Abstract
The objective of the present study was to develop a cost-effective medium, using agro-industrial wastes for the production of a polygalacturonase by Wickerhanomyces anomalus of interest in cassava starch industries. The effect of several raw agro-industrial wastes and others nutrients on polygalacturonase production by W. anomalus, were evaluated, in a reference fermentation medium, using statistical designs, by batch culture. The ability of the cell-free supernatant to extract cassava starch was evaluated. Lemon peel was the best inducer for the production of PGase. Statistical analysis of the data showed that lemon peel, Mg+2 and PO4HK2 had significant effect on PGase production, and the others variables (yeast extract, Ca+2, Fe+2, amino acid and trace element solution) were no significant. PGase synthesis reached ~31 EUmL-1, in the OFM (glucose, lemon peel, urea, vitamins, KH2PO4 and MgSO4), after 12 h of culture, at a lab scale bioreactor. PGase of W. anomalus, was able to disintegrate cassava tuber tissue, and the starch granules contained within the cells were released into the reaction medium. Lemon peel can be used as inducer for PGase production by W. anomalus, in a low cost culture medium, appropriate for the production of the enzyme at large scale.
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Claims 13
Claim · model-written summary
Lemon peel was the best inducer of PGase production by Wickerhanomyces anomalus among the raw agro-industrial wastes tested.
- Taxon
- cassava
Manihot esculentae0 - Treatment
- Lemon peele1
- Outcome
- production of PGasee1
- Direction
- beste1
- Comparator
- several raw agro-industrial wastese7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“of interest in cassava starch industries”
e1“Lemon peel was the best inducer for the production of PGase”
e7“The effect of several raw agro-industrial wastes and others nutrients on polygalacturonase production”
Claim · model-written summary
Lemon peel had a significant effect on PGase production by Wickerhanomyces anomalus.
- Taxon
- cassavaManihot esculentae0
- Treatment
- lemon peele2
- Outcome
- PGase productione2
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“of interest in cassava starch industries”
e2“lemon peel, Mg+2 and PO4HK2 had significant effect on PGase production”
Claim · model-written summary
Mg+2 had a significant effect on PGase production by Wickerhanomyces anomalus.
- Taxon
- cassavaManihot esculentae0
- Treatment
- Mg+2e2
- Outcome
- PGase productione2
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“of interest in cassava starch industries”
e2“lemon peel, Mg+2 and PO4HK2 had significant effect on PGase production”
Claim · model-written summary
PO4HK2 had a significant effect on PGase production by Wickerhanomyces anomalus.
- Taxon
- cassavaManihot esculentae0
- Treatment
- PO4HK2e2
- Outcome
- PGase productione2
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“of interest in cassava starch industries”
e2“lemon peel, Mg+2 and PO4HK2 had significant effect on PGase production”
Claim · model-written summary
Yeast extract had no significant effect on PGase production.
- Taxon
- cassavaManihot esculentae0
- Treatment
- yeast extracte3
- Outcome
- PGase productione2
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“of interest in cassava starch industries”
e3“the others variables (yeast extract, Ca+2, Fe+2, amino acid and trace element solution) were no significant”
e2“lemon peel, Mg+2 and PO4HK2 had significant effect on PGase production”
Claim · model-written summary
Ca+2 had no significant effect on PGase production.
- Taxon
- cassavaManihot esculentae0
- Treatment
- Ca+2e3
- Outcome
- PGase productione2
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“of interest in cassava starch industries”
e3“the others variables (yeast extract, Ca+2, Fe+2, amino acid and trace element solution) were no significant”
e2“lemon peel, Mg+2 and PO4HK2 had significant effect on PGase production”
Claim · model-written summary
Fe+2 had no significant effect on PGase production.
- Taxon
- cassavaManihot esculentae0
- Treatment
- Fe+2e3
- Outcome
- PGase productione2
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“of interest in cassava starch industries”
e3“the others variables (yeast extract, Ca+2, Fe+2, amino acid and trace element solution) were no significant”
e2“lemon peel, Mg+2 and PO4HK2 had significant effect on PGase production”
Claim · model-written summary
Amino acid had no significant effect on PGase production.
- Taxon
- cassavaManihot esculentae0
- Treatment
- amino acide3
- Outcome
- PGase productione2
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“of interest in cassava starch industries”
e3“the others variables (yeast extract, Ca+2, Fe+2, amino acid and trace element solution) were no significant”
e2“lemon peel, Mg+2 and PO4HK2 had significant effect on PGase production”
Claim · model-written summary
The trace element solution had no significant effect on PGase production.
- Taxon
- cassavaManihot esculentae0
- Treatment
- trace element solutione3
- Outcome
- PGase productione2
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“of interest in cassava starch industries”
e3“the others variables (yeast extract, Ca+2, Fe+2, amino acid and trace element solution) were no significant”
e2“lemon peel, Mg+2 and PO4HK2 had significant effect on PGase production”
Claim · model-written summary
PGase synthesis reached about 31 EUmL-1 in the OFM medium after 12 h of culture in a lab-scale bioreactor.
- Taxon
- cassavaManihot esculentae0
- Outcome
- PGase synthesise4
- Value
- ~31 EUmL-1e4
- Setting
- lab scale bioreactore4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“of interest in cassava starch industries”
e4“PGase synthesis reached ~31 EUmL-1, in the OFM (glucose, lemon peel, urea, vitamins, KH2PO4 and MgSO4), after 12 h of culture, at a lab scale bioreactor”
Claim · model-written summary
The PGase of Wickerhanomyces anomalus disintegrated cassava tuber tissue.
Verbatim
e5“PGase of W. anomalus, was able to disintegrate cassava tuber tissue, and the starch granules contained within the cells were released into the reaction medium”
Claim · model-written summary
Starch granules contained within cassava tuber cells were released into the reaction medium by the PGase of Wickerhanomyces anomalus.
Verbatim
e5“PGase of W. anomalus, was able to disintegrate cassava tuber tissue, and the starch granules contained within the cells were released into the reaction medium”
Claim · model-written summary
Lemon peel can be used as an inducer of PGase production by Wickerhanomyces anomalus in a low-cost culture medium suitable for large-scale enzyme production.
- Taxon
- cassavaManihot esculentae0
- Treatment
- Lemon peele6
- Outcome
- PGase productione6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“of interest in cassava starch industries”
e6“Lemon peel can be used as inducer for PGase production by W. anomalus, in a low cost culture medium, appropriate for the production of the enzyme at large scale”
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