Abstract
The farnesyltransferase -subunit (FTA) may be involved in the regulation of defence responses against pathogens in plants. In this study, this gene was amplified from Catharanthus roseus (CrFTA gene). The cDNA was found to be 1 403 bp long, and encodes a putative protein of 331 amino acids that contains a conserved PPTA motif. The phylogenetic analysis showed that the sequence of CrFTA is the most similar to that from Coffea canephora. The qRT-PCR assays indicated that CrFTA is expressed in the leaves, stems, and roots. During a Candidatus Liberibacter asiaticus (Ca. L. asiaticus) infection, the CrFTA expression levels significantly increased and reached 18-fold that measured in the control group, after which its expression decreased gradually from 22 days after top-grafting (DAT) to the end of the experiment. Spray application of Manumycin A (ManuA), a specific inhibitor of farnesyltransferase, on the leaves of C. roseus plants caused a significant decrease in the CrFTA expression and a significant increase in the Ca. L. asiaticus positivity percentage after top-grafting with the Ca. L. asiaticus-infected shoots compared with the groups not treated with ManuA. Furthermore, ABA had no significant effect on the relative expression of CrFTA and the number of Ca. L. asiaticus-positive plants. These results suggest that CrFTA most likely plays a role in mediating the tolerance to a Ca. L. asiaticus infection in C. roseus.
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Claims 11
Claim · model-written summary
CrFTA is expressed in the leaves, stems and roots of Catharanthus roseus.
- Taxon
- Catharanthus roseus
Catharanthus roseust0 - Organ / stage
- leaves, stems, and rootse1
- Gene
- CrFTAe1
- Outcome
- expressede1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“the Catharanthus roseus farnesyltransferase α-subunit gene”
e1“The qRT-PCR assays indicated that CrFTA is expressed in the leaves, stems, and roots”
Claim · model-written summary
CrFTA expression increased 18-fold during Ca. L. asiaticus infection relative to controls.
- Taxon
- Catharanthus roseusCatharanthus roseust0
- Treatment
- Candidatus Liberibacter asiaticus (Ca. L. asiaticus) infectione2
- Interactor
- Candidatus Liberibacter asiaticus
Candidatus Liberibacter asiaticuse2pathogen - Gene
- CrFTAe2
- Outcome
- CrFTA expression levelse2
- Direction
- increasede2
- Value
- 18-folde2
- Comparator
- control groupe2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“the Catharanthus roseus farnesyltransferase α-subunit gene”
e2“During a Candidatus Liberibacter asiaticus (Ca. L. asiaticus) infection, the CrFTA expression levels significantly increased and reached 18-fold that measured in the control group”
Claim · model-written summary
CrFTA expression decreased gradually from 22 days after top-grafting to the end of the experiment.
- Taxon
- Catharanthus roseusCatharanthus roseust0
- Treatment
- 22 days after top-grafting (DAT)e3
- Gene
- CrFTAe2
- Outcome
- expressione3
- Direction
- decreasede3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“the Catharanthus roseus farnesyltransferase α-subunit gene”
e2“During a Candidatus Liberibacter asiaticus (Ca. L. asiaticus) infection, the CrFTA expression levels significantly increased and reached 18-fold that measured in the control group”
e3“after which its expression decreased gradually from 22 days after top-grafting (DAT) to the end of the experiment”
Claim · model-written summary
Spray application of Manumycin A decreased CrFTA expression compared with groups not treated with it.
- Taxon
- Catharanthus roseusCatharanthus roseust0
- Treatment
- Spray application of Manumycin A (ManuA)e0
- Gene
- CrFTAe0
- Outcome
- CrFTA expressione0
- Direction
- decreasee0
- Comparator
- groups not treated with ManuAe4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“the Catharanthus roseus farnesyltransferase α-subunit gene”
e0“Spray application of Manumycin A (ManuA), a specific inhibitor of farnesyltransferase, on the leaves of C. roseus plants caused a significant decrease in the CrFTA expression”
e4“a significant increase in the Ca. L. asiaticus positivity percentage after top-grafting with the Ca. L. asiaticus-infected shoots compared with the groups not treated with ManuA”
Claim · model-written summary
Spray application of Manumycin A increased the Ca. L. asiaticus positivity percentage compared with groups not treated with it.
