Paper · 2020 · DOAJ (DOAJ: Directory of Open Access Journals)

Screening of native isolates of Trichoderma spp. for ability to control cucumber damping-off caused by Phytophthora drechsleri

Marzieh Soheiliara; Mahyar Sheikholeslami; Hamid Reza Zamanizadeh

10
claims
16
verbatim quotes
0
citations
2
research areas
✓
Open access · cc-by-sa

Abstract

In this study, 41 isolates of the genus Trichoderma including six species of Trichoderma arundinaceum, T. asperellum, T. atroviride, T. harzianum, T. longibrachiatum and T. virens were isolated from soils of cucumber fields in Kermanshah province, and their efficacy to control Phytophthora drechsleri, the causal agent of cucumber damping off, was investigated in laboratory and greenhouse conditions. In direct confrontation between Trichoderma isolates and the pathogen, all isolates were able to promote, establish, and sporulate on pathogen mycelia. Through dual culture test, the most inhibitory effect on pathogen growth (62.89%) was recorded for T. harzianum (isolate T1). Volatile compounds of T. harzianum (isolate T7) had the greatest mycelial growth inhibition (46.59%) compared with control. Complete growth inhibition of the pathogen was recorded when the growth medium was supplemented with culture filtrate of Trichoderma isolates at concentration of 15% and 30%. At the concentrations of 15% and 30%, 18 isolates and 22 isolates completely inhibited the pathogen expansion respectively. In the greenhouse, the results of data analysis on the survival percent of plants in various treatments showed significant differences between the isolates of Trichoderma in terms of preventing cucumber damping off. In conclusion, T. asperellum showed the highest ability to control Phytophthora damping off and promoting cucumber growth which is a new record for Iran. This species can be an appropriate choice for biological control of the disease caused by P. drechsleri in cucumber.

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Claims 10

Observationobservedoriginal resultmeasured

Claim · model-written summary

All Trichoderma isolates established and sporulated on pathogen mycelia in confrontation tests.

Taxon
Trichoderma isolatese0
Interactor
pathogene0pathogen
Outcome
establish, and sporulate on pathogen myceliae0

Verbatim

e0

“In direct confrontation between Trichoderma isolates and the pathogen, all isolates were able to promote, establish, and sporulate on pathogen mycelia.”

✓ located verbatim in the source · abstract, characters 403–554 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

T. harzianum isolate T1 had the greatest inhibitory effect on pathogen growth, 62.89%, in dual culture.

Taxon
T. harzianumTrichoderma harzianume0
Treatment
T. harzianum (isolate T1)e0
Interactor
Phytophthora drechsleriPhytophthora drechslerit0pathogen
Outcome
pathogen growthe0
Direction
most inhibitorye0
Value
62.89%e0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Trichoderma harzianum
Trichoderma harzianum · TaxonUS Department of Agriculture, Agricultural Research Service, Systematic Botany and Mycology Laboratory, [1] · Public domain · Wikimedia Commons ↗

Verbatim

e0

“Through dual culture test, the most inhibitory effect on pathogen growth (62.89%) was recorded for T. harzianum (isolate T1).”

✓ located verbatim in the source · abstract, characters 555–680 · show in text ↑
t0

“Phytophthora drechsleri”

✓ located verbatim in the source · title, characters 103–126 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Volatiles of T. harzianum isolate T7 inhibited pathogen mycelial growth by 46.59% relative to control.

Taxon
T. harzianumTrichoderma harzianume0
Treatment
Volatile compounds of T. harzianum (isolate T7)e0
Interactor
Phytophthora drechsleriPhytophthora drechslerit0pathogen
Outcome
mycelial growth inhibitione0
Direction
inhibitione0
Value
46.59%e0
Comparator
controle0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Trichoderma harzianum
Trichoderma harzianum · TaxonUS Department of Agriculture, Agricultural Research Service, Systematic Botany and Mycology Laboratory, [1] · Public domain · Wikimedia Commons ↗

Verbatim

e0

“Volatile compounds of T. harzianum (isolate T7) had the greatest mycelial growth inhibition (46.59%) compared with control.”

✓ located verbatim in the source · abstract, characters 681–804 · show in text ↑
t0

“Phytophthora drechsleri”

✓ located verbatim in the source · title, characters 103–126 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Trichoderma culture filtrate at 15% and 30% completely inhibited pathogen growth.

Taxon
Trichoderma isolatese0
Treatment
culture filtrate of Trichoderma isolates at concentration of 15% and 30%e0
Interactor
Phytophthora drechsleriPhytophthora drechslerit0pathogen
Outcome
growth inhibition of the pathogene0
Direction
Complete growth inhibitione0

Not reportedComparator

Verbatim

e0

“Complete growth inhibition of the pathogen was recorded when the growth medium was supplemented with culture filtrate of Trichoderma isolates at concentration of 15% and 30%.”

✓ located verbatim in the source · abstract, characters 805–979 · show in text ↑
t0

“Phytophthora drechsleri”

✓ located verbatim in the source · title, characters 103–126 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

At 15% filtrate, 18 isolates completely inhibited pathogen expansion.

