Abstract
BACKGROUND: The basic helix-loop-helix (bHLH) proteins, a large transcription factors family, are involved in plant growth and development, and defensive response to various environmental stresses. The resurrection plant Myrothamnus flabellifolia is known for its extremely strong drought tolerance, but few bHLHs taking part in abiotic stress response have been unveiled in M. flabellifolia. RESULTS: In the present research, we cloned and characterized a dehydration-inducible gene, MfbHLH38, from M. flabellifolia. The MfbHLH38 protein is localized in the nucleus, where it may act as a transcription factor. Heterologous expression of MfbHLH38 in Arabidopsis improved the tolerance to drought and salinity stresses, as determined by the studies on physiological indexes, such as contents of chlorophyll, malondialdehyde (MDA), proline (Pro), soluble protein, and soluble sugar, water loss rate of detached leaves, reactive oxygen species (ROS) accumulation, as well as antioxidant enzyme activities. Besides, MfbHLH38 overexpression increased the sensitivity of stomatal closure to mannitol and abscisic acid (ABA), improved ABA level under drought stress, and elevated the expression of genes associated with ABA biosynthesis and ABA responding, sucha as NCED3, P5CS, and RD29A. CONCLUSIONS: Our results presented evidence that MfbHLH38 enhanced tolerance to drought and salinity stresses in Arabidopsis through increasing water retention ability, regulating osmotic balance, decreasing stress-induced oxidation damage, and possibly participated in ABA-dependent stress-responding pathway.
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Claims 6
Claim · model-written summary
MfbHLH38 was cloned and characterized as a dehydration-inducible gene from M. flabellifolia.
Verbatim
e0“In the present research, we cloned and characterized a dehydration-inducible gene, MfbHLH38, from M. flabellifolia.”
Claim · model-written summary
MfbHLH38 protein localized in the nucleus.
Verbatim
e0“The MfbHLH38 protein is localized in the nucleus, where it may act as a transcription factor.”
t0“Myrothamnus flabellifolia”
Claim · model-written summary
MfbHLH38 expression improved Arabidopsis tolerance to drought and salinity.
- Taxon
- Arabidopsis
Arabidopsise0 - Treatment
- Heterologous expression of MfbHLH38e0
- Gene
- MfbHLH38e0
- Outcome
- tolerance to drought and salinity stressese0
- Direction
- improvede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Heterologous expression of MfbHLH38 in Arabidopsis improved the tolerance to drought and salinity stresses, as determined by the studies on physiological indexes, such as contents of chlorophyll, malondialdehyde (MDA), proline (Pro), soluble protein, and soluble sugar, water loss rate of detached leaves, reactive oxygen species (ROS) accumulation, as well as antioxidant enzyme activities.”
Claim · model-written summary
MfbHLH38 overexpression increased sensitivity of stomatal closure in Arabidopsis.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- MfbHLH38 overexpressione0
- Gene
- MfbHLH38e0
- Outcome
- sensitivity of stomatal closuree0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Besides, MfbHLH38 overexpression increased the sensitivity of stomatal closure to mannitol and abscisic acid (ABA), improved ABA level under drought stress, and elevated the expression of genes associated with ABA biosynthesis and ABA responding, sucha as NCED3, P5CS, and RD29A.”
t0“Arabidopsis”
Claim · model-written summary
MfbHLH38 overexpression improved ABA level in Arabidopsis.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- MfbHLH38 overexpressione0
- Gene
- MfbHLH38e0
- Outcome
- ABA levele0
- Direction
- improvede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Besides, MfbHLH38 overexpression increased the sensitivity of stomatal closure to mannitol and abscisic acid (ABA), improved ABA level under drought stress, and elevated the expression of genes associated with ABA biosynthesis and ABA responding, sucha as NCED3, P5CS, and RD29A.”
t0“Arabidopsis”
Claim · model-written summary
MfbHLH38 overexpression elevated expression of genes associated with ABA biosynthesis and ABA responding in Arabidopsis.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- MfbHLH38 overexpressione0
- Gene
- MfbHLH38e0
- Outcome
- expression of genes associated with ABA biosynthesis and ABA respondinge0
- Direction
- elevatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Besides, MfbHLH38 overexpression increased the sensitivity of stomatal closure to mannitol and abscisic acid (ABA), improved ABA level under drought stress, and elevated the expression of genes associated with ABA biosynthesis and ABA responding, sucha as NCED3, P5CS, and RD29A.”
t0“Arabidopsis”
The same questions in other papers
overexpression · disease · ArabidopsisPositive and negative results
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