Abstract
This study describes an efficient and widely applicable droplet-vitrification following cryopreservation for shoot tips of strawberry (Fragaria × ananassa Duch.) cvs. ‘Wonkyo3114’ and ‘Gurumi40’. The shoot tips were precultured in Murashige and Skoog (MS) liquid medium supplemented with sucrose (0.3-0.5M). Precultured explants were osmoprotected with loading solution (LS, C4) containing 20% glycerol and 20% sucrose for 40 min and exposed to dehydration solution (B5) containing 40% glycerol and 40% sucrose for 40 min at 25℃, Subsequently, the explants were transferred onto droplets containing 2.5 μL PVS3 on sterilized aluminum foils (4 ㎝ × 0.5 ㎝) prior to direct immersion in liquid nitrogen (LN) for 1 h. The highest regrowth rate (%) in both the cultivars was obtained when the shoot tips were precultured with MS + 0.3M sucrose for 40 h at 25℃. The cryopreserved shoots tips exhibited 55% regrowth rate by culturing in NH4NO3-free MS medium supplemented with 3% sucrose, 1.0 g/L casein, 1.0㎎/L GA3, and 0.5 ㎎/L BA for 5 weeks and in MS medium supplemented with 0.5 ㎎/L GA3 for 8 weeks. This result shows that droplet-vitrification could be employed as a promising method for cryostorage of strawberry germplasm.
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Claims 3
Claim · model-written summary
MS plus 0.3M sucrose for 40 h at 25℃ produced the highest shoot-tip regrowth in both cultivars.
- Taxon
- strawberry
_single2.jpg?width=60)
Fragaria ×ananassat0 - Organ / stage
- shoot tipse0
- Treatment
- MS + 0.3M sucrose for 40 h at 25℃e0
- Outcome
- regrowth ratee0
- Direction
- higheste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_single2.jpg?width=500)
Verbatim
e0“The highest regrowth rate (%) in both the cultivars was obtained when the shoot tips were precultured with MS + 0.3M sucrose for 40 h at 25℃”
t0“Strawberry”
Claim · model-written summary
Cryopreserved shoot tips had 55% regrowth with the stated culture sequence.
- Taxon
- strawberryFragaria ×ananassat0
- Treatment
- NH4NO3-free MS medium supplemented with 3% sucrose, 1.0 g/L casein, 1.0㎎/L GA3, and 0.5 ㎎/L BA for 5 weeks and in MS medium supplemented with 0.5 ㎎/L GA3 for 8 weekse0
- Outcome
- regrowth ratee0
- Value
- 55%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The cryopreserved shoots tips exhibited 55% regrowth rate by culturing in NH4NO3-free MS medium supplemented with 3% sucrose, 1.0 g/L casein, 1.0㎎/L GA3, and 0.5 ㎎/L BA for 5 weeks and in MS medium supplemented with 0.5 ㎎/L GA3 for 8 weeks”
t0“Strawberry”
Claim · model-written summary
The authors considered droplet-vitrification promising for strawberry germplasm cryostorage.
- Taxon
- strawberryFragaria ×ananassae0
- Outcome
- droplet-vitrification could be employed as a promising method for cryostorage of strawberry germplasme0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“droplet-vitrification could be employed as a promising method for cryostorage of strawberry germplasm”
The same questions in other papers
sucrose · growth · Fragaria x ananassa (strawberry)Positive and negative results
9 papers in the same direction as this paper · 2 in the opposite direction
- no effect
- negative
- positive
8 papers more in this group
See all 12 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.