Abstract
Shoot zinc (Zn) concentration in Brassica oleracea is affected by soil Zn and phosphorus (P) supply. Most problematic is the negative impact of P fertilizers on Zn concentrations in crops, which makes balancing yield and mineral quality challenging. To evaluate early molecular mechanisms involved in the accumulation of large shoot Zn concentrations regardless of the P supply, two B. oleracea accessions differing in root architecture and root exudates were grown hydroponically for two weeks with different combinations of P and Zn supply. Ionome profiling and deep RNA sequencing of roots revealed interactions of P and Zn in planta, without apparent phenotypic effects. In addition, increasing P supply did not reduce tissue Zn concentration. Substantial changes in gene expression in response to different P and/ or Zn supplies in roots of both accessions ensured nutritionally sufficient P and Zn uptake. Numerous genes were differentially expressed after changing Zn or P supply and most of them were unique to only one accession, highlighting their different strategies in achieving nutrient sufficiency. Thus, different gene networks responded to the changing P and Zn supply in the two accessions. Additionally, enrichment analysis of gene ontology classes revealed that genes involved in lipid metabolism, response to starvation, and anion transport mechanisms were most responsive to differences in P and Zn supply in both accessions. The results agreed with previously studies demonstrating alterations in P and Zn transport and phospholipid metabolism in response to reduced P and Zn supply. It is anticipated that improved knowledge of genes responsive to P or Zn supply will help illuminate the roles in uptake and accumulation of P and Zn and might identify candidate genes for breeding high-yield-high-Zn brassicas.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 7
Claim · model-written summary
Ionome profiling and RNA sequencing of B. oleracea roots revealed interactions of P and Zn in planta without apparent phenotypic effects.
- Taxon
- Brassica oleracea
Brassica oleraceat0 - Organ / stage
- rootse1
- Outcome
- interactions of P and Zne1
- Qualifier
- without apparent phenotypic effectse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Brassica oleracea Roots to Zinc Supply”
e1“Ionome profiling and deep RNA sequencing of roots revealed interactions of P and Zn in planta, without apparent phenotypic effects”
Claim · model-written summary
Increasing P supply did not reduce tissue Zn concentration in B. oleracea.
- Taxon
- Brassica oleraceaBrassica oleraceat0
- Treatment
- increasing P supplye2
- Outcome
- tissue Zn concentratione2
- Direction
- did not reducee2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Brassica oleracea Roots to Zinc Supply”
e2“increasing P supply did not reduce tissue Zn concentration”
Claim · model-written summary
Different P and/or Zn supplies caused substantial changes in root gene expression in both B. oleracea accessions.
- Taxon
- Brassica oleraceaBrassica oleraceat0
- Material
- both accessionse3
- Organ / stage
- rootse3
- Treatment
- different P and/ or Zn suppliese3
- Outcome
- gene expressione3
- Direction
- Substantial changese3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Brassica oleracea Roots to Zinc Supply”
e3“Substantial changes in gene expression in response to different P and/ or Zn supplies in roots of both accessions ensured nutritionally sufficient P and Zn uptake”
Claim · model-written summary
Changing Zn or P supply altered the expression of numerous genes in B. oleracea roots.
- Taxon
- Brassica oleraceaBrassica oleraceat0
- Treatment
- changing Zn or P supplye4
- Outcome
- genese4
- Direction
- differentially expressede4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Brassica oleracea Roots to Zinc Supply”
e4“Numerous genes were differentially expressed after changing Zn or P supply”
Claim · model-written summary
Most of the differentially expressed genes were unique to one B. oleracea accession.
- Taxon
- Brassica oleraceaBrassica oleraceat0
- Material
- only one accessione5
- Outcome
- unique to only one accessione5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Brassica oleracea Roots to Zinc Supply”
e5“most of them were unique to only one accession, highlighting their different strategies in achieving nutrient sufficiency”
Claim · model-written summary
Different gene networks responded to changing P and Zn supply in the two B. oleracea accessions.
- Taxon
- Brassica oleraceaBrassica oleraceat0
- Material
- two accessionse6
- Treatment
- changing P and Zn supplye6
- Outcome
- gene networkse6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Brassica oleracea Roots to Zinc Supply”
e6“different gene networks responded to the changing P and Zn supply in the two accessions”
Claim · model-written summary
Genes for lipid metabolism, response to starvation and anion transport were most responsive to P and Zn supply differences in both accessions.
- Taxon
- Brassica oleraceaBrassica oleraceat0
- Material
- both accessionse7
- Treatment
- differences in P and Zn supplye7
- Outcome
- genes involved in lipid metabolism, response to starvation, and anion transport mechanismse7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Brassica oleracea Roots to Zinc Supply”
e7“genes involved in lipid metabolism, response to starvation, and anion transport mechanisms were most responsive to differences in P and Zn supply in both accessions”
The same questions in other papers
phosphorus · nutrient: content · Brassica oleracea (wild cabbage)Positive and negative results
2 papers in the same direction as this paper · 17 in the opposite direction
- positive
- positive
- positive
16 papers more in this group
See all 20 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.