Paper · 2019 · Archives of Agronomy and Soil Science

Inoculation of diazotrophic bacteria modifies the growth rate and grain yield of maize at different levels of nitrogen supply

Farley Alexandre da Fonseca Breda; Gabriela Cavalcanti Alves; Bruna Daniela Ortiz Lopez; et al.

8
claims
26
verbatim quotes
16
citations
3
research areas
—
Not open access

Abstract

This work intended to evaluate the contribution of single inoculation of diazotrophic bacteria Herbaspirillum seropedicae or Azospirillum brasilense on growth and yield of maize crop at different levels of N fertilization. Two field experiments were carried out in the summer and winter seasons. Factorial design combined four fertilization levels (0, 50, 100 and 150 kg N ha−1) and three seed inoculations (non-inoculation, strains Hs-ZAE94 or Ab-Sp245), with four replications. Shoot dry matter, leaf area index, and N accumulation were evaluated in weekly samplings, and grain yield measured at maturity. Most expressive responses of inoculation were observed in the summer crop, with minor effects in the winter crop when growth and yield were lower. In the summer, inoculation increased shoot dry matter and leaf area of maize plants, enhancing crop growth rate, and increased N accumulated by shoots, particularly at low levels of N supply. In the summer, Hs-ZAE94 increased grain yield at the levels of 0, 50 and 100 kg N ha−1, and Ab-Sp245 at 100 kg N ha−1, as compared to non-inoculation. Inoculation with Hs-ZAE94 associated with 50 kg N ha−1 provided similar grain yield than the non-inoculation with 150 kg N ha−1, improving N use efficiency.

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Claims 8

Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Inoculation increased shoot dry matter and leaf area of maize in summer compared with non-inoculation.

Taxon
maizeZea mayst0
Treatment
inoculatione1
Interactor
diazotrophic bacteriat1symbiont
Outcome
shoot dry matter and leaf areae1
Direction
increasede1
Comparator
non-inoculatione5
Setting
summere1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

t0

“maize”

✓ located verbatim in the source · title, characters 81–86 · show in text ↑
t1

“Inoculation of diazotrophic bacteria”

✓ located verbatim in the source · title, characters 0–36 · show in text ↑
e1

“In the summer, inoculation increased shoot dry matter and leaf area of maize plants, enhancing crop growth rate, and increased N accumulated by shoots, particularly at low levels of N supply”

✓ located verbatim in the source · abstract, characters 755–945 · show in text ↑
e5

“as compared to non-inoculation”

✓ located verbatim in the source · abstract, characters 1066–1096 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Inoculation enhanced crop growth rate of maize in summer compared with non-inoculation.

Taxon
maizeZea mayst0
Treatment
inoculatione1
Interactor
diazotrophic bacteriat1symbiont
Outcome
crop growth ratee1
Direction
enhancinge1
Comparator
non-inoculatione5
Setting
summere1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

t0

“maize”

✓ located verbatim in the source · title, characters 81–86 · show in text ↑
t1

“Inoculation of diazotrophic bacteria”

✓ located verbatim in the source · title, characters 0–36 · show in text ↑
e1

“In the summer, inoculation increased shoot dry matter and leaf area of maize plants, enhancing crop growth rate, and increased N accumulated by shoots, particularly at low levels of N supply”

✓ located verbatim in the source · abstract, characters 755–945 · show in text ↑
e5

“as compared to non-inoculation”

✓ located verbatim in the source · abstract, characters 1066–1096 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Inoculation increased N accumulated by maize shoots in summer, particularly at low N supply, compared with non-inoculation.

Taxon
maizeZea mayst0
Treatment
inoculatione1
Interactor
diazotrophic bacteriat1symbiont
Outcome
N accumulated by shootse1
Direction
increasede1
Qualifier
particularly at low levels of N supplye1
Comparator
non-inoculatione5
Setting
summere1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

t0

“maize”

✓ located verbatim in the source · title, characters 81–86 · show in text ↑
t1

“Inoculation of diazotrophic bacteria”

✓ located verbatim in the source · title, characters 0–36 · show in text ↑
e1

“In the summer, inoculation increased shoot dry matter and leaf area of maize plants, enhancing crop growth rate, and increased N accumulated by shoots, particularly at low levels of N supply”

✓ located verbatim in the source · abstract, characters 755–945 · show in text ↑
e5

“as compared to non-inoculation”

✓ located verbatim in the source · abstract, characters 1066–1096 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Hs-ZAE94 increased maize grain yield at 0, 50 and 100 kg N ha−1 compared with non-inoculation.

