Abstract
Effects of different nutrient media, vitamins and peptone on in vitro asymbiotic seed germination of Geodorum densiflorum (Lam.) Schltr. were studied. In vitro developed rhizomes were used to determine the influence of cytokinins on growth and morphogenesis. Seed germination and survival rate of protocorms were highest in MS medium compared to other basal media. The protocorms raised through seed germination directly proliferated into rhizomes in later phase of growth and development. Nodal portions of rhizomes further exhibited growth through formation of direct and callus mediated protocorm like bodies. In general, three types of rhizome movements were noticed; viz. positively geotropic, negatively geotropic and diagravitropic movements. In the PGR free control and in the presence of NAA alone rhizomes exhibited positively geotropic movements. On the contrary, presence of cytokinin either alone or in combination with NAA exhibited diagravitropic movements. Application of TDZ completely suppressed the positively geotropic movement and enhanced the frequency of negatively geotropic movement of rhizomes followed by shoot bud formation. BAP was most effective cytokinin for large scale rhizome mediated multiplication of Geodorum densiflorum and TDZ could be effectively employed for rapid leafy shoot regeneration. Plant Tissue Cult. & Biotech. 30(1): 65-75, 2020 (June)
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Claims 10
Claim · model-written summary
MS medium gave the highest seed germination and protocorm survival.
- Taxon
- Geodorum densiflorum
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Eulophia pictat0 - Treatment
- MS mediume0
- Outcome
- Seed germination and survival rate of protocormse0
- Direction
- higheste0
- Comparator
- other basal mediae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“Seed germination and survival rate of protocorms were highest in MS medium compared to other basal media.”
t0“Geodorum densiflorum”
Claim · model-written summary
Protocorms directly formed rhizomes.
- Taxon
- Geodorum densiflorumEulophia pictat0
- Outcome
- directly proliferated into rhizomese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The protocorms raised through seed germination directly proliferated into rhizomes in later phase of growth and development.”
t0“Geodorum densiflorum”
Claim · model-written summary
Rhizome nodes formed direct and callus mediated protocorm like bodies.
- Taxon
- Geodorum densiflorumEulophia pictat0
- Outcome
- formation of direct and callus mediated protocorm like bodiese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Nodal portions of rhizomes further exhibited growth through formation of direct and callus mediated protocorm like bodies.”
t0“Geodorum densiflorum”
Claim · model-written summary
Rhizomes showed three geotropic movement types.
- Taxon
- Geodorum densiflorumEulophia pictat0
- Outcome
- positively geotropic, negatively geotropic and diagravitropic movementse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“three types of rhizome movements were noticed; viz. positively geotropic, negatively geotropic and diagravitropic movements.”
t0“Geodorum densiflorum”
Claim · model-written summary
Control and NAA alone yielded positive geotropic movement.
- Taxon
- Geodorum densiflorumEulophia pictat0
- Outcome
- positively geotropic movementse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In the PGR free control and in the presence of NAA alone rhizomes exhibited positively geotropic movements.”
t0“Geodorum densiflorum”
Claim · model-written summary
Cytokinin alone or with NAA produced diagravitropic rhizome movement.
- Taxon
- Geodorum densiflorumEulophia pictat0
- Treatment
- cytokinin either alone or in combination with NAAe0
- Outcome
- diagravitropic movementse0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“presence of cytokinin either alone or in combination with NAA exhibited diagravitropic movements.”
t0“Geodorum densiflorum”
Claim · model-written summary
TDZ suppressed positive geotropic movement.
- Taxon
- Geodorum densiflorumEulophia pictat0
- Treatment
- TDZe0
- Outcome
- positively geotropic movemente0
- Direction
- suppressede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Application of TDZ completely suppressed the positively geotropic movement”
t0“Geodorum densiflorum”
Claim · model-written summary
TDZ enhanced negative geotropism and was followed by shoot bud formation.
- Taxon
- Geodorum densiflorumEulophia pictat0
- Treatment
- TDZe0
- Outcome
- frequency of negatively geotropic movement of rhizomese0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Application of TDZ completely suppressed the positively geotropic movement and enhanced the frequency of negatively geotropic movement of rhizomes followed by shoot bud formation.”
t0“Geodorum densiflorum”
Claim · model-written summary
BAP was most effective for rhizome multiplication.
- Taxon
- Geodorum densiflorumEulophia pictae0
- Treatment
- BAPe0
- Outcome
- large scale rhizome mediated multiplicatione0
- Direction
- most effectivee0
- Comparator
- cytokinine0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“BAP was most effective cytokinin for large scale rhizome mediated multiplication of Geodorum densiflorum”
Claim · model-written summary
TDZ was proposed for rapid leafy shoot regeneration.
- Taxon
- Geodorum densiflorumEulophia pictat0
- Outcome
- rapid leafy shoot regeneratione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“TDZ could be effectively employed for rapid leafy shoot regeneration.”
t0“Geodorum densiflorum”
The same questions in other papers
in vitro · growth · Eulophia pictaResults agree
3 papers in the same direction as this paper
- positive
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.