Paper · 2019 · Applied Sciences

Fermentation Characteristics of Lactobacillus Plantarum and Pediococcus Species Isolated from Sweet Sorghum Silage and Their Application as Silage Inoculants

Hager Alhaag; Xianjun Yuan; Azizza Mala; et al.

10
claims
20
verbatim quotes
58
citations
2
research areas
✓
Open access · cc-by

Abstract

This study aims to evaluate the fermentation characteristics of Lactobacillus plantarum and Pediococcus spp isolated from sweet sorghum silage to enhance the fermentation quality of Napier grass and sweet sorghum silage. Based on molecular 16S ribosomal ribonucleic identification the isolated strains were phylogenetically related to Lactobacillus plantarum (HY1), Pediococcus acidilactici (HY2) and Pediococcus claussenii (HY3). Strains HY1, HY2 and HY3 and commercial bacteria Lactobacillus plantarum, Ecosyl; (MTD\1( were ensiled with sweet sorghum and Napier grass and the non-inoculated grasses, have been arranged in a completely randomized experimental design in a 5 (inoculants) × 3 (ensiling periods). In both grasses, the fermentation characteristics chemical composition and microbial population were assessed at 5–30 and 90 days of ensiling. The results showed that the effect of addition inoculants significantly reduced (p < 0.05) the pH, ammonia-N, acetic acid and undesirable microbial population and increased (p < 0.05) lactic acid and lactic acid bacteria counting when compared to the control. The effect of ensiling days on silage quality through the increasing lactic acid, acetic acid, ammonia-N, propionic acid and butyric acid whereas decreasing pH and water-soluble carbohydrates and microbial counts. In both sweet sorghum and Napier silage treated with isolated strains showed the best results in silage quality. The HY3 belongs to Pediococcus claussenii was not extensively studied in silage but it has shown good fermentation quality which strongly recommended to apply as probiotic.

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Claims 10

Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Inoculants significantly reduced silage pH, ammonia-N and acetic acid compared with the control.

Taxon
Sweet Sorghumt0
Treatment
addition inoculantse2
Outcome
pH, ammonia-N, acetic acide2
Direction
reducede2
Comparator
the controle2

Verbatim

t0

“Sweet Sorghum Silage”

✓ located verbatim in the source · title, characters 94–114 · show in text ↑
e2

“The results showed that the effect of addition inoculants significantly reduced (p < 0.05) the pH, ammonia-N, acetic acid and undesirable microbial population and increased (p < 0.05) lactic acid and lactic acid bacteria counting when compared to the control”

✓ located verbatim in the source · abstract, characters 855–1113 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Inoculants significantly reduced the undesirable microbial population in silage compared with the control.

Taxon
Sweet Sorghumt0
Treatment
addition inoculantse2
Outcome
undesirable microbial populatione2
Direction
reducede2
Comparator
the controle2

Verbatim

t0

“Sweet Sorghum Silage”

✓ located verbatim in the source · title, characters 94–114 · show in text ↑
e2

“The results showed that the effect of addition inoculants significantly reduced (p < 0.05) the pH, ammonia-N, acetic acid and undesirable microbial population and increased (p < 0.05) lactic acid and lactic acid bacteria counting when compared to the control”

✓ located verbatim in the source · abstract, characters 855–1113 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Inoculants significantly increased lactic acid and lactic acid bacteria counts compared with the control.

Taxon
Sweet Sorghumt0
Treatment
addition inoculantse2
Outcome
lactic acid and lactic acid bacteria countinge2
Direction
increasede2
Comparator
the controle2

Verbatim

t0

“Sweet Sorghum Silage”

✓ located verbatim in the source · title, characters 94–114 · show in text ↑
e2

“The results showed that the effect of addition inoculants significantly reduced (p < 0.05) the pH, ammonia-N, acetic acid and undesirable microbial population and increased (p < 0.05) lactic acid and lactic acid bacteria counting when compared to the control”

✓ located verbatim in the source · abstract, characters 855–1113 · show in text ↑
Associationobservedoriginal resultmeasured

Claim · model-written summary

Ensiling days increased lactic acid, acetic acid and ammonia-N in silage.

