Paper · 2003

In vitro Germination and Micropropagation of Geodorum densiflorum (Lam.) Schltr., an Endangered

S. K. Bhadra; MM Hossain

9
claims
18
verbatim quotes
1
citations
2
research areas
—
Not open access

Abstract

Seeds of Geodorum densiflorum (Lam.) Schltr. were aseptically cultured on 0.8% (w/v) agar solidified Murashige and Skoog (MS) and Phytamax (PM) media. Seeds germinated and formed light green globular structures on both the media. These globular structures failed to produce seedlings on PM medium, where it proliferated and developed into irregular-shaped rhizomes with white hairy structures. On the other hand, on MS medium the globular structures directly produced tiny seedlings which underwent elongation in PGRs supplemented MS medium. A composition of 2.0 mg/l NAA and 2.0 mg/l BAP proved best for enhancing elongation. The elongated seedlings were grown on half and full strength MS medium with or without IAA and activated charcoal (AC). For sound development of the root system MS + 3% (w/v) sucrose + 1.0 mg/l IAA + 0.1% (w/v) AC proved best. The irregular shaped rhizomes as developed on PM medium were further used for mass production of seedlings. Rhizome tips produced multiple shoot buds on PGRs (NAA, IAA, IBA, picloram, BAP and zeatin) supplemented media. The highest number of multiple shoot buds per segment was observed on 0.8% (w/v) agar solidified MS + 3% (w/v) sucrose + 2.0 mg/l BAP. The multiple shoot buds underwent elongation on the same media and the maximum rate of elongation was recorded on 0.8% (w/v) agar solidified MS + 3% (w/v) sucrose + 2.0 mg/l NAA + 2.0 mg/l BAP. The elongated seedlings were rooted in the rooting medium and the plantlets after acclimatization were established in pot.

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Claims 9

Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Seeds germinated and made green globular structures on both media.

Taxon
Geodorum densiflorum (Lam.) Schltr.Eulophia pictat0
Treatment
both the mediae0
Outcome
germinated and formed light green globular structurese0

Not reportedComparatorDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Eulophia picta
Eulophia picta · Taxon · picture from its Wikimedia Commons categoryNativeplants garden · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“Geodorum densiflorum (Lam.) Schltr.”

✓ located verbatim in the source · title, characters 45–80 · show in text ↑
e0

“Seeds germinated and formed light green globular structures on both the media.”

✓ located verbatim in the source · abstract, characters 151–229 · show in text ↑
Observationobservedoriginal resultnegativemeasured

Claim · model-written summary

On PM medium the globular structures formed rhizomes instead of seedlings.

Taxon
Geodorum densiflorum (Lam.) Schltr.Eulophia pictat0
Organ / stage
globular structurese0
Treatment
PM mediume0
Outcome
failed to produce seedlingse0

Not reportedDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Eulophia picta
Eulophia picta · Taxon · picture from its Wikimedia Commons categoryNativeplants garden · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“Geodorum densiflorum (Lam.) Schltr.”

✓ located verbatim in the source · title, characters 45–80 · show in text ↑
e0

“These globular structures failed to produce seedlings on PM medium, where it proliferated and developed into irregular-shaped rhizomes with white hairy structures.”

✓ located verbatim in the source · abstract, characters 230–393 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

On MS medium globular structures directly made seedlings that elongated with PGR supplementation.

Taxon
Geodorum densiflorum (Lam.) Schltr.Eulophia pictat0
Treatment
PGRs supplemented MS mediume0
Outcome
directly produced tiny seedlings which underwent elongatione0

Not reportedDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Eulophia picta
Eulophia picta · Taxon · picture from its Wikimedia Commons categoryNativeplants garden · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“Geodorum densiflorum (Lam.) Schltr.”

✓ located verbatim in the source · title, characters 45–80 · show in text ↑
e0

“on MS medium the globular structures directly produced tiny seedlings which underwent elongation in PGRs supplemented MS medium.”

✓ located verbatim in the source · abstract, characters 413–541 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

NAA and BAP at 2.0 mg/l each best enhanced elongation.

Taxon
Geodorum densiflorum (Lam.) Schltr.Eulophia pictat0
Treatment
2.0 mg/l NAA and 2.0 mg/l BAPe0
Outcome
elongatione0
Direction
beste0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Eulophia picta
Eulophia picta · Taxon · picture from its Wikimedia Commons categoryNativeplants garden · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“Geodorum densiflorum (Lam.) Schltr.”

✓ located verbatim in the source · title, characters 45–80 · show in text ↑
e0

“A composition of 2.0 mg/l NAA and 2.0 mg/l BAP proved best for enhancing elongation.”

