Abstract
The Arabidopsis RESISTANCE TO POWDERY MILDEW 8.1 (RPW8.1) activates confined cell death and defense against different pathogens. However, the underlying regulatory mechanisms still remain elusive. Here, we show that RPW8.1 activates ethylene signaling that, in turn, negatively regulates RPW8.1 expression. RPW8.1 binds and stabilizes 1-aminocyclopropane-1-carboxylate oxidase 4 (ACO4), which may in part explain increased ethylene production and signaling in RPW8.1-expressing plants. In return, ACO4 and other key components of ethylene signaling negatively regulate RPW8.1-mediated cell death and disease resistance via suppressing RPW8.1 expression. Loss of function in ACO4, EIN2, EIN3 EIL1, ERF6, ERF016 or ORA59 increases RPW8.1-mediated cell death and defense response. By contrast, overexpression of EIN3 abolishes or significantly compromises RPW8.1-mediated cell death and disease resistance. Furthermore, ERF6, ERF016 and ORA59 appear to act as trans-repressors of RPW8.1, with OAR59 being able to directly bind to the RPW8.1 promoter. Taken together, our results have revealed a feedback regulatory circuit connecting RPW8.1 and the ethylene-signaling pathway, in which RPW8.1 enhances ethylene signaling, and the latter, in return, negatively regulates RPW8.1-mediated cell death and defense response via suppressing RPW8.1 expression to attenuate its defense activity.
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Claims 9
Claim · model-written summary
RPW8.1 activates ethylene signaling in Arabidopsis.
- Taxon
- Arabidopsis
Arabidopsist0 - Gene
- RPW8.1e0
- Outcome
- ethylene signalinge0
- Direction
- activatese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“RPW8.1 activates ethylene signaling that, in turn, negatively regulates RPW8.1 expression”
t0“Arabidopsis”
Claim · model-written summary
Ethylene signaling negatively regulates RPW8.1 expression.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- RPW8.1e0
- Outcome
- RPW8.1 expressione0
- Direction
- negatively regulatese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ethylene signaling that, in turn, negatively regulates RPW8.1 expression”
t0“Arabidopsis”
Claim · model-written summary
RPW8.1 binds ACO4.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- ACO4e0
- Outcome
- RPW8.1 bindse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“RPW8.1 binds and stabilizes 1-aminocyclopropane-1-carboxylate oxidase 4 (ACO4)”
t0“Arabidopsis”
Claim · model-written summary
RPW8.1 stabilizes ACO4.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- ACO4e0
- Outcome
- stabilizes 1-aminocyclopropane-1-carboxylate oxidase 4e0
- Direction
- stabilizese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“RPW8.1 binds and stabilizes 1-aminocyclopropane-1-carboxylate oxidase 4 (ACO4)”
t0“Arabidopsis”
Claim · model-written summary
ACO4 and ethylene signaling components suppress RPW8.1 expression, attenuating RPW8.1-mediated cell death and disease resistance.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- ACO4e0
- Outcome
- RPW8.1-mediated cell death and disease resistancee0
- Direction
- negatively regulatee0
- Qualifier
- via suppressing RPW8.1 expressione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ACO4 and other key components of ethylene signaling negatively regulate RPW8.1-mediated cell death and disease resistance via suppressing RPW8.1 expression”
t0“Arabidopsis”
Claim · model-written summary
Loss of function in the listed ethylene signaling genes increases RPW8.1-mediated cell death and defense response.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- Loss of function in ACO4, EIN2, EIN3 EIL1, ERF6, ERF016 or ORA59e0
- Outcome
- RPW8.1-mediated cell death and defense responsee0
- Direction
- increasese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Loss of function in ACO4, EIN2, EIN3 EIL1, ERF6, ERF016 or ORA59 increases RPW8.1-mediated cell death and defense response”
t0“Arabidopsis”
Claim · model-written summary
EIN3 overexpression abolishes or significantly compromises RPW8.1-mediated cell death and disease resistance.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- overexpression of EIN3e0
- Outcome
- RPW8.1-mediated cell death and disease resistancee0
- Direction
- abolishes or significantly compromisese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“overexpression of EIN3 abolishes or significantly compromises RPW8.1-mediated cell death and disease resistance”
t0“Arabidopsis”
Claim · model-written summary
ERF6, ERF016 and ORA59 appear to repress RPW8.1 transcription.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- ERF6, ERF016 and ORA59e0
- Outcome
- trans-repressors of RPW8.1e0
- Qualifier
- appeare0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ERF6, ERF016 and ORA59 appear to act as trans-repressors of RPW8.1”
t0“Arabidopsis”
Claim · model-written summary
OAR59 can directly bind the RPW8.1 promoter.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- OAR59e0
- Outcome
- directly bind to the RPW8.1 promotere0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OAR59 being able to directly bind to the RPW8.1 promoter”
t0“Arabidopsis”
The same questions in other papers
1-aminocyclopropanecarboxylate · disease · ArabidopsisResults agree
3 papers in the same direction as this paper
- positive
- positive
- positive
loss of function · disease · ArabidopsisPositive and negative results
45 papers in the same direction as this paper · 101 in the opposite direction
- negative
- negative
- negative
143 papers more in this group
See all 147 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.