Abstract
The whitefly-transmitted Tomato yellow leaf curl virus (TYLCV) is one of the most destructive viral pathogens of cultivated tomato. To combat TYLCV, resistance gene Ty-2 has been introduced into cultivated tomato (Solanum lycopersicum) from wild tomato species S. habrochaites by interspecific crossing. Introgression lines with Ty-2 contain a large inversion compared with S. lycopersicum, which causes severe suppression of recombination and has hampered the cloning of Ty-2 so far. Here, we report the fine-mapping and cloning of Ty-2 using ‘intraspecific’ crosses between a Ty-2 introgression line and susceptible S. habrochaites accessions. Ty-2 was shown to encode a nucleotide-binding leucine-rich repeat (NLR) protein. For breeding purposes, a highly specific DNA marker tightly linked to the Ty-2 gene was developed permitting marker-assisted selection. The resistance mediated by Ty-2 was effective against the Israel strain of TYLCV (TYLCV-IL) and Tomato yellow leaf curl virus-[China:Shanghai2] (TYLCV-[CN:SH2]), but not against Tomato yellow leaf curl Sardinia virus (TYLCSV) and leafhopper-transmitted Beet curly top virus (BCTV). By co-infiltration experiments we showed that transient expression of the Rep/C1 protein of TYLCV, but not of TYLCSV triggered a hypersensitive response (HR) in Nicotiana benthamiana plants co-expressing the Ty-2 gene. Our results demonstrate that the Rep/C1 gene of TYLCV-IL is the avirulence determinant of Ty-2-mediated resistance.
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Claims 10
Claim · model-written summary
Ty-2 was fine mapped and cloned using intraspecific crosses.
- Taxon
- cultivated tomato
Solanum lycopersicume1 - Gene
- Ty-2e0
- Outcome
- fine-mapping and cloning of Ty-2e0
- Comparator
- susceptible S. habrochaites accessionse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“fine-mapping and cloning of Ty-2 using ‘intraspecific’ crosses between a Ty-2 introgression line and susceptible S. habrochaites accessions”
e1“cultivated tomato (Solanum lycopersicum)”
Claim · model-written summary
Ty-2 encodes an NLR protein.
- Taxon
- cultivated tomatoSolanum lycopersicume1
- Gene
- Ty-2e0
- Outcome
- nucleotide-binding leucine-rich repeat (NLR) proteine0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Ty-2 was shown to encode a nucleotide-binding leucine-rich repeat (NLR) protein.”
e1“cultivated tomato (Solanum lycopersicum)”
Claim · model-written summary
A specific DNA marker tightly linked to Ty-2 was developed for marker-assisted selection.
- Taxon
- cultivated tomatoSolanum lycopersicume1
- Gene
- Ty-2e0
- Outcome
- marker-assisted selectione0
Not reportedComparatorValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a highly specific DNA marker tightly linked to the Ty-2 gene was developed permitting marker-assisted selection”
e1“cultivated tomato (Solanum lycopersicum)”
Claim · model-written summary
Ty-2 resistance was effective against TYLCV-IL.
- Taxon
- cultivated tomatoSolanum lycopersicume1
- Interactor
- TYLCV-ILTomato yellow leaf curl virus - Ile0pathogen
- Gene
- Ty-2e0
- Outcome
- resistance mediated by Ty-2e0
- Direction
- effectivee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“resistance mediated by Ty-2 was effective against the Israel strain of TYLCV (TYLCV-IL)”
e1“cultivated tomato (Solanum lycopersicum)”
Claim · model-written summary
Ty-2 resistance was effective against TYLCV-[CN:SH2].
- Taxon
- cultivated tomatoSolanum lycopersicume1
- Interactor
- TYLCV-[CN:SH2]e0pathogen
- Gene
- Ty-2e0
- Outcome
- resistance mediated by Ty-2e0
- Direction
- effectivee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“resistance mediated by Ty-2 was effective against the Israel strain of TYLCV (TYLCV-IL) and Tomato yellow leaf curl virus-[China:Shanghai2] (TYLCV-[CN:SH2])”
e1“cultivated tomato (Solanum lycopersicum)”
Claim · model-written summary
Ty-2 resistance was ineffective against TYLCSV.
- Taxon
- cultivated tomatoSolanum lycopersicume2
- Interactor
- TYLCSVTomato yellow leaf curl Sardinia viruse0pathogen
- Gene
- Ty-2e1
- Outcome
- resistance mediated by Ty-2e1
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“but not against Tomato yellow leaf curl Sardinia virus (TYLCSV)”
e1“resistance mediated by Ty-2 was effective against the Israel strain of TYLCV”
e2“cultivated tomato (Solanum lycopersicum)”
Claim · model-written summary
Ty-2 resistance was ineffective against BCTV.
- Taxon
- cultivated tomatoSolanum lycopersicume2
- Interactor
- BCTV
Beet curly top viruse0pathogen - Gene
- Ty-2e1
- Outcome
- resistance mediated by Ty-2e1
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“but not against Tomato yellow leaf curl Sardinia virus (TYLCSV) and leafhopper-transmitted Beet curly top virus (BCTV)”
e1“resistance mediated by Ty-2 was effective against the Israel strain of TYLCV”
e2“cultivated tomato (Solanum lycopersicum)”
Claim · model-written summary
TYLCV Rep/C1 triggered hypersensitive response in Nicotiana benthamiana expressing Ty-2.
- Taxon
- Nicotiana benthamiana
Nicotiana benthamianae0 - Treatment
- transient expression of the Rep/C1 protein of TYLCVe0
- Interactor
- TYLCVTomato yellow leaf curl viruse0pathogen
- Gene
- Ty-2e0
- Outcome
- hypersensitive response (HR)e0
- Comparator
- TYLCSVe0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“transient expression of the Rep/C1 protein of TYLCV, but not of TYLCSV triggered a hypersensitive response (HR) in Nicotiana benthamiana plants co-expressing the Ty-2 gene”
Claim · model-written summary
TYLCSV Rep/C1 did not trigger the hypersensitive response in Nicotiana benthamiana expressing Ty-2.
- Taxon
- Nicotiana benthamianaNicotiana benthamianae0
- Treatment
- TYLCSVe0
- Interactor
- TYLCSVTomato yellow leaf curl Sardinia viruse0pathogen
- Gene
- Ty-2e0
- Outcome
- hypersensitive response (HR)e0
- Comparator
- TYLCVe0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“transient expression of the Rep/C1 protein of TYLCV, but not of TYLCSV triggered a hypersensitive response (HR) in Nicotiana benthamiana plants co-expressing the Ty-2 gene”
Claim · model-written summary
TYLCV-IL Rep/C1 is the avirulence determinant for Ty-2 resistance.
- Taxon
- cultivated tomatoSolanum lycopersicume1
- Interactor
- TYLCV-ILTomato yellow leaf curl virus - Ile0pathogen
- Gene
- Ty-2e0
- Outcome
- Ty-2-mediated resistancee0
- Qualifier
- avirulence determinante0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Rep/C1 gene of TYLCV-IL is the avirulence determinant of Ty-2-mediated resistance”
e1“cultivated tomato (Solanum lycopersicum)”
The same questions in other papers
Solanum lycopersicum · Tomato yellow leaf curl virus · Nicotiana benthamianaPositive and negative results
3 papers in the same direction as this paper · 3 in the opposite direction
- negative
- negative
- negative
3 papers more in this group
See all 7 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.