Abstract
Wheat stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is one of the most destructive wheat diseases. The plant hormone abscisic acid (ABA) plays a key regulatory role in plant response to stress. ABA-, stress-, ripening-induced proteins (ASR) have been shown to be abundantly induced in response to biotic and abiotic stresses to protect plants from damage. However, the function of wheat ASR2-like protein (TaASR2L) in plants under biotic stress remains unclear. In this study, transient silencing of TaASR2L using a virus-induced gene silencing system substantially reduced wheat resistance to Pst. TaASR2L interaction with serine/arginine-rich splicing factor SR30-like (TaSR30) was validated mainly in the nucleus. Knockdown of TaSR30 expression substantially reduced wheat resistance to Pst. Overexpression of TaASR2L and TaSR30 demonstrated that they can promote the expression of ABA- and resistance-related genes to enhance wheat resistance to Pst. In addition, the expression levels of TaSR30 and TaASR2L were substantially increased by exogenous ABA, and the resistance of wheat to Pst was increased, and the expression of PR genes was induced. Therefore, these results suggest that TaASR2L interacts with TaSR30 by promoting PR genes expression and enhancing wheat resistance to Pst.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by-nc-nd. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 8
Claim · model-written summary
Transient silencing of TaASR2L substantially reduced wheat resistance to Pst.
- Taxon
- wheat
Triticum aestivume1 - Treatment
- transient silencing of TaASR2L using a virus-induced gene silencing systeme1
- Interactor
- Pste1pathogen
- Gene
- TaASR2Le1
- Outcome
- resistancee1
- Direction
- reducede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“transient silencing of TaASR2L using a virus-induced gene silencing system substantially reduced wheat resistance to Pst”
Claim · model-written summary
TaASR2L was validated to interact with TaSR30, mainly in the nucleus.
- Taxon
- wheatTriticum aestivume0
- Gene
- TaASR2Le2
- Outcome
- interactione2
- Qualifier
- mainly in the nucleuse2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“is one of the most destructive wheat diseases”
e2“TaASR2L interaction with serine/arginine-rich splicing factor SR30-like (TaSR30) was validated mainly in the nucleus”
Claim · model-written summary
Knockdown of TaSR30 substantially reduced wheat resistance to Pst.
- Taxon
- wheatTriticum aestivume0
- Treatment
- Knockdown of TaSR30 expressione3
- Interactor
- Pste3pathogen
- Gene
- TaSR30e3
- Outcome
- resistancee3
- Direction
- reducede3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“is one of the most destructive wheat diseases”
e3“Knockdown of TaSR30 expression substantially reduced wheat resistance to Pst”
Claim · model-written summary
Overexpression of TaASR2L and TaSR30 promoted expression of ABA- and resistance-related genes.
- Taxon
- wheatTriticum aestivume4
- Treatment
- Overexpression of TaASR2L and TaSR30e4
- Gene
- TaSR30e4
- Outcome
- expression of ABA- and resistance-related genese4
- Direction
- promotee4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e4“Overexpression of TaASR2L and TaSR30 demonstrated that they can promote the expression of ABA- and resistance-related genes to enhance wheat resistance to Pst”
Claim · model-written summary
Exogenous ABA substantially increased the expression levels of TaSR30 and TaASR2L in wheat.
- Taxon
- wheatTriticum aestivume5
- Treatment
- exogenous ABAe5
- Gene
- TaSR30e5
- Outcome
- expression levels of TaSR30 and TaASR2Le5
- Direction
- substantially increasede5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e5“the expression levels of TaSR30 and TaASR2L were substantially increased by exogenous ABA, and the resistance of wheat to Pst was increased, and the expression of PR genes was induced”
Claim · model-written summary
Exogenous ABA increased wheat resistance to Pst.
- Taxon
- wheatTriticum aestivume5
- Treatment
- exogenous ABAe5
- Interactor
- Pste5pathogen
- Outcome
- resistance of wheat to Pste5
- Direction
- was increasede5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e5“the expression levels of TaSR30 and TaASR2L were substantially increased by exogenous ABA, and the resistance of wheat to Pst was increased, and the expression of PR genes was induced”
Claim · model-written summary
Exogenous ABA induced the expression of PR genes in wheat.
- Taxon
- wheatTriticum aestivume5
- Treatment
- exogenous ABAe5
- Outcome
- expression of PR genese5
- Direction
- inducede5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e5“the expression levels of TaSR30 and TaASR2L were substantially increased by exogenous ABA, and the resistance of wheat to Pst was increased, and the expression of PR genes was induced”
Claim · model-written summary
TaASR2L interacts with TaSR30 and promotes PR gene expression, enhancing wheat resistance to Pst.
- Taxon
- wheatTriticum aestivume6
- Gene
- TaASR2Le6
- Outcome
- resistance to Pste6
- Qualifier
- promoting PR genes expressione6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e6“Therefore, these results suggest that TaASR2L interacts with TaSR30 by promoting PR genes expression and enhancing wheat resistance to Pst”
The same questions in other papers
(+)-abscisic acid · quality: protein · Triticum aestivum (wheat)Positive and negative results
5 papers in the same direction as this paper · 2 in the opposite direction
- negative
- negative
- positive
4 papers more in this group
See all 8 papers →(+)-abscisic acid · disease · Triticum aestivum (wheat)Positive and negative results
7 papers in the same direction as this paper · 3 in the opposite direction
- negative
- negative
- negative
7 papers more in this group
See all 11 papers →(+)-abscisic acid · molecular: expression · Triticum aestivum (wheat)Positive and negative results
33 papers in the same direction as this paper · 9 in the opposite direction
- positive
- negative
- negative
39 papers more in this group
See all 43 papers →loss of function · disease · Triticum aestivum (wheat)Positive and negative results
95 papers in the same direction as this paper · 81 in the opposite direction
- positive
- no effect
- negative
173 papers more in this group
See all 177 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.