Abstract
We have earlier reported the effects of chemically synthesized copper nanoparticles (CuNPs) on oxalate oxidase (OxOx) activity, extracted from the shoot tissue of germinating grain sorghum i.e. in vitro . Here, we tried to study this effect in vivo and compare it with those of Cu2+. We describe herein, characterization of CuNPs and their effects on oxalate oxidation system i.e. OxOx activity, total oxalate and H2O2 content in vivo i.e. in shoot tissues/leaves of germinating grain Sorghum ( Sorghum vulgare L). To achieve it, grain sorghum seeds were grown up to 10 days in laboratory, irrigated with Hoagland’s solution containing either CuNPs (1.0 ppm) or Cu2+ (1.0 ppm) after 4 days of germination. Control were irrigated with Hoagland solution only. The shoot/leaves of the seedling plants were harvested at 4, 6, 8 and 10 day of germination and analysed quantitatively for OxOx activity, soluble protein, H2O2 and total oxalate. The growth of the Sorghum seedling plants supplemented with CuNPs and Cu2+ was decreased significantly (P <0.1) at all growth stages compared to control. This inhibitory effect of CuNPs was higher than Cu2+. CuNPs decreased the activity of OxOx but Cu2+ had no effect at day 10. Both CuNPs and Cu2+ decreased the specific activity of OxOx and H2O2 content but increased total oxalate content at day 10. The decrease in H2O2 content in both CuNPs and Cu2+ supplemented shoot tissues with concomitant increase in oxalate content confirmed the decreased activity of OxOx in CuNPs and Cu2+ supplemented seedling plants.
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Claims 10
Claim · model-written summary
Growth of sorghum seedlings supplemented with CuNPs was significantly decreased at all growth stages compared with control.
SorghumOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The growth of the Sorghum seedling plants supplemented with CuNPs and Cu2+ was decreased significantly (P <0.1) at all growth stages compared to control”
Claim · model-written summary
Growth of sorghum seedlings supplemented with Cu2+ was significantly decreased at all growth stages compared with control.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The growth of the Sorghum seedling plants supplemented with CuNPs and Cu2+ was decreased significantly (P <0.1) at all growth stages compared to control”
Claim · model-written summary
The inhibitory effect of CuNPs on sorghum seedling growth was higher than that of Cu2+.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“This inhibitory effect of CuNPs was higher than Cu2+”
t0“Sorghum”
Claim · model-written summary
CuNPs decreased oxalate oxidase (OxOx) activity in sorghum seedlings at day 10.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“CuNPs decreased the activity of OxOx but Cu2+ had no effect at day 10”
e1“Control were irrigated with Hoagland solution only”
t0“Sorghum”
Claim · model-written summary
Cu2+ had no effect on oxalate oxidase (OxOx) activity in sorghum seedlings at day 10.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“CuNPs decreased the activity of OxOx but Cu2+ had no effect at day 10”
e1“Control were irrigated with Hoagland solution only”
t0“Sorghum”
Claim · model-written summary
CuNPs decreased the specific activity of OxOx and the H2O2 content in sorghum seedling shoots at day 10.
- Treatment
- CuNPse0
- Outcome
- specific activity of OxOx and H2O2 contente0
- Direction
- decreasede0
- Qualifier
- day 10e0
- Comparator
- Controle1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Both CuNPs and Cu2+ decreased the specific activity of OxOx and H2O2 content but increased total oxalate content at day 10”
e1“Control were irrigated with Hoagland solution only”
t0“Sorghum”
Claim · model-written summary
Cu2+ decreased the specific activity of OxOx and the H2O2 content in sorghum seedling shoots at day 10.
- Treatment
- Cu2+e0
- Outcome
- specific activity of OxOx and H2O2 contente0
- Direction
- decreasede0
- Qualifier
- day 10e0
- Comparator
- Controle1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Both CuNPs and Cu2+ decreased the specific activity of OxOx and H2O2 content but increased total oxalate content at day 10”
e1“Control were irrigated with Hoagland solution only”
t0“Sorghum”
Claim · model-written summary
CuNPs increased total oxalate content in sorghum seedling shoots at day 10.
- Treatment
- CuNPse0
- Outcome
- total oxalate contente0
- Direction
- increasede0
- Qualifier
- day 10e0
- Comparator
- Controle1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Both CuNPs and Cu2+ decreased the specific activity of OxOx and H2O2 content but increased total oxalate content at day 10”
e1“Control were irrigated with Hoagland solution only”
t0“Sorghum”
Claim · model-written summary
Cu2+ increased total oxalate content in sorghum seedling shoots at day 10.
- Treatment
- Cu2+e0
- Outcome
- total oxalate contente0
- Direction
- increasede0
- Qualifier
- day 10e0
- Comparator
- Controle1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Both CuNPs and Cu2+ decreased the specific activity of OxOx and H2O2 content but increased total oxalate content at day 10”
e1“Control were irrigated with Hoagland solution only”
t0“Sorghum”
Claim · model-written summary
The authors interpret the lower H2O2 content together with higher oxalate content as confirming decreased OxOx activity in CuNPs and Cu2+ supplemented seedlings.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The decrease in H2O2 content in both CuNPs and Cu2+ supplemented shoot tissues with concomitant increase in oxalate content confirmed the decreased activity of OxOx in CuNPs and Cu2+ supplemented seedling plants”
t0“Sorghum”