Abstract
BACKGROUND: The development of cryopreservation protocols for zygotic embryos has the advantage of direct embryo regeneration into plantlets, an important factor when one´s purpose is to preserve genetic plant resources. OBJECTIVE: Current study developed an efficient and simplified cryopreservation protocol for zygotic embryos of the macaw palm, a type of palm tree with great capacity for biodiesel production. MATERIALS AND METHODS: Embryos were obtained from seeds and rehydrated for 15 h in a 3% sucrose medium. Zygotic embryos were subsequently desiccated in a laminar flow cabinet for 0, 2, 4, 6 and 8 h. After each period, some embryos were cultured on a germination medium and others were placed in sterile cryotubes and immersed directly in liquid nitrogen for up to 360 d. Cryotubes were rewarmed by immersion at 40 degree C during 90 s (quick thawing). RESULTS: Germinability reduction occurred in proportion to desiccation time, with averages of 97 and 75% for 0 h and 8 h, respectively. In the case of cryopreserved zygotic embryos, the highest germination (81%) was obtained after 6-h desiccation, when the embryos had approximately 10% water content (FW basis). CONCLUSION: The zygotic embryos cryopreservation protocol covered in this study represents another option for preserving Acrocomia aculeata genetic resources.
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Claims 3
Claim · model-written summary
Germinability of macaw palm zygotic embryos fell with desiccation time, from 97% at 0 h to 75% at 8 h.
- Taxon
- Acrocomia aculeata
Acrocomia aculeatat0 - Treatment
- desiccation timee1
- Outcome
- germinabilitye1
- Direction
- reductione1
- Value
- 97 and 75%e1
- Comparator
- 0 he1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Germinability reduction occurred in proportion to desiccation time, with averages of 97 and 75% for 0 h and 8 h”
t0“Acrocomia aculeata (Jacq.) Lodd. Ex Mart.”
Claim · model-written summary
Cryopreserved zygotic embryos reached the highest germination (81%) after 6-h desiccation, at about 10% water content.
- Taxon
- Acrocomia aculeataAcrocomia aculeatat0
- Organ / stage
- zygotic embryose1
- Treatment
- 6-h desiccatione1
- Outcome
- germinatione1
- Direction
- higheste1
- Value
- 81%e1
- Qualifier
- approximately 10% water content (FW basis)e1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“In the case of cryopreserved zygotic embryos, the highest germination (81%) was obtained after 6-h desiccation, when the embryos had approximately 10% water content (FW basis)”
t0“Acrocomia aculeata (Jacq.) Lodd. Ex Mart.”
Claim · model-written summary
The zygotic embryo cryopreservation protocol is another option for preserving Acrocomia aculeata genetic resources.
- Taxon
- Acrocomia aculeataAcrocomia aculeatae1
- Outcome
- preserving Acrocomia aculeata genetic resourcese1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“The zygotic embryos cryopreservation protocol covered in this study represents another option for preserving Acrocomia aculeata genetic resources”