Paper · 2020 · Brazilian Journal of Biology

Chemical characterization and antimicrobial activity of Campomanesia aurea against three strains of Listeria monocytogenes

L. A. Pacheco; E. M. Ethur; T. Sheibel; et al.

14
claims
33
verbatim quotes
12
citations
2
research areas
✓
Open access · cc-by

Abstract

The essential oil (EO) composition of leaves of Campomanesia aurea was analyzed by GC-MS. The plant was collected in April (EOA) and October (EOO) in São Francisco de Assis/RS. The essential oils were obtained by hydrodistillation in a modified Clevenger apparatus. The yields were 4.44% in April, and 6.15%, in October. Thirty-one compounds were identified in EOA, accounting for 96.87% of the total, and twenty-eight compounds were identified in EOO, accounting for 99.46% of the total. For EOA, the major identified monoterpene was p-cymene (8.33%) and the major identified sesquiterpene was α-cadinol (10.72%), while regarding EOO the major identified monoterpene was repeatedly Terpinolene (3.43%), whereas the major identified sesquiterpene was α-cadinol (12.79%). The antibacterial activity of the essential oil was evaluated against the three Listeria monocytogenes from the American Type Culture Collection (strains ATCC 7644, 19114 and 13932). The essential oil showed a MIC of 10 mg/mL against the strains ATCC 7644 and 19114, with bacteriostatic effect of 20 mg/mL and a MIC of 20 mg/mL for ATCC 13932. Regarding the antibiofilm activity, o EOO showed the best results with an inhibition of 90% in the sessile growth with an applied concentration of 4.0 mg/mL.

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Claims 14

Observationobservedoriginal resultmeasured

Claim · model-written summary

The April essential oil yield of Campomanesia aurea leaves was 4.44%.

Taxon
Campomanesia aureaCampomanesia aureae0
Organ / stage
leavese0
Outcome
yieldse2
Value
4.44%e2
Qualifier
in Aprile2
Setting
São Francisco de Assis/RSe1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Campomanesia aurea
Campomanesia aurea · TaxonEdson Luis Fabro Gasperin · CC BY-SA 4.0 · iNaturalist ↗

Verbatim

e0

“The essential oil (EO) composition of leaves of Campomanesia aurea was analyzed by GC-MS”

✓ located verbatim in the source · abstract, characters 0–88 · show in text ↑
e1

“The plant was collected in April (EOA) and October (EOO) in São Francisco de Assis/RS”

✓ located verbatim in the source · abstract, characters 90–175 · show in text ↑
e2

“The yields were 4.44% in April, and 6.15%, in October”

✓ located verbatim in the source · abstract, characters 266–319 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

The October essential oil yield of Campomanesia aurea leaves was 6.15%.

Taxon
Campomanesia aureaCampomanesia aureae0
Organ / stage
leavese0
Outcome
yieldse2
Value
6.15%e2
Qualifier
in Octobere2
Setting
São Francisco de Assis/RSe1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Campomanesia aurea
Campomanesia aurea · TaxonEdson Luis Fabro Gasperin · CC BY-SA 4.0 · iNaturalist ↗

Verbatim

e0

“The essential oil (EO) composition of leaves of Campomanesia aurea was analyzed by GC-MS”

✓ located verbatim in the source · abstract, characters 0–88 · show in text ↑
e1

“The plant was collected in April (EOA) and October (EOO) in São Francisco de Assis/RS”

✓ located verbatim in the source · abstract, characters 90–175 · show in text ↑
e2

“The yields were 4.44% in April, and 6.15%, in October”

✓ located verbatim in the source · abstract, characters 266–319 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Thirty-one compounds were identified in the April essential oil.

Taxon
Campomanesia aureaCampomanesia aureae0
Outcome
compoundse3
Value
Thirty-one compoundse3
Qualifier
in EOAe3

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Campomanesia aurea
Campomanesia aurea · TaxonEdson Luis Fabro Gasperin · CC BY-SA 4.0 · iNaturalist ↗

Verbatim

e0

“The essential oil (EO) composition of leaves of Campomanesia aurea was analyzed by GC-MS”

✓ located verbatim in the source · abstract, characters 0–88 · show in text ↑
e3

“Thirty-one compounds were identified in EOA, accounting for 96.87% of the total”

✓ located verbatim in the source · abstract, characters 321–400 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Twenty-eight compounds were identified in the October essential oil.

Taxon
Campomanesia aureaCampomanesia aureae0
Outcome
compoundse3
Value
twenty-eight compoundse3
Qualifier
in EOOe3

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Campomanesia aurea
Campomanesia aurea · TaxonEdson Luis Fabro Gasperin · CC BY-SA 4.0 · iNaturalist ↗

Verbatim

e0

“The essential oil (EO) composition of leaves of Campomanesia aurea was analyzed by GC-MS”

✓ located verbatim in the source · abstract, characters 0–88 · show in text ↑
e3

“twenty-eight compounds were identified in EOO, accounting for 99.46% of the total”

✓ located verbatim in the source · abstract, characters 406–487 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

The identified compounds in the April essential oil accounted for 96.87% of the total.

