Abstract
Cell death is the ultimate end of a cell cycle that occurs in all living organisms during development or responses to biotic and abiotic stresses. In the course of evolution, plants and animals evolve various molecular mechanisms to regulate cell death; however, some of them are conserved among both these kingdoms. It was found that mammalian proapoptotic BCL-2 associated X (Bax) protein, when expressed in plants, induces cell death, similar to hypersensitive response (HR). It was also shown that changes in the expression level of genes encoding proteins involved in stress response or oxidative status regulation mitigate Bax-induced plant cell death. In our study, we focused on the evolutional compatibility of animal and plant cell death molecular mechanisms. Therefore, we studied the deregulation of reactive oxygen species burst and HR-like propagation in Arabidopsis thaliana expressing mammalian Bax. We were able to diminish Bax-induced oxidative stress and HR progression through the genetic cross with plants mutated in ENHANCED DISEASE SUSCEPTIBILITY 1 (EDS1), which is a plant-positive HR regulator. Plants expressing the mouse Bax gene in eds1-1 null mutant background demonstrated less pronounced cell death and exhibited higher antioxidant system efficiency compared to Bax-expressing plants. Moreover, eds1/Bax plants did not show HR marker genes induction, as in the case of the Bax-expressing line. The present study indicates some common molecular features between animal and plant cell death regulation and can be useful to better understand the evolution of cell death mechanisms in plants and animals.
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Claims 5
Claim · model-written summary
Bax expression in the eds1-1 mutant background gave less pronounced cell death than in Bax-expressing plants.
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianae3 - Treatment
- mouse Bax gene in eds1-1 null mutant backgrounde1
- Gene
- mouse Baxe1
- Outcome
- cell deathe1
- Direction
- less pronouncede1
- Comparator
- Bax-expressing plantse2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e3“Arabidopsis thaliana expressing mammalian Bax”
e1“Plants expressing the mouse Bax gene in eds1-1 null mutant background demonstrated less pronounced cell death”
e2“exhibited higher antioxidant system efficiency compared to Bax-expressing plants”
Claim · model-written summary
Bax expression in the eds1-1 mutant background gave higher antioxidant system efficiency than in Bax-expressing plants.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae3
- Treatment
- mouse Bax gene in eds1-1 null mutant backgrounde1
- Outcome
- antioxidant system efficiencye2
- Direction
- highere2
- Comparator
- Bax-expressing plantse2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e3“Arabidopsis thaliana expressing mammalian Bax”
e1“Plants expressing the mouse Bax gene in eds1-1 null mutant background demonstrated less pronounced cell death”
e2“exhibited higher antioxidant system efficiency compared to Bax-expressing plants”
Claim · model-written summary
eds1/Bax plants did not show HR marker gene induction, unlike the Bax-expressing line.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae3
- Treatment
- eds1/Baxe4
- Outcome
- HR marker genes inductione4
- Qualifier
- did not showe4
- Comparator
- Bax-expressing linee4
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e3“Arabidopsis thaliana expressing mammalian Bax”
e4“eds1/Bax plants did not show HR marker genes induction, as in the case of the Bax-expressing line”
Claim · model-written summary
The eds1 genetic cross diminished Bax-induced oxidative stress and HR progression.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae3
- Treatment
- genetic cross with plants mutated in ENHANCED DISEASE SUSCEPTIBILITY 1 (EDS1)e5
- Gene
- EDS1e5
- Outcome
- Bax-induced oxidative stress and HR progressione5
- Direction
- diminishe5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e3“Arabidopsis thaliana expressing mammalian Bax”
e5“We were able to diminish Bax-induced oxidative stress and HR progression through the genetic cross with plants mutated in ENHANCED DISEASE SUSCEPTIBILITY 1 (EDS1)”
Claim · model-written summary
The study indicates common molecular features of animal and plant cell death regulation.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae3
- Outcome
- cell death regulatione6
- Qualifier
- common molecular features between animal and plante6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e3“Arabidopsis thaliana expressing mammalian Bax”
e6“The present study indicates some common molecular features between animal and plant cell death regulation”
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