Paper · 1973 · ScholarsArchive (Brigham Young University)

The effects of deficiencies of certain mineral nutrients on the activity and various properties of ribulose-1,5-diphosphate carboxylase from barley (Hordeum vulgare L.)

Philip Alan Harrison

13
claims
26
verbatim quotes
0
citations
1
research areas
✓
Open access

Abstract

Barley plants grown in Mg, Mn, Zn and Cu deficient media were analyzed for specific activity, total activity and amount of RuDPCase present. Percent dry weight, as well as chlorophyll and soluble protein content were determined. All deficiencies caused lower chlorophyll and RuDPCase concentrations than occurred in the control. Soluble protein content, however, was higher in all deficient plants than in the control, either on a dry or a fresh weight basis. The addition of 18 μM Mn^2+ increased the activity of Mg^2+ containing RuDPCase preparations as much as 133% above normal. The specific activity of RuDPCase from Mg, Mn, Zn and Cu deficient plants was 60%, 118%, 159% and 149%, respectively, of the control. After 20 hours preincubation with Mg^2+, RuDPCase from Mg deficient plants attained 96% of the specific activity exhibited by the control. Total activity of RuDPCase from Mg, Mn, Zn and Cu deficient plants was 22%, 51%, 102% and 12 1%, respectively, of the control. Possible roles of Mn^2+ and Cu^2+ in the activation of RuDPCase are discussed.

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Claims 13

Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

All tested mineral deficiencies lowered barley chlorophyll concentration.

Treatment
Mg, Mn, Zn and Cu deficient mediae1
Outcome
chlorophylle0
Direction
lowere0
Comparator
controle0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Hordeum vulgare
Hordeum vulgare · TaxonJackyM59 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“All deficiencies caused lower chlorophyll and RuDPCase concentrations than occurred in the control.”

✓ located verbatim in the source · abstract, characters 229–328 · show in text ↑
e1

“Barley plants grown in Mg, Mn, Zn and Cu deficient media”

✓ located verbatim in the source · abstract, characters 0–56 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

All tested mineral deficiencies lowered barley RuDPCase concentration.

Treatment
Mg, Mn, Zn and Cu deficient mediae1
Gene
RuDPCasee0
Outcome
RuDPCase concentrationse0
Direction
lowere0
Comparator
controle0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Hordeum vulgare
Hordeum vulgare · TaxonJackyM59 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“All deficiencies caused lower chlorophyll and RuDPCase concentrations than occurred in the control.”

✓ located verbatim in the source · abstract, characters 229–328 · show in text ↑
e1

“Barley plants grown in Mg, Mn, Zn and Cu deficient media”

✓ located verbatim in the source · abstract, characters 0–56 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

All deficient barley plants had higher soluble protein than controls.

Treatment
Mg, Mn, Zn and Cu deficient mediae1
Outcome
Soluble protein contente0
Direction
highere0
Comparator
controle0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Hordeum vulgare
Hordeum vulgare · TaxonJackyM59 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Soluble protein content, however, was higher in all deficient plants than in the control, either on a dry or a fresh weight basis.”

✓ located verbatim in the source · abstract, characters 329–459 · show in text ↑
e1

“Barley plants grown in Mg, Mn, Zn and Cu deficient media”

✓ located verbatim in the source · abstract, characters 0–56 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Adding 18 μM Mn²⁺ increased RuDPCase activity as much as 133% above normal.

Treatment
addition of 18 μM Mn^2+e0
Gene
RuDPCasee0
Outcome
activity of Mg^2+ containing RuDPCase preparationse0
Direction
increasede0
Value
133%e0
Comparator
normale0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Hordeum vulgare
Hordeum vulgare · TaxonJackyM59 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“The addition of 18 μM Mn^2+ increased the activity of Mg^2+ containing RuDPCase preparations as much as 133% above normal.”

✓ located verbatim in the source · abstract, characters 460–582 · show in text ↑
e1

“Barley plants”

✓ located verbatim in the source · abstract, characters 0–13 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

RuDPCase specific activity in Mg-deficient barley was 60% of control.

Treatment
Mge0
Gene
RuDPCasee0
Outcome
specific activity of RuDPCasee0
Value
60%e0
Comparator
controle0

Not reportedDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Hordeum vulgare
Hordeum vulgare · TaxonJackyM59 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“The specific activity of RuDPCase from Mg, Mn, Zn and Cu deficient plants was 60%, 118%, 159% and 149%, respectively, of the control.”

✓ located verbatim in the source · abstract, characters 583–716 · show in text ↑
e1

“Barley plants”

✓ located verbatim in the source · abstract, characters 0–13 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

RuDPCase specific activity in Mn-deficient barley was 118% of control.

Treatment
Mne0
Gene
RuDPCasee0
Outcome
specific activity of RuDPCasee0
Value
118%e0
Comparator
controle0

Not reportedDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Hordeum vulgare
Hordeum vulgare · TaxonJackyM59 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“The specific activity of RuDPCase from Mg, Mn, Zn and Cu deficient plants was 60%, 118%, 159% and 149%, respectively, of the control.”

✓ located verbatim in the source · abstract, characters 583–716 · show in text ↑
e1

“Barley plants”

✓ located verbatim in the source · abstract, characters 0–13 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

RuDPCase specific activity in Zn-deficient barley was 159% of control.

Treatment
Zne0
Gene
RuDPCasee0
Outcome
specific activity of RuDPCasee0
Value
159%e0
Comparator
controle0

Not reportedDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Hordeum vulgare
Hordeum vulgare · TaxonJackyM59 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“The specific activity of RuDPCase from Mg, Mn, Zn and Cu deficient plants was 60%, 118%, 159% and 149%, respectively, of the control.”