Taxon
Campomanesia aureaCampomanesia aureae0
Outcome
accounting fore3
Value
96.87%e3
Qualifier
of the totale3

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Campomanesia aurea
Campomanesia aurea · TaxonEdson Luis Fabro Gasperin · CC BY-SA 4.0 · iNaturalist ↗

Verbatim

e0

“The essential oil (EO) composition of leaves of Campomanesia aurea was analyzed by GC-MS”

✓ located verbatim in the source · abstract, characters 0–88 · show in text ↑
e3

“Thirty-one compounds were identified in EOA, accounting for 96.87% of the total”

✓ located verbatim in the source · abstract, characters 321–400 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

The identified compounds in the October essential oil accounted for 99.46% of the total.

Taxon
Campomanesia aureaCampomanesia aureae0
Outcome
accounting fore3
Value
99.46%e3
Qualifier
of the totale3

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Campomanesia aurea
Campomanesia aurea · TaxonEdson Luis Fabro Gasperin · CC BY-SA 4.0 · iNaturalist ↗

Verbatim

e0

“The essential oil (EO) composition of leaves of Campomanesia aurea was analyzed by GC-MS”

✓ located verbatim in the source · abstract, characters 0–88 · show in text ↑
e3

“twenty-eight compounds were identified in EOO, accounting for 99.46% of the total”

✓ located verbatim in the source · abstract, characters 406–487 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

p-Cymene was the major identified monoterpene in the April essential oil, at 8.33%.

Taxon
Campomanesia aureaCampomanesia aureae0
Compound
p-cymenee4
Outcome
major identified monoterpenee4
Value
8.33%e4
Qualifier
For EOAe4

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Campomanesia aurea
Campomanesia aurea · TaxonEdson Luis Fabro Gasperin · CC BY-SA 4.0 · iNaturalist ↗

Verbatim

e0

“The essential oil (EO) composition of leaves of Campomanesia aurea was analyzed by GC-MS”

✓ located verbatim in the source · abstract, characters 0–88 · show in text ↑
e4

“For EOA, the major identified monoterpene was p-cymene (8.33%)”

✓ located verbatim in the source · abstract, characters 489–551 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

α-Cadinol was the major identified sesquiterpene in the April essential oil, at 10.72%.

Taxon
Campomanesia aureaCampomanesia aureae0
Compound
α-cadinole4
Outcome
major identified sesquiterpenee4
Value
10.72%e4
Qualifier
For EOAe4

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Campomanesia aurea
Campomanesia aurea · TaxonEdson Luis Fabro Gasperin · CC BY-SA 4.0 · iNaturalist ↗

Verbatim

e0

“The essential oil (EO) composition of leaves of Campomanesia aurea was analyzed by GC-MS”

✓ located verbatim in the source · abstract, characters 0–88 · show in text ↑
e4

“For EOA, the major identified monoterpene was p-cymene (8.33%) and the major identified sesquiterpene was α-cadinol (10.72%)”

✓ located verbatim in the source · abstract, characters 489–613 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Terpinolene was the major identified monoterpene in the October essential oil, at 3.43%.

Taxon
Campomanesia aureaCampomanesia aureae0
Compound
Terpinolenee4
Outcome
major identified monoterpenee4
Value
3.43%e4
Qualifier
regarding EOOe4

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Campomanesia aurea
Campomanesia aurea · TaxonEdson Luis Fabro Gasperin · CC BY-SA 4.0 · iNaturalist ↗

Verbatim

e0

“The essential oil (EO) composition of leaves of Campomanesia aurea was analyzed by GC-MS”

✓ located verbatim in the source · abstract, characters 0–88 · show in text ↑
e4

“while regarding EOO the major identified monoterpene was repeatedly Terpinolene (3.43%)”

✓ located verbatim in the source · abstract, characters 615–702 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

α-Cadinol was the major identified sesquiterpene in the October essential oil, at 12.79%.

Taxon
Campomanesia aureaCampomanesia aureae0
Compound
α-cadinole4
Outcome
major identified sesquiterpenee4
Value
12.79%e4
Qualifier
wherease4

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Campomanesia aurea
Campomanesia aurea · TaxonEdson Luis Fabro Gasperin · CC BY-SA 4.0 · iNaturalist ↗

Verbatim

e0

“The essential oil (EO) composition of leaves of Campomanesia aurea was analyzed by GC-MS”

✓ located verbatim in the source · abstract, characters 0–88 · show in text ↑
e4

“whereas the major identified sesquiterpene was α-cadinol (12.79%)”

✓ located verbatim in the source · abstract, characters 704–769 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

The essential oil had a minimum inhibitory concentration of 10 mg/mL against Listeria monocytogenes strains ATCC 7644 and 19114.