- Taxon
- Catharanthus roseusCatharanthus roseust0
- Treatment
- Spray application of Manumycin A (ManuA)e0
- Interactor
- Ca. L. asiaticuse4pathogen
- Outcome
- Ca. L. asiaticus positivity percentagee4
- Direction
- increasee4
- Comparator
- groups not treated with ManuAe4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“the Catharanthus roseus farnesyltransferase α-subunit gene”
e0“Spray application of Manumycin A (ManuA), a specific inhibitor of farnesyltransferase, on the leaves of C. roseus plants caused a significant decrease in the CrFTA expression”
e4“a significant increase in the Ca. L. asiaticus positivity percentage after top-grafting with the Ca. L. asiaticus-infected shoots compared with the groups not treated with ManuA”
Claim · model-written summary
ABA had no significant effect on the relative expression of CrFTA.
- Taxon
- Catharanthus roseusCatharanthus roseust0
- Treatment
- ABAe5
- Gene
- CrFTAe5
- Outcome
- relative expression of CrFTAe5
- Direction
- no_changeinferrede5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“the Catharanthus roseus farnesyltransferase α-subunit gene”
e5“ABA had no significant effect on the relative expression of CrFTA and the number of Ca. L. asiaticus-positive plants”
Claim · model-written summary
ABA had no significant effect on the number of Ca. L. asiaticus-positive plants.
- Taxon
- Catharanthus roseusCatharanthus roseust0
- Treatment
- ABAe5
- Interactor
- Ca. L. asiaticuse5pathogen
- Outcome
- number of Ca. L. asiaticus-positive plantse5
- Direction
- no_changeinferrede5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“the Catharanthus roseus farnesyltransferase α-subunit gene”
e5“ABA had no significant effect on the relative expression of CrFTA and the number of Ca. L. asiaticus-positive plants”
Claim · model-written summary
CrFTA most likely mediates tolerance to Ca. L. asiaticus infection in Catharanthus roseus.
- Taxon
- Catharanthus roseusCatharanthus roseust0
- Interactor
- Ca. L. asiaticuse6pathogen
- Gene
- CrFTAe6
- Outcome
- tolerance to a Ca. L. asiaticus infectione6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“the Catharanthus roseus farnesyltransferase α-subunit gene”
e6“These results suggest that CrFTA most likely plays a role in mediating the tolerance to a Ca. L. asiaticus infection in C. roseus”
Claim · model-written summary
The CrFTA sequence is most similar to the Coffea canephora sequence.
- Taxon
- Catharanthus roseusCatharanthus roseust0
- Gene
- CrFTAe7
- Outcome
- the sequence of CrFTA is the most similare7
- Qualifier
- to that from Coffea canephorae7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“the Catharanthus roseus farnesyltransferase α-subunit gene”
e7“The phylogenetic analysis showed that the sequence of CrFTA is the most similar to that from Coffea canephora”
Claim · model-written summary
The CrFTA cDNA is 1 403 bp long.
- Taxon
- Catharanthus roseusCatharanthus roseust0
- Outcome
- cDNAe0
- Value
- 1 403 bpe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“the Catharanthus roseus farnesyltransferase α-subunit gene”
e0“The cDNA was found to be 1 403 bp long, and encodes a putative protein of 331 amino acids that contains a conserved PPTA motif”
Claim · model-written summary
The CrFTA putative protein has 331 amino acids and contains a conserved PPTA motif.
- Taxon
- Catharanthus roseusCatharanthus roseust0
- Outcome
- putative proteine0
- Value
- 331 amino acidse0
- Qualifier
- contains a conserved PPTA motife0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“the Catharanthus roseus farnesyltransferase α-subunit gene”
e0“The cDNA was found to be 1 403 bp long, and encodes a putative protein of 331 amino acids that contains a conserved PPTA motif”
The same questions in other papers
(+)-abscisic acid · molecular: expression · Catharanthus roseus (Madagascar periwinkle)Positive and negative results
1 in the opposite direction
- positive
application · nutrient: content · Catharanthus roseus (Madagascar periwinkle)Positive and negative results
2 in the opposite direction
- positive
- positive
infection · molecular: expression · Catharanthus roseus (Madagascar periwinkle)Results agree
1 paper in the same direction as this paper
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.