Abstract
The improvement of genetic diversity and acceleration of breeding process are the most important tasks in wheat breeding. In vitro biotechnological methods are of practical interest for breeding process. The current paper has considered the effects of genotypes and nutritious medium on the formation of regenerants. The purpose of the research was to study the ability to androgenesis in winter soft wheat anthers and to identify promising winter soft wheat genotypes. The estimation of the ability to androgenesis was carried out among ten winter wheat samples. 8711 anthers were planted on medium of N6 and PII induction; the 190-2 medium was used for regeneration. As a result of the study, it was found that the process of neoplasms occurrence depends both on the variant of the nutrient medium and on the chosen genotype. It has been proven that the most favorable medium for the cultivation of winter soft wheat anthers is medium N6. It was found that the maximum percentage of neoplasms (5.21%) from winter soft wheat anthers was recorded in the sample “595/13”. It was found that the samples “595/13” (9 plants) and “Niva Dona” (6 plants) developed by the FSBSI “Agricultural Research Center “Donskoy” showed the highest responsiveness to the formation of calluses and plants-regenerants. There were obtained 6 green regenerants and 3 albino forms from the pollen callus of the sample “595/13” and 4 green regenerants and 2 albino regenerants from the sample “Niva Dona”.
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Claims 9
Claim · model-written summary
Neoplasm formation depended on nutrient medium and genotype.
- Taxon
- winter soft wheatt0
- Material
- chosen genotypee0
- Treatment
- variant of the nutrient mediume0
- Outcome
- neoplasms occurrencee0
Not reportedDirection
Verbatim
e0“the process of neoplasms occurrence depends both on the variant of the nutrient medium and on the chosen genotype”
t0“winter soft wheat”
Claim · model-written summary
N6 was the most favorable medium for cultivation of winter soft wheat anthers.
- Taxon
- winter soft wheate0
- Treatment
- medium N6e0
- Outcome
- cultivation of winter soft wheat antherse0
- Direction
- most favorablee0
Not reportedComparator
Verbatim
e0“the most favorable medium for the cultivation of winter soft wheat anthers is medium N6”
Claim · model-written summary
Sample 595/13 had the maximum neoplasm percentage, 5.21%.
Verbatim
e0“the maximum percentage of neoplasms (5.21%) from winter soft wheat anthers was recorded in the sample “595/13””
Claim · model-written summary
Sample 595/13 produced nine plants.
Verbatim
e0““595/13” (9 plants)”
t0“winter soft wheat”
Claim · model-written summary
Niva Dona produced six plants.
Verbatim
e0““Niva Dona” (6 plants)”
t0“winter soft wheat”
Claim · model-written summary
Sample 595/13 yielded six green regenerants.
Verbatim
e0“6 green regenerants and 3 albino forms from the pollen callus of the sample “595/13””
t0“winter soft wheat”
Claim · model-written summary
Sample 595/13 yielded three albino forms.
Verbatim
e0“6 green regenerants and 3 albino forms from the pollen callus of the sample “595/13””
t0“winter soft wheat”
Claim · model-written summary
Niva Dona yielded four green regenerants.
Verbatim
e0“4 green regenerants and 2 albino regenerants from the sample “Niva Dona””
t0“winter soft wheat”
Claim · model-written summary
Niva Dona yielded two albino regenerants.
Verbatim
e0“4 green regenerants and 2 albino regenerants from the sample “Niva Dona””
t0“winter soft wheat”
The same questions in other papers
in vitro · organism · Triticum aestivum (wheat)Positive and negative results
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