Taxon
Campomanesia aureaCampomanesia aureae0
Treatment
essential oile5
Interactor
Listeria monocytogenesListeria monocytogenese7pathogen
Outcome
MICe5
Value
10 mg/mLe5
Qualifier
against the strains ATCC 7644 and 19114e5

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Campomanesia aurea
Campomanesia aurea · TaxonEdson Luis Fabro Gasperin · CC BY-SA 4.0 · iNaturalist ↗
Listeria monocytogenes
Listeria monocytogenes · InteractorElizabeth White · Public domain · Wikimedia Commons ↗

Verbatim

e0

“The essential oil (EO) composition of leaves of Campomanesia aurea was analyzed by GC-MS”

✓ located verbatim in the source · abstract, characters 0–88 · show in text ↑
e5

“The essential oil showed a MIC of 10 mg/mL against the strains ATCC 7644 and 19114, with bacteriostatic effect of 20 mg/mL and a MIC of 20 mg/mL for ATCC 13932”

✓ located verbatim in the source · abstract, characters 954–1113 · show in text ↑
e7

“three Listeria monocytogenes from the American Type Culture Collection”

✓ located verbatim in the source · abstract, characters 845–915 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

The essential oil had a bacteriostatic effect at 20 mg/mL against Listeria monocytogenes.

Taxon
Campomanesia aureaCampomanesia aureae0
Treatment
essential oile5
Interactor
Listeria monocytogenesListeria monocytogenese7pathogen
Outcome
bacteriostatic effecte5
Value
20 mg/mLe5

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Campomanesia aurea
Campomanesia aurea · TaxonEdson Luis Fabro Gasperin · CC BY-SA 4.0 · iNaturalist ↗
Listeria monocytogenes
Listeria monocytogenes · InteractorElizabeth White · Public domain · Wikimedia Commons ↗

Verbatim

e0

“The essential oil (EO) composition of leaves of Campomanesia aurea was analyzed by GC-MS”

✓ located verbatim in the source · abstract, characters 0–88 · show in text ↑
e5

“The essential oil showed a MIC of 10 mg/mL against the strains ATCC 7644 and 19114, with bacteriostatic effect of 20 mg/mL and a MIC of 20 mg/mL for ATCC 13932”

✓ located verbatim in the source · abstract, characters 954–1113 · show in text ↑
e7

“three Listeria monocytogenes from the American Type Culture Collection”

✓ located verbatim in the source · abstract, characters 845–915 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

The essential oil had a minimum inhibitory concentration of 20 mg/mL against Listeria monocytogenes ATCC 13932.

Taxon
Campomanesia aureaCampomanesia aureae0
Treatment
essential oile5
Interactor
Listeria monocytogenesListeria monocytogenese7pathogen
Outcome
MICe5
Value
20 mg/mLe5
Qualifier
for ATCC 13932e5

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Campomanesia aurea
Campomanesia aurea · TaxonEdson Luis Fabro Gasperin · CC BY-SA 4.0 · iNaturalist ↗
Listeria monocytogenes
Listeria monocytogenes · InteractorElizabeth White · Public domain · Wikimedia Commons ↗

Verbatim

e0

“The essential oil (EO) composition of leaves of Campomanesia aurea was analyzed by GC-MS”

✓ located verbatim in the source · abstract, characters 0–88 · show in text ↑
e5

“The essential oil showed a MIC of 10 mg/mL against the strains ATCC 7644 and 19114, with bacteriostatic effect of 20 mg/mL and a MIC of 20 mg/mL for ATCC 13932”

✓ located verbatim in the source · abstract, characters 954–1113 · show in text ↑
e7

“three Listeria monocytogenes from the American Type Culture Collection”

✓ located verbatim in the source · abstract, characters 845–915 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

The October essential oil (EOO) inhibited sessile growth by 90% at 4.0 mg/mL, the best antibiofilm result reported.

Taxon
Campomanesia aureaCampomanesia aureae0
Treatment
EOOe6
Outcome
sessile growthe6
Direction
inhibitione6
Value
90%e6
Qualifier
applied concentration of 4.0 mg/mLe6

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Campomanesia aurea
Campomanesia aurea · TaxonEdson Luis Fabro Gasperin · CC BY-SA 4.0 · iNaturalist ↗

Verbatim

e0

“The essential oil (EO) composition of leaves of Campomanesia aurea was analyzed by GC-MS”

✓ located verbatim in the source · abstract, characters 0–88 · show in text ↑
e6

“Regarding the antibiofilm activity, o EOO showed the best results with an inhibition of 90% in the sessile growth with an applied concentration of 4.0 mg/mL”

✓ located verbatim in the source · abstract, characters 1115–1271 · show in text ↑