Abstract
BACKGROUND: Fusarium wilt, caused by Fusarium oxysporum f. sp. cucumerinum (Foc), is a severe disease affecting cucumber (Cucumis sativus L.) production worldwide, but mechanisms underlying Fusarium wilt resistance in cucumber remain unknown. To better understand of the defense mechanisms elicited in response to Foc inoculation, RNA sequencing-based transcriptomic profiling of responses of the Fusarium wilt-resistant cucumber line 'Rijiecheng' at 0, 24, 48, 96, and 192 h after Foc inoculation was performed. RESULTS: We identified 4116 genes that were differentially expressed between 0 h and other time points after inoculation. All ethylene-related and pathogenesis-related genes from the differentially expressed genes were filtered out. Real-time PCR analysis showed that ethylene-related genes were induced in response to Foc infection. Importantly, after Foc infection and exogenous application of ethephon, a donor of ethylene, the ethylene-related genes were highly expressed. In response to exogenous ethephon treatment in conjunction with Foc inoculation, the infection resistance of cucumber seedlings was enhanced and endogenous ethylene biosynthesis increased dramatically. CONCLUSION: Collectively, ethylene signaling pathways play a positive role in regulating the defense response of cucumber to Foc infection. The results provide insight into the cucumber Fusarium wilt defense mechanisms and provide valuable information for breeding new cucumber cultivars with enhanced Fusarium wilt tolerance.
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Claims 6
Claim · model-written summary
Foc inoculation yielded 4116 differentially expressed cucumber genes.
- Taxon
- cucumber
Cucumis sativust0 - Treatment
- inoculatione0
- Outcome
- genes that were differentially expressede0
- Value
- 4116e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“We identified 4116 genes that were differentially expressed between 0 h and other time points after inoculation”
t0“cucumber (Cucumis sativus L.)”
Claim · model-written summary
Ethylene-related genes were induced after Foc infection.
- Taxon
- cucumberCucumis sativust0
- Treatment
- Foc infectione0
- Interactor
- Foce0pathogen
- Gene
- ethylene-related genese0
- Outcome
- ethylene-related genese0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ethylene-related genes were induced in response to Foc infection”
t0“cucumber (Cucumis sativus L.)”
Claim · model-written summary
Foc infection plus ethephon strongly expressed ethylene-related genes.
- Taxon
- cucumberCucumis sativust0
- Treatment
- Foc infection and exogenous application of ethephone0
- Interactor
- Foce0pathogen
- Gene
- ethylene-related genese0
- Outcome
- ethylene-related genese0
- Direction
- highly expressede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“after Foc infection and exogenous application of ethephon, a donor of ethylene, the ethylene-related genes were highly expressed”
t0“cucumber (Cucumis sativus L.)”
Claim · model-written summary
Ethephon with Foc inoculation enhanced cucumber seedling infection resistance.
- Taxon
- cucumberCucumis sativuse0
- Treatment
- exogenous ethephon treatment in conjunction with Foc inoculatione0
- Interactor
- Foce0pathogen
- Outcome
- infection resistance of cucumber seedlingse0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In response to exogenous ethephon treatment in conjunction with Foc inoculation, the infection resistance of cucumber seedlings was enhanced and endogenous ethylene biosynthesis increased dramatically”
Claim · model-written summary
Ethephon with Foc inoculation dramatically increased endogenous ethylene biosynthesis.
- Taxon
- cucumberCucumis sativuse0
- Treatment
- exogenous ethephon treatment in conjunction with Foc inoculatione0
- Interactor
- Foce0pathogen
- Outcome
- endogenous ethylene biosynthesise0
- Direction
- increased dramaticallye0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In response to exogenous ethephon treatment in conjunction with Foc inoculation, the infection resistance of cucumber seedlings was enhanced and endogenous ethylene biosynthesis increased dramatically”
Claim · model-written summary
The authors infer a positive regulatory role for ethylene signaling in cucumber defense against Foc.
- Taxon
- cucumberCucumis sativuse0
- Interactor
- Foce0pathogen
- Outcome
- defense response of cucumber to Foc infectione0
- Qualifier
- ethylene signaling pathwayse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ethylene signaling pathways play a positive role in regulating the defense response of cucumber to Foc infection”
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