Paper · 2020 · Pharmacognosy Journal

Qualitative and Quantitative Assay of Hydroxycinnamates of Prunus spinosa L.

Sergey Kondrashev; Nadezhda Nesterova; Alexey Luzin; et al.

6
claims
11
verbatim quotes
11
citations
2
research areas
✓
Open access · cc-by

Abstract

Background: Blackthorn (Prunus spinosa L.) is a plant commonly found in the Russian Federation on the roadsides, forest margins, and meadows. Despite lack of recognition by the official medicine, blackthorn fruits possess antioxidant properties and are used in homeopathic preparations. They may also demonstrate antibacterial and anticancer potential due to hydroxycinnamic acids. The aim of present study was to identify and assay hydroxycinnamates in fruits of P. spinosa cultivated in Moscow Region. Materials and Methods: Fresh and dried fruits of P. spinosa, gathered from plants cultivated in Moscow Region in the harvest maturity stage, were used in the study. Qualitative composition of hydroxycinnamic acids was assessed by high performance liquid chromatography, using reversed phase C18 column. Total hydroxycinnamic acids (THA) content was assessed spectrophotometrically. Results: Similar chromatographic profiles were obtained for both fresh and dried blackthorn fruits, the two most abundant compounds being epicatechin (2.91%) and chicoric acid (2.90%). Fruits gathered in Chekhovsky District had lower content of hydroxycinnamates (0.798 0.89) than those coming from Klinsky District (0.886 0.92). However, the THA content in dried fruits grown in both districts was found to be similar (0.540 0.71 and 0.557 0.74, respectively). Conclusions: It can be concluded that blackthorn fruits can be considered as a source of hydroxycinnamic acids, as both fresh and dried fruits contain at least eleven hydroxycinnamates. It was found that the dried fruits have similar content of hydroxycinnamic acids, independently of their origin. Future research should be aimed at drying method optimization.

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Claims 6

Observationobservedoriginal resultmeasured

Claim · model-written summary

Fresh and dried blackthorn fruits had similar chromatographic profiles.

Taxon
blackthornPrunus spinosae0
Organ / stage
fruitse0
Outcome
Similar chromatographic profilese0

Not reportedDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Prunus spinosa
Prunus spinosa · TaxonMartin Olsson (mnemo on en/sv wikipedia and commons, martin@minimum.se) · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Similar chromatographic profiles were obtained for both fresh and dried blackthorn fruits”

✓ located verbatim in the source · abstract, characters 895–984 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Epicatechin was among the two most abundant compounds at 2.91%.

Taxon
Prunus spinosa L.Prunus spinosat0
Outcome
epicatechine0
Direction
most abundante0
Value
2.91%e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Prunus spinosa
Prunus spinosa · TaxonMartin Olsson (mnemo on en/sv wikipedia and commons, martin@minimum.se) · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“the two most abundant compounds being epicatechin (2.91%) and chicoric acid (2.90%).”

✓ located verbatim in the source · abstract, characters 986–1070 · show in text ↑
t0

“Prunus spinosa L.”

✓ located verbatim in the source · title, characters 59–76 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Chicoric acid was among the two most abundant compounds at 2.90%.

Taxon
Prunus spinosa L.Prunus spinosat0
Outcome
chicoric acide0
Direction
most abundante0
Value
2.90%e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Prunus spinosa
Prunus spinosa · TaxonMartin Olsson (mnemo on en/sv wikipedia and commons, martin@minimum.se) · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“the two most abundant compounds being epicatechin (2.91%) and chicoric acid (2.90%).”

✓ located verbatim in the source · abstract, characters 986–1070 · show in text ↑
t0

“Prunus spinosa L.”

✓ located verbatim in the source · title, characters 59–76 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Chekhovsky District fruits had lower hydroxycinnamate content than Klinsky District fruits.

Taxon
Prunus spinosa L.Prunus spinosat0
Outcome
content of hydroxycinnamatese0
Direction
lowere0
Value
0.798 0.89e0
Comparator
Klinsky Districte0
Setting
Chekhovsky Districte0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Prunus spinosa
Prunus spinosa · TaxonMartin Olsson (mnemo on en/sv wikipedia and commons, martin@minimum.se) · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Fruits gathered in Chekhovsky District had lower content of hydroxycinnamates (0.798 0.89) than those coming from Klinsky District (0.886 0.92).”

✓ located verbatim in the source · abstract, characters 1071–1215 · show in text ↑
t0

“Prunus spinosa L.”

✓ located verbatim in the source · title, characters 59–76 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Dried fruits from the two districts had similar total hydroxycinnamic acid content.

Taxon
Prunus spinosa L.Prunus spinosat0
Organ / stage
dried fruitse0
Outcome
THA contente0
Direction
similare0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Prunus spinosa
Prunus spinosa · TaxonMartin Olsson (mnemo on en/sv wikipedia and commons, martin@minimum.se) · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“the THA content in dried fruits grown in both districts was found to be similar (0.540 0.71 and 0.557 0.74, respectively).”

✓ located verbatim in the source · abstract, characters 1225–1347 · show in text ↑
t0

“Prunus spinosa L.”

✓ located verbatim in the source · title, characters 59–76 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Fresh and dried blackthorn fruits contained at least eleven hydroxycinnamates.

Taxon
Prunus spinosa L.Prunus spinosat0
Outcome
hydroxycinnamatese0
Value
at least elevene0

Not reportedDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Prunus spinosa
Prunus spinosa · TaxonMartin Olsson (mnemo on en/sv wikipedia and commons, martin@minimum.se) · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“both fresh and dried fruits contain at least eleven hydroxycinnamates.”

✓ located verbatim in the source · abstract, characters 1463–1533 · show in text ↑
t0

“Prunus spinosa L.”

✓ located verbatim in the source · title, characters 59–76 · show in text ↑