Abstract
NAC family transcription factors (TFs) are important regulators in plant development and stress responses. However, the biological functions of NAC TFs in wheat are rarely studied. In this study, 43 putative drought-induced NAC genes were identified from de novo transcriptome sequencing data of wheat following drought treatment. Twelve wheat NACs along with ten known stress-related NACs from Arabidopsis and rice were clustered into Group II based on a phylogenetic analysis. TaNAC48, which showed a higher and constitutive expression level in Group II, was selected for further investigation. TaNAC48 transcript was up-regulated by drought, PEG, H2O2 and abscisic acid (ABA) treatment and encoded a nuclear localized protein. Overexpression of TaNAC48 significantly promoted drought tolerance with increased proline content, and decreased rates of water loss, malondialdehyde (MDA), H2O2 and O2− content. Root length and a stomatal aperture assay confirmed that TaNAC48-overexpression plants increased sensitivity to ABA. Electrophoretic mobility shift assay (EMSA) and luciferase reporter analysis indicated that TaAREB3 could bind to a cis-acting ABA-responsive element (ABRE) on TaNAC48 promoter and activate the expression of TaNAC48. These results suggest that TaNAC48 is essential in mediating crosstalk between the ABA signaling pathway and drought stress responses in wheat.
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Claims 18
Claim · model-written summary
Forty-three putative drought-induced NAC genes were identified in wheat transcriptome data.
- Taxon
- wheat
Triticum aestivume0 - Treatment
- drought treatmente0
- Outcome
- 43 putative drought-induced NAC genese0
- Value
- 43e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“43 putative drought-induced NAC genes were identified from de novo transcriptome sequencing data of wheat following drought treatment”
Claim · model-written summary
Twelve wheat NACs clustered in Group II with ten stress-related NACs from Arabidopsis and rice.
- Taxon
- wheatTriticum aestivume0
- Outcome
- clustered into Group IIe0
- Value
- Twelvee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Twelve wheat NACs along with ten known stress-related NACs from Arabidopsis and rice were clustered into Group II based on a phylogenetic analysis”
Claim · model-written summary
TaNAC48 showed higher constitutive expression in Group II.
- Taxon
- wheatTriticum aestivumt0
- Gene
- TaNAC48e0
- Outcome
- expression levele0
- Direction
- highere0
- Comparator
- Group IIe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“TaNAC48, which showed a higher and constitutive expression level in Group II”
t0“wheat (Triticum aestivum L.)”
Claim · model-written summary
TaNAC48 transcript was up-regulated by drought.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- droughte0
- Gene
- TaNAC48e0
- Outcome
- TaNAC48 transcripte0
- Direction
- up-regulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“TaNAC48 transcript was up-regulated by drought, PEG, H2O2 and abscisic acid (ABA) treatment”
t0“wheat (Triticum aestivum L.)”
Claim · model-written summary
TaNAC48 transcript was up-regulated by PEG.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- PEGe0
- Gene
- TaNAC48e0
- Outcome
- TaNAC48 transcripte0
- Direction
- up-regulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“TaNAC48 transcript was up-regulated by drought, PEG, H2O2 and abscisic acid (ABA) treatment”
t0“wheat (Triticum aestivum L.)”
Claim · model-written summary
TaNAC48 transcript was up-regulated by H2O2.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- H2O2e0
- Gene
- TaNAC48e0
- Outcome
- TaNAC48 transcripte0
- Direction
- up-regulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“TaNAC48 transcript was up-regulated by drought, PEG, H2O2 and abscisic acid (ABA) treatment”
t0“wheat (Triticum aestivum L.)”
Claim · model-written summary
TaNAC48 transcript was up-regulated by abscisic acid (ABA).
- Taxon
- wheatTriticum aestivumt0
- Treatment
- abscisic acid (ABA)e0
- Gene
- TaNAC48e0
- Outcome
- TaNAC48 transcripte0
- Direction
- up-regulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“TaNAC48 transcript was up-regulated by drought, PEG, H2O2 and abscisic acid (ABA) treatment”
t0“wheat (Triticum aestivum L.)”
Claim · model-written summary
TaNAC48 encoded a nuclear localized protein.
- Taxon
- wheatTriticum aestivumt0
- Gene
- TaNAC48t0
- Outcome
- nuclear localized proteine0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“encoded a nuclear localized protein”
t0“TaNAC48 positively regulates drought tolerance and ABA responses in wheat (Triticum aestivum L.)”