Taxon
Sweet Sorghumt0
Treatment
ensiling dayse3
Outcome
lactic acid, acetic acid, ammonia-Ne3
Direction
increasinge3

Verbatim

t0

“Sweet Sorghum Silage”

✓ located verbatim in the source · title, characters 94–114 · show in text ↑
e3

“The effect of ensiling days on silage quality through the increasing lactic acid, acetic acid, ammonia-N, propionic acid and butyric acid whereas decreasing pH and water-soluble carbohydrates and microbial counts”

✓ located verbatim in the source · abstract, characters 1115–1327 · show in text ↑
Associationobservedoriginal resultmeasured

Claim · model-written summary

Ensiling days increased propionic acid and butyric acid in silage.

Taxon
Sweet Sorghumt0
Treatment
ensiling dayse3
Outcome
propionic acid and butyric acide3
Direction
increasinge3

Verbatim

t0

“Sweet Sorghum Silage”

✓ located verbatim in the source · title, characters 94–114 · show in text ↑
e3

“The effect of ensiling days on silage quality through the increasing lactic acid, acetic acid, ammonia-N, propionic acid and butyric acid whereas decreasing pH and water-soluble carbohydrates and microbial counts”

✓ located verbatim in the source · abstract, characters 1115–1327 · show in text ↑
Associationobservedoriginal resultmeasured

Claim · model-written summary

Ensiling days decreased silage pH, water-soluble carbohydrates and microbial counts.

Taxon
Sweet Sorghumt0
Treatment
ensiling dayse3
Outcome
pH and water-soluble carbohydrates and microbial countse3
Direction
decreasinge3

Verbatim

t0

“Sweet Sorghum Silage”

✓ located verbatim in the source · title, characters 94–114 · show in text ↑
e3

“The effect of ensiling days on silage quality through the increasing lactic acid, acetic acid, ammonia-N, propionic acid and butyric acid whereas decreasing pH and water-soluble carbohydrates and microbial counts”

✓ located verbatim in the source · abstract, characters 1115–1327 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Silage of sweet sorghum treated with the isolated strains gave the best silage quality.

Taxon
sweet sorghume4
Treatment
isolated strainse4
Outcome
silage qualitye4
Direction
best resultse4

Not reportedComparator

Verbatim

t0

“Sweet Sorghum Silage”

✓ located verbatim in the source · title, characters 94–114 · show in text ↑
e4

“In both sweet sorghum and Napier silage treated with isolated strains showed the best results in silage quality”

✓ located verbatim in the source · abstract, characters 1329–1440 · show in text ↑
Recommendationauthor interpretationoriginal resultbasis n/a

Claim · model-written summary

Pediococcus claussenii HY3 is recommended for application as a probiotic in silage.

Taxon
Sweet Sorghumt0
Material
HY3e5
Outcome
apply as probiotice5

Verbatim

t0

“Sweet Sorghum Silage”

✓ located verbatim in the source · title, characters 94–114 · show in text ↑
e5

“The HY3 belongs to Pediococcus claussenii was not extensively studied in silage but it has shown good fermentation quality which strongly recommended to apply as probiotic”

✓ located verbatim in the source · abstract, characters 1442–1613 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

The HY3 strain showed good fermentation quality in silage.

Taxon
Sweet Sorghumt0
Material
HY3e5
Outcome
good fermentation qualitye5

Verbatim

t0

“Sweet Sorghum Silage”

✓ located verbatim in the source · title, characters 94–114 · show in text ↑
e5

“The HY3 belongs to Pediococcus claussenii was not extensively studied in silage but it has shown good fermentation quality which strongly recommended to apply as probiotic”

✓ located verbatim in the source · abstract, characters 1442–1613 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

The isolated strains were phylogenetically related to Lactobacillus plantarum (HY1), Pediococcus acidilactici (HY2) and Pediococcus claussenii (HY3).

Taxon
Sweet Sorghumt0
Material
HY1e6
Outcome
phylogenetically related to Lactobacillus plantarume6

Verbatim

t0

“Sweet Sorghum Silage”

✓ located verbatim in the source · title, characters 94–114 · show in text ↑
e6

“the isolated strains were phylogenetically related to Lactobacillus plantarum (HY1), Pediococcus acidilactici (HY2) and Pediococcus claussenii (HY3)”

✓ located verbatim in the source · abstract, characters 281–429 · show in text ↑

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inoculation · soil: biology · SorghumPositive and negative results

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inoculation · soil: chemistry · SorghumPositive and negative results

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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.