Taxon
Trichoderma spp.Trichodermat0
Interactor
Phytophthora drechsleriPhytophthora drechslerit0pathogen
Outcome
pathogen expansione0
Value
18 isolatese0
Qualifier
15%e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Trichoderma
Trichoderma · Taxon · picture of the genus (one of its species)US Department of Agriculture, Agricultural Research Service, Systematic Botany and Mycology Laboratory, [1] · Public domain · Wikimedia Commons ↗

Verbatim

e0

“At the concentrations of 15% and 30%, 18 isolates and 22 isolates completely inhibited the pathogen expansion respectively.”

✓ located verbatim in the source · abstract, characters 980–1103 · show in text ↑
t0

“Trichoderma spp. for ability to control cucumber damping-off caused by Phytophthora drechsleri”

✓ located verbatim in the source · title, characters 32–126 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

At 30% filtrate, 22 isolates completely inhibited pathogen expansion.

Taxon
Trichoderma spp.Trichodermat0
Interactor
Phytophthora drechsleriPhytophthora drechslerit0pathogen
Outcome
pathogen expansione0
Value
22 isolatese0
Qualifier
30%e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Trichoderma
Trichoderma · Taxon · picture of the genus (one of its species)US Department of Agriculture, Agricultural Research Service, Systematic Botany and Mycology Laboratory, [1] · Public domain · Wikimedia Commons ↗

Verbatim

e0

“At the concentrations of 15% and 30%, 18 isolates and 22 isolates completely inhibited the pathogen expansion respectively.”

✓ located verbatim in the source · abstract, characters 980–1103 · show in text ↑
t0

“Trichoderma spp. for ability to control cucumber damping-off caused by Phytophthora drechsleri”

✓ located verbatim in the source · title, characters 32–126 · show in text ↑
Intervention effectobservedoriginal resultsignificant (as stated)measured

Claim · model-written summary

Cucumber survival differed significantly among Trichoderma isolate treatments in the greenhouse.

Taxon
cucumberCucumis sativust0
Treatment
isolates of Trichodermae0
Interactor
TrichodermaTrichodermae0biocontrol_agent
Outcome
survival percent of plantse0
Setting
greenhousee0

Not reportedComparatorDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cucumis sativus
Cucumis sativus · TaxonH. Zell · CC BY-SA 3.0 · Wikimedia Commons ↗
Trichoderma
Trichoderma · Interactor · picture of the genus (one of its species)US Department of Agriculture, Agricultural Research Service, Systematic Botany and Mycology Laboratory, [1] · Public domain · Wikimedia Commons ↗

Verbatim

e0

“In the greenhouse, the results of data analysis on the survival percent of plants in various treatments showed significant differences between the isolates of Trichoderma in terms of preventing cucumber damping off.”

✓ located verbatim in the source · abstract, characters 1104–1319 · show in text ↑
t0

“cucumber damping-off”

✓ located verbatim in the source · title, characters 72–92 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

T. asperellum had the highest reported ability to control cucumber Phytophthora damping off.

Taxon
cucumberCucumis sativuse0
Treatment
T. asperellume0
Interactor
PhytophthoraPhytophthorae0pathogen
Second interactor
T. asperellumTrichoderma asperellume0biocontrol_agent
Outcome
control Phytophthora damping offe0
Direction
higheste0
Setting
Irane0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cucumis sativus
Cucumis sativus · TaxonH. Zell · CC BY-SA 3.0 · Wikimedia Commons ↗
Phytophthora
Phytophthora · Interactor · picture of the genus (one of its species)Rasbak · CC BY-SA 3.0 · Wikimedia Commons ↗
Trichoderma asperellum
Trichoderma asperellum · Second interactorTashkoskip · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“In conclusion, T. asperellum showed the highest ability to control Phytophthora damping off and promoting cucumber growth which is a new record for Iran.”

✓ located verbatim in the source · abstract, characters 1320–1473 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

T. asperellum promoted cucumber growth.

Taxon
cucumberCucumis sativuse0
Treatment
T. asperellume0
Interactor
T. asperellumTrichoderma asperellume0biocontrol_agent
Outcome
cucumber growthe0
Direction
promotinge0
Setting
Irane0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cucumis sativus
Cucumis sativus · TaxonH. Zell · CC BY-SA 3.0 · Wikimedia Commons ↗
Trichoderma asperellum
Trichoderma asperellum · InteractorTashkoskip · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“In conclusion, T. asperellum showed the highest ability to control Phytophthora damping off and promoting cucumber growth which is a new record for Iran.”

✓ located verbatim in the source · abstract, characters 1320–1473 · show in text ↑
Recommendationauthor interpretationoriginal resultbasis n/a

Claim · model-written summary

The authors recommended T. asperellum for biological control of cucumber disease caused by P. drechsleri.

Taxon
cucumberCucumis sativuse0
Interactor
P. drechsleriPhytophthora drechslerie0pathogen
Outcome
biological control of the disease caused by P. drechslerie0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cucumis sativus
Cucumis sativus · TaxonH. Zell · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“This species can be an appropriate choice for biological control of the disease caused by P. drechsleri in cucumber.”

✓ located verbatim in the source · abstract, characters 1474–1590 · show in text ↑

Trichoderma asperellum · growth · Cucumis sativus (cucumber)Results agree

2 papers: 2 positive, 0 negative or no effect

1 paper in the same direction as this paper

  1. positive
See all 2 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.