Taxon
maizeZea mayst0
Treatment
Hs-ZAE94e2
Interactor
Herbaspirillum seropedicaeHerbaspirillum seropedicaee0symbiont
Outcome
grain yielde2
Direction
increasede2
Qualifier
0, 50 and 100 kg N ha−1e2
Comparator
non-inoculatione2
Setting
summere2

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

t0

“maize”

✓ located verbatim in the source · title, characters 81–86 · show in text ↑
e2

“In the summer, Hs-ZAE94 increased grain yield at the levels of 0, 50 and 100 kg N ha−1, and Ab-Sp245 at 100 kg N ha−1, as compared to non-inoculation”

✓ located verbatim in the source · abstract, characters 947–1096 · show in text ↑
e0

“single inoculation of diazotrophic bacteria Herbaspirillum seropedicae or Azospirillum brasilense”

✓ located verbatim in the source · abstract, characters 51–148 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Ab-Sp245 increased maize grain yield at 100 kg N ha−1 compared with non-inoculation.

Taxon
maizeZea mayst0
Treatment
Ab-Sp245e2
Interactor
Azospirillum brasilenseAzospirillum brasilensee0symbiont
Outcome
grain yielde2
Direction
increasede2
Qualifier
100 kg N ha−1e2
Comparator
non-inoculatione2
Setting
summere2

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗
Azospirillum brasilense
Azospirillum brasilense · InteractorCristiane Colodel · CC BY 4.0 · iNaturalist ↗

Verbatim

t0

“maize”

✓ located verbatim in the source · title, characters 81–86 · show in text ↑
e2

“In the summer, Hs-ZAE94 increased grain yield at the levels of 0, 50 and 100 kg N ha−1, and Ab-Sp245 at 100 kg N ha−1, as compared to non-inoculation”

✓ located verbatim in the source · abstract, characters 947–1096 · show in text ↑
e0

“single inoculation of diazotrophic bacteria Herbaspirillum seropedicae or Azospirillum brasilense”

✓ located verbatim in the source · abstract, characters 51–148 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

Inoculation with Hs-ZAE94 at 50 kg N ha−1 gave grain yield similar to non-inoculation at 150 kg N ha−1.

Taxon
maizeZea mayst0
Treatment
Inoculation with Hs-ZAE94 associated with 50 kg N ha−1e3
Interactor
Herbaspirillum seropedicaeHerbaspirillum seropedicaee0symbiont
Outcome
grain yielde3
Direction
similare3
Comparator
non-inoculation with 150 kg N ha−1e3

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

t0

“maize”

✓ located verbatim in the source · title, characters 81–86 · show in text ↑
e3

“Inoculation with Hs-ZAE94 associated with 50 kg N ha−1 provided similar grain yield than the non-inoculation with 150 kg N ha−1, improving N use efficiency”

✓ located verbatim in the source · abstract, characters 1098–1253 · show in text ↑
e0

“single inoculation of diazotrophic bacteria Herbaspirillum seropedicae or Azospirillum brasilense”

✓ located verbatim in the source · abstract, characters 51–148 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Inoculation with Hs-ZAE94 at 50 kg N ha−1 improved N use efficiency relative to non-inoculation at 150 kg N ha−1.

Taxon
maizeZea mayst0
Treatment
Inoculation with Hs-ZAE94 associated with 50 kg N ha−1e3
Interactor
Herbaspirillum seropedicaeHerbaspirillum seropedicaee0symbiont
Outcome
N use efficiencye3
Direction
improvinge3
Comparator
non-inoculation with 150 kg N ha−1e3

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

t0

“maize”

✓ located verbatim in the source · title, characters 81–86 · show in text ↑
e3

“Inoculation with Hs-ZAE94 associated with 50 kg N ha−1 provided similar grain yield than the non-inoculation with 150 kg N ha−1, improving N use efficiency”

✓ located verbatim in the source · abstract, characters 1098–1253 · show in text ↑
e0

“single inoculation of diazotrophic bacteria Herbaspirillum seropedicae or Azospirillum brasilense”

✓ located verbatim in the source · abstract, characters 51–148 · show in text ↑
Associationobservedoriginal resultpositivemeasured

Claim · model-written summary

Responses to inoculation were most expressive in the summer crop, with minor effects in the winter crop.

Taxon
maizeZea mayst0
Outcome
responses of inoculatione4
Direction
Most expressivee4
Comparator
winter crope4
Setting
summer crope4

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

t0

“maize”

✓ located verbatim in the source · title, characters 81–86 · show in text ↑
e4

“Most expressive responses of inoculation were observed in the summer crop, with minor effects in the winter crop when growth and yield were lower”

✓ located verbatim in the source · abstract, characters 608–753 · show in text ↑

inoculation · growth · Zea mays (maize)Positive and negative results

699 papers: 635 positive, 64 negative or no effect

634 papers in the same direction as this paper · 61 in the opposite direction · 3 reporting no difference from an alternative

  1. positive
  2. positive
  3. negative

695 papers more in this group

See all 699 papers →

nitrogen · Herbaspirillum seropedicae · Zea mays (maize)Results agree

3 papers: 3 positive, 0 negative or no effect

2 papers in the same direction as this paper

  1. positive
  2. positive
See all 3 papers →

inoculation · Herbaspirillum seropedicae · Zea mays (maize)Results agree

8 papers: 8 positive, 0 negative or no effect

7 papers in the same direction as this paper

  1. positive
  2. positive
  3. positive

4 papers more in this group

See all 8 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.