Abstract
Pathogens compromise host defense responses by strategically secreting effector proteins. However, the molecular mechanisms by which effectors manipulate disease-resistance factors to evade host surveillance remain poorly understood. In this study, we characterized a Puccinia striiformis f. sp. tritici (Pst) effector Pst21674 with a signal peptide. Pst21674 was significantly upregulated during Pst infections in wheat (Triticum aestivum L.) and knocking down Pst21674 by host-induced gene silencing led to reduced Pst pathogenicity and restricted hyphal spread in wheat. Pst21674 interaction with the abscisic acid-, stress-, and ripening-induced protein TaASR3 was validated mainly in the nucleus. Size exclusion chromatography, bimolecular fluorescence complementation, and luciferase complementation imaging assays confirmed that TaASR3 could form a functional tetramer. Virus-induced gene silencing and overexpression demonstrated that TaASR3 contributes to wheat resistance to stripe rust by promoting accumulation of reactive oxygen species and cell death. Additionally, transcriptome analysis revealed that the expression of defense-related genes was regulated in transgenic wheat plants overexpressing TaASR3. Interaction between Pst21674 and TaASR3 interfered with the polymerization of TaASR3 and suppressed TaASR3-mediated transcriptional activation of defense-related genes. These results indicate that Pst21674 serves as an important virulence factor secreted into the host nucleus to impede wheat resistance to Pst, possibly by targeting and preventing polymerization of TaASR3.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 10
Claim · model-written summary
Pst21674 was upregulated during Pst infection in wheat.
- Taxon
- wheat
Triticum aestivume0 - Interactor
- Pste0pathogen
- Gene
- Pst21674e0
- Outcome
- Pst21674e0
- Direction
- upregulatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Pst21674 was significantly upregulated during Pst infections in wheat (Triticum aestivum L.)”
Claim · model-written summary
Silencing Pst21674 reduced Pst pathogenicity in wheat.
- Taxon
- wheatTriticum aestivume0
- Treatment
- knocking down Pst21674 by host-induced gene silencinge0
- Interactor
- Pste0pathogen
- Gene
- Pst21674e0
- Outcome
- Pst pathogenicitye0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“knocking down Pst21674 by host-induced gene silencing led to reduced Pst pathogenicity and restricted hyphal spread in wheat”
Claim · model-written summary
Silencing Pst21674 restricted Pst hyphal spread in wheat.
- Taxon
- wheatTriticum aestivume0
- Treatment
- knocking down Pst21674 by host-induced gene silencinge0
- Interactor
- Pste0pathogen
- Outcome
- hyphal spreade0
- Direction
- restrictede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“knocking down Pst21674 by host-induced gene silencing led to reduced Pst pathogenicity and restricted hyphal spread in wheat”
Claim · model-written summary
Pst21674 interacts with TaASR3 mainly in the nucleus.
- Taxon
- wheatTriticum aestivume1
- Interactor
- Pste1pathogen
- Gene
- TaASR3e0
- Outcome
- Pst21674 interactione0
- Qualifier
- mainly in the nucleuse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Pst21674 interaction with the abscisic acid-, stress-, and ripening-induced protein TaASR3 was validated mainly in the nucleus”
e1“Pst infections in wheat”
Claim · model-written summary
TaASR3 forms a functional tetramer.
- Taxon
- wheatTriticum aestivume1
- Gene
- TaASR3e0
- Outcome
- functional tetramere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“TaASR3 could form a functional tetramer”
e1“wheat plants overexpressing TaASR3”
Claim · model-written summary
TaASR3 promotes wheat resistance to stripe rust.
- Taxon
- wheatTriticum aestivume0
- Interactor
- stripe ruste0pathogen
- Gene
- TaASR3e0
- Outcome
- wheat resistance to stripe ruste0
Not reportedTreatmentComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“TaASR3 contributes to wheat resistance to stripe rust by promoting accumulation of reactive oxygen species and cell death”
Claim · model-written summary
TaASR3 promotes reactive oxygen species accumulation and cell death.
- Taxon
- wheatTriticum aestivume0
- Interactor
- stripe ruste0pathogen
- Gene
- TaASR3e0
- Outcome
- accumulation of reactive oxygen species and cell deathe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“TaASR3 contributes to wheat resistance to stripe rust by promoting accumulation of reactive oxygen species and cell death”
Claim · model-written summary
TaASR3 overexpression regulated defense-related gene expression in transgenic wheat.
- Taxon
- wheatTriticum aestivume0
- Treatment
- overexpressing TaASR3e0
- Gene
- TaASR3e0
- Outcome
- expression of defense-related genese0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the expression of defense-related genes was regulated in transgenic wheat plants overexpressing TaASR3”
Claim · model-written summary
Pst21674 interaction with TaASR3 interfered with TaASR3 polymerization.
- Taxon
- wheatTriticum aestivume1
- Interactor
- stripe ruste1pathogen
- Gene
- TaASR3e0
- Outcome
- polymerization of TaASR3e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Interaction between Pst21674 and TaASR3 interfered with the polymerization of TaASR3”
e1“wheat resistance to stripe rust”
Claim · model-written summary
Pst21674 interaction with TaASR3 suppressed TaASR3-mediated defense gene transcription.
- Taxon
- wheatTriticum aestivume1
- Interactor
- stripe ruste1pathogen
- Gene
- TaASR3e0
- Outcome
- TaASR3-mediated transcriptional activation of defense-related genese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Interaction between Pst21674 and TaASR3 interfered with the polymerization of TaASR3 and suppressed TaASR3-mediated transcriptional activation of defense-related genes”
e1“wheat resistance to stripe rust”
The same questions in other papers
loss of function · disease · Triticum aestivum (wheat)Positive and negative results
80 papers in the same direction as this paper · 96 in the opposite direction
- negative
- negative
- negative
173 papers more in this group
See all 177 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.