Abstract
Abstract Melastoma malabathricum L. is a plant potentially useful in phytoremediation, and its successful propagation would facilitate further research in this application. The present study examined in vitro culture of leaf explants using MS medium supplemented with thidiazuron (TDZ; 0, 0.1, 1 and 2 mg/L) and 1-naphthaleneacetic acid (NAA; 0, 0.1 and 1 mg/L) alone and in combination. Leaf explants responded to all treatments by forming callus, except on medium with a combination of 2 mg mg/L TDZ and 1 mg/L NAA. The callus formed tended to be friable-compact and compact in texture, and browning tended to occur in callus formed on media containing NAA alone. The medium containing 1 mg mg/L NAA and 0.1 mg/L TDZ gave the best results for callus formation (100 %). Callus formed faster on medium containing 1 mg/L NAA alone. Both callus and adventitious roots formed on media containing NAA alone, with 1 mg/L NAA giving the best results. One explant growing on the medium containing 2 mg/L TDZ and 0.1 mg/L NAA formed both callus and shoots.
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Claims 7
Claim · model-written summary
Leaf explants formed callus under all treatments except 2 mg mg/L TDZ plus 1 mg/L NAA.
- Taxon
- Melastoma malabathricum L.
Melastoma malabathricumt0 - Organ / stage
- Leaf explantse0
- Outcome
- forming calluse0
- Qualifier
- except on medium with a combination of 2 mg mg/L TDZ and 1 mg/L NAAe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Leaf explants responded to all treatments by forming callus, except on medium with a combination of 2 mg mg/L TDZ and 1 mg/L NAA.”
t0“Melastoma malabathricum L.”
Claim · model-written summary
Callus texture tended to be friable-compact or compact.
- Taxon
- Melastoma malabathricum L.Melastoma malabathricumt0
- Outcome
- friable-compact and compact in texturee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The callus formed tended to be friable-compact and compact in texture”
t0“Melastoma malabathricum L.”
Claim · model-written summary
Callus on NAA-only media tended to brown.
- Taxon
- Melastoma malabathricum L.Melastoma malabathricumt0
- Treatment
- NAA alonee0
- Outcome
- browninge0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“browning tended to occur in callus formed on media containing NAA alone”
t0“Melastoma malabathricum L.”
Claim · model-written summary
Medium with 1 mg mg/L NAA and 0.1 mg/L TDZ yielded 100% callus formation.
- Taxon
- Melastoma malabathricum L.Melastoma malabathricumt0
- Treatment
- 1 mg mg/L NAA and 0.1 mg/L TDZe0
- Outcome
- callus formatione0
- Direction
- beste0
- Value
- 100 %e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The medium containing 1 mg mg/L NAA and 0.1 mg/L TDZ gave the best results for callus formation (100 %).”
t0“Melastoma malabathricum L.”
Claim · model-written summary
Callus formed faster with 1 mg/L NAA alone.
- Taxon
- Melastoma malabathricum L.Melastoma malabathricumt0
- Treatment
- 1 mg/L NAA alonee0
- Outcome
- Callus formede0
- Direction
- fastere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Callus formed faster on medium containing 1 mg/L NAA alone.”
t0“Melastoma malabathricum L.”
Claim · model-written summary
NAA-only media produced both callus and adventitious roots, with 1 mg/L giving the best result.
- Taxon
- Melastoma malabathricum L.Melastoma malabathricumt0
- Treatment
- NAA alonee0
- Outcome
- Both callus and adventitious roots formede0
- Qualifier
- 1 mg/L NAA giving the best resultse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Both callus and adventitious roots formed on media containing NAA alone, with 1 mg/L NAA giving the best results.”
t0“Melastoma malabathricum L.”
Claim · model-written summary
One explant on 2 mg/L TDZ plus 0.1 mg/L NAA formed callus and shoots.
- Taxon
- Melastoma malabathricum L.Melastoma malabathricumt0
- Treatment
- 2 mg/L TDZ and 0.1 mg/L NAAe0
- Outcome
- formed both callus and shootse0
- Value
- One explante0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“One explant growing on the medium containing 2 mg/L TDZ and 0.1 mg/L NAA formed both callus and shoots.”
t0“Melastoma malabathricum L.”