 targeted the wheat calmodulin TaCaM3-2B”
e6“and thus inhibits the enzyme activity of TaNADK2 activated by TaCaM3-2B”
Claim · model-written summary
Knocking down TaNADK2 by virus-mediated silencing significantly increased fungal growth and development.
- Taxon
- wheatTriticum aestivume4
- Treatment
- Knocking down TaNADK2 expression by virus-mediated gene silencinge7
- Interactor
- Pste7pathogen
- Gene
- TaNADK2e7
- Outcome
- fungal growth and developmente7
- Direction
- increasede7
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e4“Pst03724 targeted the wheat calmodulin TaCaM3-2B”
e7“Knocking down TaNADK2 expression by virus-mediated gene silencing significantly increased fungal growth and development, suggesting a decrease in resistance against Pst infection”
Claim · model-written summary
Knocking down TaNADK2 is suggested to decrease resistance against Pst infection.
- Taxon
- wheatTriticum aestivume4
- Treatment
- Knocking down TaNADK2 expression by virus-mediated gene silencinge7
- Interactor
- Pste7pathogen
- Outcome
- resistance against Pst infectione7
- Direction
- decreasee7
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e4“Pst03724 targeted the wheat calmodulin TaCaM3-2B”
e7“Knocking down TaNADK2 expression by virus-mediated gene silencing significantly increased fungal growth and development, suggesting a decrease in resistance against Pst infection”
Claim · model-written summary
The authors conclude that Pst03724 facilitates Pst infection by interfering with the TaCaM3-2B-TaNADK2 association.
- Taxon
- wheatTriticum aestivume4
- Interactor
- Pste1pathogen
- Gene
- Pst03724e1
- Outcome
- facilitating Pst infectione1
- Qualifier
- by interfering with the TaCaM3-2B-TaNADK2 associatione1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e4“Pst03724 targeted the wheat calmodulin TaCaM3-2B”
e1“In conclusion, our findings indicate that Pst effector Pst03724 inhibits the activity of NADK by interfering with the TaCaM3-2B-TaNADK2 association, thereby facilitating Pst infection”
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