Abstract
Seeds of Geodorum densiflorum (Lam.) Schltr. were aseptically cultured on 0.8% (w/v) agar solidified Murashige and Skoog (MS) and Phytamax (PM) media. Seeds germinated and formed light green globular structures on both the media. These globular structures failed to produce seedlings on PM medium, where it proliferated and developed into irregular-shaped rhizomes with white hairy structures. On the other hand, on MS medium the globular structures directly produced tiny seedlings which underwent elongation in PGRs supplemented MS medium. A composition of 2.0 mg/l NAA and 2.0 mg/l BAP proved best for enhancing elongation. The elongated seedlings were grown on half and full strength MS medium with or without IAA and activated charcoal (AC). For sound development of the root system MS + 3% (w/v) sucrose + 1.0 mg/l IAA + 0.1% (w/v) AC proved best. The irregular shaped rhizomes as developed on PM medium were further used for mass production of seedlings. Rhizome tips produced multiple shoot buds on PGRs (NAA, IAA, IBA, picloram, BAP and zeatin) supplemented media. The highest number of multiple shoot buds per segment was observed on 0.8% (w/v) agar solidified MS + 3% (w/v) sucrose + 2.0 mg/l BAP. The multiple shoot buds underwent elongation on the same media and the maximum rate of elongation was recorded on 0.8% (w/v) agar solidified MS + 3% (w/v) sucrose + 2.0 mg/l NAA + 2.0 mg/l BAP. The elongated seedlings were rooted in the rooting medium and the plantlets after acclimatization were established in pot.
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Claims 9
Claim · model-written summary
Seeds germinated and made green globular structures on both media.
- Taxon
- Geodorum densiflorum (Lam.) Schltr.
Eulophia pictat0 - Treatment
- both the mediae0
- Outcome
- germinated and formed light green globular structurese0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
t0“Geodorum densiflorum (Lam.) Schltr.”
e0“Seeds germinated and formed light green globular structures on both the media.”
Claim · model-written summary
On PM medium the globular structures formed rhizomes instead of seedlings.
- Taxon
- Geodorum densiflorum (Lam.) Schltr.
Eulophia pictat0 - Organ / stage
- globular structurese0
- Treatment
- PM mediume0
- Outcome
- failed to produce seedlingse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
t0“Geodorum densiflorum (Lam.) Schltr.”
e0“These globular structures failed to produce seedlings on PM medium, where it proliferated and developed into irregular-shaped rhizomes with white hairy structures.”
Claim · model-written summary
On MS medium globular structures directly made seedlings that elongated with PGR supplementation.
- Taxon
- Geodorum densiflorum (Lam.) Schltr.
Eulophia pictat0 - Treatment
- PGRs supplemented MS mediume0
- Outcome
- directly produced tiny seedlings which underwent elongatione0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
t0“Geodorum densiflorum (Lam.) Schltr.”
e0“on MS medium the globular structures directly produced tiny seedlings which underwent elongation in PGRs supplemented MS medium.”
Claim · model-written summary
NAA and BAP at 2.0 mg/l each best enhanced elongation.
- Taxon
- Geodorum densiflorum (Lam.) Schltr.
Eulophia pictat0 - Treatment
- 2.0 mg/l NAA and 2.0 mg/l BAPe0
- Outcome
- elongatione0
- Direction
- beste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
t0“Geodorum densiflorum (Lam.) Schltr.”
e0“A composition of 2.0 mg/l NAA and 2.0 mg/l BAP proved best for enhancing elongation.”
Claim · model-written summary
MS medium with sucrose, IAA and activated charcoal was best for root development.
- Taxon
- Geodorum densiflorum (Lam.) Schltr.
Eulophia pictat0 - Treatment
- MS + 3% (w/v) sucrose + 1.0 mg/l IAA + 0.1% (w/v) ACe0
- Outcome
- development of the root systeme0
- Direction
- beste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
t0“Geodorum densiflorum (Lam.) Schltr.”
e0“For sound development of the root system MS + 3% (w/v) sucrose + 1.0 mg/l IAA + 0.1% (w/v) AC proved best.”
Claim · model-written summary
Rhizome tips formed multiple shoot buds on regulator supplemented media.
- Taxon
- Geodorum densiflorum (Lam.) Schltr.
Eulophia pictat0 - Organ / stage
- Rhizome tipse0
- Treatment
- PGRs (NAA, IAA, IBA, picloram, BAP and zeatin) supplemented mediae0
- Outcome
- multiple shoot budse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
t0“Geodorum densiflorum (Lam.) Schltr.”
e0“Rhizome tips produced multiple shoot buds on PGRs (NAA, IAA, IBA, picloram, BAP and zeatin) supplemented media.”
Claim · model-written summary
MS with sucrose and BAP gave the most shoot buds per segment.
- Taxon
- Geodorum densiflorum (Lam.) Schltr.
Eulophia pictat0 - Treatment
- 0.8% (w/v) agar solidified MS + 3% (w/v) sucrose + 2.0 mg/l BAPe0
- Outcome
- number of multiple shoot buds per segmente0
- Direction
- higheste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
t0“Geodorum densiflorum (Lam.) Schltr.”
e0“The highest number of multiple shoot buds per segment was observed on 0.8% (w/v) agar solidified MS + 3% (w/v) sucrose + 2.0 mg/l BAP.”
Claim · model-written summary
MS with sucrose, NAA and BAP gave the greatest shoot elongation rate.
- Taxon
- Geodorum densiflorum (Lam.) Schltr.
Eulophia pictat0 - Treatment
- 0.8% (w/v) agar solidified MS + 3% (w/v) sucrose + 2.0 mg/l NAA + 2.0 mg/l BAPe0
- Outcome
- rate of elongatione0
- Direction
- maximume0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
t0“Geodorum densiflorum (Lam.) Schltr.”
e0“the maximum rate of elongation was recorded on 0.8% (w/v) agar solidified MS + 3% (w/v) sucrose + 2.0 mg/l NAA + 2.0 mg/l BAP.”
Claim · model-written summary
Rooted seedlings survived acclimatization and establishment in pots.
- Taxon
- Geodorum densiflorum (Lam.) Schltr.
Eulophia pictat0 - Outcome
- plantlets after acclimatization were established in pote0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
t0“Geodorum densiflorum (Lam.) Schltr.”
e0“The elongated seedlings were rooted in the rooting medium and the plantlets after acclimatization were established in pot.”
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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.