Paper · 2015 · NBU Journal of Plant Sciences

In vitro seed germination of an Endangered Terrestrial Orchid Species Geodorum densiflorum (Lam.) Schltr.

Subhas Chandra Roy

6
claims
12
verbatim quotes
1
citations
2
research areas
✓
Open access

Abstract

Orchid seed is rather difficult to germination rather than other angiospermic seeds, because of lack radical and endosperm, leaf rudiments. Seed germination and protocorm like body (PBL) formation of Geodorum densiflorum (Lam.) Schltr. was performed. This study was conducted to determine the effects of coconut milk (CM), and BAP for optimal media culture. Seeds from sterilized capsules were cultured on two media and MS medium (Knudson C orchid medium, KnC, containing 0 and 25% (v/v) CM and 0 and 5 mgl BAP with 0.8% (w/v) agar as material. The cultures were solidifying provided by cool white florescent maintained tubes. The highest germination percentage was observation in 15% (v/v), CM and 3 mg l-1 BAP both in MS and KnC media. Seeds germinated and formed light green globular structures on the medium after three weeks of culture. These globular structures produced Protocorm-like body (PLB) and proliferated and developed into irregular-shaped rhizomes with white hairy structures. Highest in vitro seed was found in KnC medium supplemented with 15% CM and 3 mgl-1 BAP about 95.31 % whereas in MS medium maximum cultured germination KnC reached medium at 79% with 15% CM and 3 mg BAP. Overall, this study showed that Geodorum seeds culture on KnC medium containing CM and BAP can be used for clonal propagation.

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Claims 6

Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

The highest germination percentage was obtained with 15% CM and 3 mg l-1 BAP in both MS and KnC media.

Taxon
Geodorum densiflorumEulophia pictae0
Treatment
15% (v/v), CM and 3 mg l-1 BAPe1
Outcome
germination percentagee1
Direction
higheste1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Eulophia picta
Eulophia picta · Taxon · picture from its Wikimedia Commons categoryNativeplants garden · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Seed germination and protocorm like body (PBL) formation of Geodorum densiflorum (Lam.) Schltr.”

✓ located verbatim in the source · abstract, characters 140–235 · show in text ↑
e1

“The highest germination percentage was observation in 15% (v/v), CM and 3 mg l-1 BAP both in MS and KnC media”

✓ located verbatim in the source · abstract, characters 627–736 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

KnC medium with 15% CM and 3 mgl-1 BAP gave the highest in vitro seed germination, about 95.31%, compared with MS medium.

Taxon
Geodorum densiflorumEulophia pictae0
Treatment
15% CM and 3 mgl-1 BAPe2
Outcome
germinatione2
Direction
Higheste2
Value
95.31 %e2
Qualifier
79%e2
Comparator
MS mediume2

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Eulophia picta
Eulophia picta · Taxon · picture from its Wikimedia Commons categoryNativeplants garden · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Seed germination and protocorm like body (PBL) formation of Geodorum densiflorum (Lam.) Schltr.”

✓ located verbatim in the source · abstract, characters 140–235 · show in text ↑
e2

“Highest in vitro seed was found in KnC medium supplemented with 15% CM and 3 mgl-1 BAP about 95.31 % whereas in MS medium maximum cultured germination KnC reached medium at 79%”

✓ located verbatim in the source · abstract, characters 994–1170 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Seeds germinated and formed light green globular structures after three weeks of culture.

Taxon
Geodorum densiflorumEulophia pictae0
Outcome
light green globular structurese3
Qualifier
after three weeks of culturee3

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Eulophia picta
Eulophia picta · Taxon · picture from its Wikimedia Commons categoryNativeplants garden · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Seed germination and protocorm like body (PBL) formation of Geodorum densiflorum (Lam.) Schltr.”

✓ located verbatim in the source · abstract, characters 140–235 · show in text ↑
e3

“Seeds germinated and formed light green globular structures on the medium after three weeks of culture”

✓ located verbatim in the source · abstract, characters 738–840 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

The globular structures produced protocorm-like bodies (PLB).

Taxon
Geodorum densiflorumEulophia pictae0
Outcome
Protocorm-like body (PLB)e4

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Eulophia picta
Eulophia picta · Taxon · picture from its Wikimedia Commons categoryNativeplants garden · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Seed germination and protocorm like body (PBL) formation of Geodorum densiflorum (Lam.) Schltr.”

✓ located verbatim in the source · abstract, characters 140–235 · show in text ↑
e4

“These globular structures produced Protocorm-like body (PLB) and proliferated and developed into irregular-shaped rhizomes with white hairy structures”

✓ located verbatim in the source · abstract, characters 842–992 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

The protocorm-like bodies developed into irregular-shaped rhizomes with white hairy structures.

Taxon
Geodorum densiflorumEulophia pictae0
Outcome
irregular-shaped rhizomese4
Qualifier
with white hairy structurese4

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Eulophia picta
Eulophia picta · Taxon · picture from its Wikimedia Commons categoryNativeplants garden · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Seed germination and protocorm like body (PBL) formation of Geodorum densiflorum (Lam.) Schltr.”

✓ located verbatim in the source · abstract, characters 140–235 · show in text ↑
e4

“These globular structures produced Protocorm-like body (PLB) and proliferated and developed into irregular-shaped rhizomes with white hairy structures”

✓ located verbatim in the source · abstract, characters 842–992 · show in text ↑
Recommendationauthor interpretationoriginal resultbasis n/a

Claim · model-written summary

KnC medium containing CM and BAP can be used for clonal propagation of Geodorum densiflorum.

Taxon
Geodorum densiflorumEulophia pictae0
Outcome
clonal propagatione5

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Eulophia picta
Eulophia picta · Taxon · picture from its Wikimedia Commons categoryNativeplants garden · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Seed germination and protocorm like body (PBL) formation of Geodorum densiflorum (Lam.) Schltr.”

✓ located verbatim in the source · abstract, characters 140–235 · show in text ↑
e5

“this study showed that Geodorum seeds culture on KnC medium containing CM and BAP can be used for clonal propagation”

✓ located verbatim in the source · abstract, characters 1206–1322 · show in text ↑

N-benzyladenine · growth · Eulophia pictaPositive and negative results

3 papers: 2 positive, 1 negative or no effect

1 paper in the same direction as this paper · 1 in the opposite direction

  1. negative
  2. positive
See all 3 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.