✓ located verbatim in the source · abstract, characters 583–716 · show in text ↑
e1

“Barley plants”

✓ located verbatim in the source · abstract, characters 0–13 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

RuDPCase specific activity in Cu-deficient barley was 149% of control.

Treatment
Cue0
Gene
RuDPCasee0
Outcome
specific activity of RuDPCasee0
Value
149%e0
Comparator
controle0

Not reportedDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Hordeum vulgare
Hordeum vulgare · TaxonJackyM59 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“The specific activity of RuDPCase from Mg, Mn, Zn and Cu deficient plants was 60%, 118%, 159% and 149%, respectively, of the control.”

✓ located verbatim in the source · abstract, characters 583–716 · show in text ↑
e1

“Barley plants”

✓ located verbatim in the source · abstract, characters 0–13 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

After Mg²⁺ preincubation, RuDPCase from Mg-deficient barley reached 96% of control specific activity.

Treatment
20 hours preincubation with Mg^2+e0
Gene
RuDPCasee0
Outcome
specific activitye0
Value
96%e0
Comparator
controle0

Not reportedDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Hordeum vulgare
Hordeum vulgare · TaxonJackyM59 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“After 20 hours preincubation with Mg^2+, RuDPCase from Mg deficient plants attained 96% of the specific activity exhibited by the control.”

✓ located verbatim in the source · abstract, characters 717–855 · show in text ↑
e1

“Barley plants”

✓ located verbatim in the source · abstract, characters 0–13 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Total RuDPCase activity in Mg-deficient barley was 22% of control.

Treatment
Mge0
Gene
RuDPCasee0
Outcome
Total activity of RuDPCasee0
Value
22%e0
Comparator
controle0

Not reportedDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Hordeum vulgare
Hordeum vulgare · TaxonJackyM59 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Total activity of RuDPCase from Mg, Mn, Zn and Cu deficient plants was 22%, 51%, 102% and 12 1%, respectively, of the control.”

✓ located verbatim in the source · abstract, characters 856–982 · show in text ↑
e1

“Barley plants”

✓ located verbatim in the source · abstract, characters 0–13 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Total RuDPCase activity in Mn-deficient barley was 51% of control.

Treatment
Mne0
Gene
RuDPCasee0
Outcome
Total activity of RuDPCasee0
Value
51%e0
Comparator
controle0

Not reportedDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Hordeum vulgare
Hordeum vulgare · TaxonJackyM59 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Total activity of RuDPCase from Mg, Mn, Zn and Cu deficient plants was 22%, 51%, 102% and 12 1%, respectively, of the control.”

✓ located verbatim in the source · abstract, characters 856–982 · show in text ↑
e1

“Barley plants”

✓ located verbatim in the source · abstract, characters 0–13 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Total RuDPCase activity in Zn-deficient barley was 102% of control.

Treatment
Zne0
Gene
RuDPCasee0
Outcome
Total activity of RuDPCasee0
Value
102%e0
Comparator
controle0

Not reportedDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Hordeum vulgare
Hordeum vulgare · TaxonJackyM59 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Total activity of RuDPCase from Mg, Mn, Zn and Cu deficient plants was 22%, 51%, 102% and 12 1%, respectively, of the control.”

✓ located verbatim in the source · abstract, characters 856–982 · show in text ↑
e1

“Barley plants”

✓ located verbatim in the source · abstract, characters 0–13 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Total RuDPCase activity in Cu-deficient barley was reported as 12 1% of control.

Treatment
Cue0
Gene
RuDPCasee0
Outcome
Total activity of RuDPCasee0
Value
12 1%e0
Comparator
controle0

Not reportedDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Hordeum vulgare
Hordeum vulgare · TaxonJackyM59 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Total activity of RuDPCase from Mg, Mn, Zn and Cu deficient plants was 22%, 51%, 102% and 12 1%, respectively, of the control.”

✓ located verbatim in the source · abstract, characters 856–982 · show in text ↑
e1

“Barley plants”

✓ located verbatim in the source · abstract, characters 0–13 · show in text ↑

zinc atom · physiology: pigments · Hordeum vulgare (barley)Positive and negative results

3 papers: 1 positive, 2 negative or no effect

1 paper in the same direction as this paper · 1 in the opposite direction

  1. positive
  2. negative
See all 3 papers →

manganese atom · physiology: pigments · Hordeum vulgare (barley)Positive and negative results

4 papers: 2 positive, 2 negative or no effect

1 paper in the same direction as this paper · 2 in the opposite direction

  1. positive
  2. positive
  3. negative
See all 4 papers →

copper atom · physiology: pigments · Hordeum vulgare (barley)Positive and negative results

5 papers: 2 positive, 3 negative or no effect

1 paper in the same direction as this paper · 3 in the opposite direction

This paper is about lacking the nutrient, so it counts the other way round here.

  1. negative
  2. negative
  3. negative

1 paper more in this group

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magnesium atom · physiology: pigments · Hordeum vulgare (barley)Positive and negative results

5 papers: 3 positive, 2 negative or no effect

1 paper in the same direction as this paper · 3 in the opposite direction

  1. positive
  2. positive
  3. positive

1 paper more in this group

See all 5 papers →

manganese atom · nutrient: content · Hordeum vulgare (barley)Positive and negative results

16 papers: 11 positive, 5 negative or no effect

10 papers in the same direction as this paper · 4 in the opposite direction · 1 reporting no difference from an alternative

  1. negative
  2. no effect
  3. no effect

12 papers more in this group

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in vitro · physiology: pigments · Hordeum vulgare (barley)Positive and negative results

3 papers: 1 positive, 2 negative or no effect

1 paper in the same direction as this paper · 1 in the opposite direction

  1. positive
  2. negative
See all 3 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.