Claim · model-written summary
TaNAC48 overexpression promoted drought tolerance under drought.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- Overexpression of TaNAC48e0
- Gene
- TaNAC48e0
- Outcome
- drought tolerancee0
- Direction
- promotede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Overexpression of TaNAC48 significantly promoted drought tolerance with increased proline content, and decreased rates of water loss, malondialdehyde (MDA), H2O2 and O2− content”
t0“wheat (Triticum aestivum L.)”
Claim · model-written summary
TaNAC48 overexpression increased proline content under drought.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- Overexpression of TaNAC48e0
- Gene
- TaNAC48e0
- Outcome
- proline contente0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Overexpression of TaNAC48 significantly promoted drought tolerance with increased proline content, and decreased rates of water loss, malondialdehyde (MDA), H2O2 and O2− content”
t0“wheat (Triticum aestivum L.)”
Claim · model-written summary
TaNAC48 overexpression decreased rates of water loss under drought.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- Overexpression of TaNAC48e0
- Gene
- TaNAC48e0
- Outcome
- rates of water losse0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Overexpression of TaNAC48 significantly promoted drought tolerance with increased proline content, and decreased rates of water loss, malondialdehyde (MDA), H2O2 and O2− content”
t0“wheat (Triticum aestivum L.)”
Claim · model-written summary
TaNAC48 overexpression decreased malondialdehyde (MDA) under drought.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- Overexpression of TaNAC48e0
- Gene
- TaNAC48e0
- Outcome
- malondialdehyde (MDA)e0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Overexpression of TaNAC48 significantly promoted drought tolerance with increased proline content, and decreased rates of water loss, malondialdehyde (MDA), H2O2 and O2− content”
t0“wheat (Triticum aestivum L.)”
Claim · model-written summary
TaNAC48 overexpression decreased H2O2 under drought.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- Overexpression of TaNAC48e0
- Gene
- TaNAC48e0
- Outcome
- H2O2e0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Overexpression of TaNAC48 significantly promoted drought tolerance with increased proline content, and decreased rates of water loss, malondialdehyde (MDA), H2O2 and O2− content”
t0“wheat (Triticum aestivum L.)”
Claim · model-written summary
TaNAC48 overexpression decreased O2− content under drought.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- Overexpression of TaNAC48e0
- Gene
- TaNAC48e0
- Outcome
- O2− contente0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Overexpression of TaNAC48 significantly promoted drought tolerance with increased proline content, and decreased rates of water loss, malondialdehyde (MDA), H2O2 and O2− content”
t0“wheat (Triticum aestivum L.)”
Claim · model-written summary
TaNAC48 overexpression increased ABA sensitivity in root length and stomatal aperture assays.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- TaNAC48-overexpressione0
- Gene
- TaNAC48e0
- Outcome
- sensitivity to ABAe0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Root length and a stomatal aperture assay confirmed that TaNAC48-overexpression plants increased sensitivity to ABA”
t0“wheat (Triticum aestivum L.)”
Claim · model-written summary
TaAREB3 bound an ABRE on the TaNAC48 promoter.
- Taxon
- wheatTriticum aestivumt0
- Gene
- TaAREB3e0
- Outcome
- bind to a cis-acting ABA-responsive element (ABRE) on TaNAC48 promotere0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“TaAREB3 could bind to a cis-acting ABA-responsive element (ABRE) on TaNAC48 promoter”
t0“wheat (Triticum aestivum L.)”
Claim · model-written summary
TaAREB3 activated TaNAC48 expression.
- Taxon
- wheatTriticum aestivumt0
- Gene
- TaAREB3e0
- Outcome
- expression of TaNAC48e0
- Direction
- activatee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“TaAREB3 could bind to a cis-acting ABA-responsive element (ABRE) on TaNAC48 promoter and activate the expression of TaNAC48”
t0“wheat (Triticum aestivum L.)”
Claim · model-written summary
The authors suggest TaNAC48 mediates crosstalk between ABA signaling and drought responses in wheat.
- Taxon
- wheatTriticum aestivume0
- Gene
- TaNAC48e0
- Outcome
- crosstalk between the ABA signaling pathway and drought stress responsese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“TaNAC48 is essential in mediating crosstalk between the ABA signaling pathway and drought stress responses in wheat”
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