Abstract
Orchid seed is rather difficult to germination rather than other angiospermic seeds, because of lack radical and endosperm, leaf rudiments. Seed germination and protocorm like body (PBL) formation of Geodorum densiflorum (Lam.) Schltr. was performed. This study was conducted to determine the effects of coconut milk (CM), and BAP for optimal media culture. Seeds from sterilized capsules were cultured on two media and MS medium (Knudson C orchid medium, KnC, containing 0 and 25% (v/v) CM and 0 and 5 mgl BAP with 0.8% (w/v) agar as material. The cultures were solidifying provided by cool white florescent maintained tubes. The highest germination percentage was observation in 15% (v/v), CM and 3 mg l-1 BAP both in MS and KnC media. Seeds germinated and formed light green globular structures on the medium after three weeks of culture. These globular structures produced Protocorm-like body (PLB) and proliferated and developed into irregular-shaped rhizomes with white hairy structures. Highest in vitro seed was found in KnC medium supplemented with 15% CM and 3 mgl-1 BAP about 95.31 % whereas in MS medium maximum cultured germination KnC reached medium at 79% with 15% CM and 3 mg BAP. Overall, this study showed that Geodorum seeds culture on KnC medium containing CM and BAP can be used for clonal propagation.
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Claims 6
Claim · model-written summary
The highest germination percentage was obtained with 15% CM and 3 mg l-1 BAP in both MS and KnC media.
- Taxon
- Geodorum densiflorum
Eulophia pictae0 - Treatment
- 15% (v/v), CM and 3 mg l-1 BAPe1
- Outcome
- germination percentagee1
- Direction
- higheste1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“Seed germination and protocorm like body (PBL) formation of Geodorum densiflorum (Lam.) Schltr.”
e1“The highest germination percentage was observation in 15% (v/v), CM and 3 mg l-1 BAP both in MS and KnC media”
Claim · model-written summary
KnC medium with 15% CM and 3 mgl-1 BAP gave the highest in vitro seed germination, about 95.31%, compared with MS medium.
- Taxon
- Geodorum densiflorum
Eulophia pictae0 - Treatment
- 15% CM and 3 mgl-1 BAPe2
- Outcome
- germinatione2
- Direction
- Higheste2
- Value
- 95.31 %e2
- Qualifier
- 79%e2
- Comparator
- MS mediume2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“Seed germination and protocorm like body (PBL) formation of Geodorum densiflorum (Lam.) Schltr.”
e2“Highest in vitro seed was found in KnC medium supplemented with 15% CM and 3 mgl-1 BAP about 95.31 % whereas in MS medium maximum cultured germination KnC reached medium at 79%”
Claim · model-written summary
Seeds germinated and formed light green globular structures after three weeks of culture.
- Taxon
- Geodorum densiflorum
Eulophia pictae0 - Outcome
- light green globular structurese3
- Qualifier
- after three weeks of culturee3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“Seed germination and protocorm like body (PBL) formation of Geodorum densiflorum (Lam.) Schltr.”
e3“Seeds germinated and formed light green globular structures on the medium after three weeks of culture”
Claim · model-written summary
The globular structures produced protocorm-like bodies (PLB).
- Taxon
- Geodorum densiflorum
Eulophia pictae0 - Outcome
- Protocorm-like body (PLB)e4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“Seed germination and protocorm like body (PBL) formation of Geodorum densiflorum (Lam.) Schltr.”
e4“These globular structures produced Protocorm-like body (PLB) and proliferated and developed into irregular-shaped rhizomes with white hairy structures”
Claim · model-written summary
The protocorm-like bodies developed into irregular-shaped rhizomes with white hairy structures.
- Taxon
- Geodorum densiflorum
Eulophia pictae0 - Outcome
- irregular-shaped rhizomese4
- Qualifier
- with white hairy structurese4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“Seed germination and protocorm like body (PBL) formation of Geodorum densiflorum (Lam.) Schltr.”
e4“These globular structures produced Protocorm-like body (PLB) and proliferated and developed into irregular-shaped rhizomes with white hairy structures”
Claim · model-written summary
KnC medium containing CM and BAP can be used for clonal propagation of Geodorum densiflorum.
- Taxon
- Geodorum densiflorum
Eulophia pictae0 - Outcome
- clonal propagatione5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“Seed germination and protocorm like body (PBL) formation of Geodorum densiflorum (Lam.) Schltr.”
e5“this study showed that Geodorum seeds culture on KnC medium containing CM and BAP can be used for clonal propagation”
The same questions in other papers
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1 paper in the same direction as this paper · 1 in the opposite direction
- negative
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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.