✓ located verbatim in the source · abstract, characters 542–626 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

MS medium with sucrose, IAA and activated charcoal was best for root development.

Taxon
Geodorum densiflorum (Lam.) Schltr.Eulophia pictat0
Treatment
MS + 3% (w/v) sucrose + 1.0 mg/l IAA + 0.1% (w/v) ACe0
Outcome
development of the root systeme0
Direction
beste0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Eulophia picta
Eulophia picta · Taxon · picture from its Wikimedia Commons categoryNativeplants garden · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“Geodorum densiflorum (Lam.) Schltr.”

✓ located verbatim in the source · title, characters 45–80 · show in text ↑
e0

“For sound development of the root system MS + 3% (w/v) sucrose + 1.0 mg/l IAA + 0.1% (w/v) AC proved best.”

✓ located verbatim in the source · abstract, characters 747–853 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Rhizome tips formed multiple shoot buds on regulator supplemented media.

Taxon
Geodorum densiflorum (Lam.) Schltr.Eulophia pictat0
Organ / stage
Rhizome tipse0
Treatment
PGRs (NAA, IAA, IBA, picloram, BAP and zeatin) supplemented mediae0
Outcome
multiple shoot budse0

Not reportedDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Eulophia picta
Eulophia picta · Taxon · picture from its Wikimedia Commons categoryNativeplants garden · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“Geodorum densiflorum (Lam.) Schltr.”

✓ located verbatim in the source · title, characters 45–80 · show in text ↑
e0

“Rhizome tips produced multiple shoot buds on PGRs (NAA, IAA, IBA, picloram, BAP and zeatin) supplemented media.”

✓ located verbatim in the source · abstract, characters 962–1073 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

MS with sucrose and BAP gave the most shoot buds per segment.

Taxon
Geodorum densiflorum (Lam.) Schltr.Eulophia pictat0
Treatment
0.8% (w/v) agar solidified MS + 3% (w/v) sucrose + 2.0 mg/l BAPe0
Outcome
number of multiple shoot buds per segmente0
Direction
higheste0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Eulophia picta
Eulophia picta · Taxon · picture from its Wikimedia Commons categoryNativeplants garden · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“Geodorum densiflorum (Lam.) Schltr.”

✓ located verbatim in the source · title, characters 45–80 · show in text ↑
e0

“The highest number of multiple shoot buds per segment was observed on 0.8% (w/v) agar solidified MS + 3% (w/v) sucrose + 2.0 mg/l BAP.”

✓ located verbatim in the source · abstract, characters 1074–1208 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

MS with sucrose, NAA and BAP gave the greatest shoot elongation rate.

Taxon
Geodorum densiflorum (Lam.) Schltr.Eulophia pictat0
Treatment
0.8% (w/v) agar solidified MS + 3% (w/v) sucrose + 2.0 mg/l NAA + 2.0 mg/l BAPe0
Outcome
rate of elongatione0
Direction
maximume0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Eulophia picta
Eulophia picta · Taxon · picture from its Wikimedia Commons categoryNativeplants garden · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“Geodorum densiflorum (Lam.) Schltr.”

✓ located verbatim in the source · title, characters 45–80 · show in text ↑
e0

“the maximum rate of elongation was recorded on 0.8% (w/v) agar solidified MS + 3% (w/v) sucrose + 2.0 mg/l NAA + 2.0 mg/l BAP.”

✓ located verbatim in the source · abstract, characters 1276–1402 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Rooted seedlings survived acclimatization and establishment in pots.

Taxon
Geodorum densiflorum (Lam.) Schltr.Eulophia pictat0
Outcome
plantlets after acclimatization were established in pote0

Not reportedDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Eulophia picta
Eulophia picta · Taxon · picture from its Wikimedia Commons categoryNativeplants garden · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“Geodorum densiflorum (Lam.) Schltr.”

✓ located verbatim in the source · title, characters 45–80 · show in text ↑
e0

“The elongated seedlings were rooted in the rooting medium and the plantlets after acclimatization were established in pot.”

✓ located verbatim in the source · abstract, characters 1403–1525 · show in text ↑

N-benzyladenine · growth · Eulophia pictaPositive and negative results

3 papers: 2 positive, 1 negative or no effect

1 paper in the same direction as this paper · 1 in the opposite direction

  1. negative
  2. positive
See all 3 papers →

1-naphthaleneacetic acid · growth · Eulophia pictaResults agree

2 papers: 2 positive, 0 negative or no effect

1 paper in the same direction as this paper

  1. positive
See all 2 papers →

in vitro · growth · Eulophia pictaResults agree

4 papers: 4 positive, 0 negative or no effect

3 papers in the same direction as this paper

  1. positive
  2. positive
  3. positive
See all 4 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.