Abstract
This study was aimed to induce and produce a polyploidy Robinia pseudoacacia seedling by different concentrations of colchicine (0.0%, 0.5%, 1.0%, 1.5%, and 2.0%) in various exposure times (12, 24 and 48 h), using in two experiments. In the first, were applied to the seeds by immersing method. In the second, were applied to shoot apical meristems by dropping method. The results showed significant effects of different interactions between colchicine concentration and exposure period on the seed germination, seedling survival rate and seedling characters. By increasing of the colchicine concentration the seed germination and seedling survival rate decreased significantly in colchicine concentrations (0.15 and 0.2%). Frequency of stomata decreased and on the opposite the stomata length and width increased with increases the colchicine concentrations. Polyploidy seedlings show vegetative growth superiority yield compared to diploids seedlings, leaf area in treated plants was larger and with deep green pigmentation as compared to the control seedlings. Seed treatment method was found to be more efficient than shoot apical meristems treatment method in producing tetraploid and mixoploid plants, colchicine solutions at 0.1% and 1.5% in 24 h. and 48 h. was found most effectively produced polyploidy and inducing variation in both investigations.
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Claims 7
Claim · model-written summary
seed germination and seedling survival rate was decreased.
- Taxon
- Robinia pseudoacacia L.
Robinia pseudoacaciat0 - Treatment
- colchicine concentrationse0
- Outcome
- seed germination and seedling survival ratee0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Robinia pseudoacacia L.”
e0“By increasing of the colchicine concentration the seed germination and seedling survival rate decreased significantly in colchicine concentrations (0.15 and 0.2%).”
Claim · model-written summary
Frequency of stomata was decreased.
- Taxon
- Robinia pseudoacacia L.Robinia pseudoacaciat0
- Treatment
- colchicine concentrationse0
- Outcome
- Frequency of stomatae0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Robinia pseudoacacia L.”
e0“Frequency of stomata decreased and on the opposite the stomata length and width increased with increases the colchicine concentrations.”
Claim · model-written summary
stomata length and width was increased.
- Taxon
- Robinia pseudoacacia L.Robinia pseudoacaciat0
- Treatment
- colchicine concentrationse0
- Outcome
- stomata length and widthe0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Robinia pseudoacacia L.”
e0“Frequency of stomata decreased and on the opposite the stomata length and width increased with increases the colchicine concentrations.”
Claim · model-written summary
vegetative growth superiority yield was superiority.
- Taxon
- Robinia pseudoacacia L.Robinia pseudoacaciat0
- Treatment
- Polyploidy seedlingse0
- Outcome
- vegetative growth superiority yielde0
- Direction
- superioritye0
- Comparator
- diploids seedlingse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Robinia pseudoacacia L.”
e0“Polyploidy seedlings show vegetative growth superiority yield compared to diploids seedlings, leaf area in treated plants was larger and with deep green pigmentation as compared to the control seedlings.”
Claim · model-written summary
leaf area was larger.
- Taxon
- Robinia pseudoacacia L.Robinia pseudoacaciat0
- Treatment
- Polyploidy seedlingse0
- Outcome
- leaf areae0
- Direction
- largere0
- Comparator
- control seedlingse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Robinia pseudoacacia L.”
e0“Polyploidy seedlings show vegetative growth superiority yield compared to diploids seedlings, leaf area in treated plants was larger and with deep green pigmentation as compared to the control seedlings.”
Claim · model-written summary
Seed treatment was more efficient than shoot-apex treatment in producing tetraploid and mixoploid plants.
- Taxon
- Robinia pseudoacacia L.Robinia pseudoacaciat0
- Treatment
- Seed treatment methode0
- Outcome
- producing tetraploid and mixoploid plantse0
- Direction
- more efficiente0
- Comparator
- shoot apical meristems treatment methode0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Robinia pseudoacacia L.”
e0“Seed treatment method was found to be more efficient than shoot apical meristems treatment method in producing tetraploid and mixoploid plants, colchicine solutions at 0.1% and 1.5% in 24 h. and 48 h. was found most effectively produced polyploidy and inducing variation in both investigations.”
Claim · model-written summary
The specified colchicine concentrations and durations most effectively induced polyploidy and variation.
- Taxon
- Robinia pseudoacacia L.Robinia pseudoacaciat0
- Treatment
- colchicine solutions at 0.1% and 1.5% in 24 h. and 48 h.e0
- Outcome
- polyploidy and inducing variatione0
- Direction
- most effectivelye0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Robinia pseudoacacia L.”
e0“Seed treatment method was found to be more efficient than shoot apical meristems treatment method in producing tetraploid and mixoploid plants, colchicine solutions at 0.1% and 1.5% in 24 h. and 48 h. was found most effectively produced polyploidy and inducing variation in both investigations.”
The same questions in other papers
colchicine · growth · Robinia pseudoacacia (black locust)Positive and negative results
1 in the opposite direction
- positive
colchicine · physiology: gas exchange · Robinia pseudoacacia (black locust)Results agree
1 paper in the same direction as this paper
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.