Abstract
[Objective]The present experiment was aimed to study the effects of different hormone combinations on callus induction in anthers taken from tomato thermo-sensitive genic male sterile lines(TSGMSLs) and their hybrid combinations.[Method]Tomato TSGMSLs,viz.,Da 107 and Di D4,and their hybrid combinations,viz.,Da 107×Niufanqie and Di D4×Hongkunlun were used in the studies.Combinations of different hormones(CPPU,TDZ,KT) in basic MS medium(containing 1.0 mg/L 2,4-D+1.0 mg/L 6-BA) were used for anther callus induction.[Result]Adding different CPPU,TDZ or KT concentrations in basic MS medium significantly improved the induction of anther callus,and the suitable concentration of CPPU,TDZ and KT was 0.4-0.6,0.03 and 1.5 mg/L,respectively.Under the same hormone combination conditions,the anthers taken from tomato TSGMSLs showed better callus induction compared to those taken from hybrid combinations,the order of the average rate of callus induction and average diameter of callus was:Di D4Da 107Di D4×HongkunlunDa 107×Niufanqie.[Conclusion]Anthers of different tomato genotypes differed in their sensitivities to different hormones.Furthermore,different combinations of hormones also differed in affecting induction of tomato anther callus,out of them,the combinations of KT and 2,4-D+6-BA showed best results.
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Claims 7
Claim · model-written summary
Adding different CPPU, TDZ or KT concentrations to basic MS medium significantly improved anther callus induction.
- Taxon
- tomato
Solanum lycopersicumt0 - Treatment
- CPPU,TDZ or KT concentrationse0
- Outcome
- induction of anther calluse0
- Direction
- improvede0
- Comparator
- basic MS mediume0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“tomato”
e0“Adding different CPPU,TDZ or KT concentrations in basic MS medium significantly improved the induction of anther callus”
Claim · model-written summary
The suitable CPPU concentration was 0.4-0.6 mg/L.
- Taxon
- tomatoSolanum lycopersicumt0
- Treatment
- CPPUe0
- Outcome
- suitable concentratione0
- Value
- 0.4-0.6e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“tomato”
e0“the suitable concentration of CPPU,TDZ and KT was 0.4-0.6,0.03 and 1.5 mg/L,respectively”
Claim · model-written summary
The suitable TDZ concentration was 0.03 mg/L.
- Taxon
- tomatoSolanum lycopersicumt0
- Treatment
- TDZe0
- Outcome
- suitable concentratione0
- Value
- 0.03e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“tomato”
e0“the suitable concentration of CPPU,TDZ and KT was 0.4-0.6,0.03 and 1.5 mg/L,respectively”
Claim · model-written summary
The suitable KT concentration was 1.5 mg/L.
- Taxon
- tomatoSolanum lycopersicumt0
- Treatment
- KTe0
- Outcome
- suitable concentratione0
- Value
- 1.5 mg/Le0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“tomato”
e0“the suitable concentration of CPPU,TDZ and KT was 0.4-0.6,0.03 and 1.5 mg/L,respectively”
Claim · model-written summary
Anthers from tomato TSGMSLs showed better callus induction than those from hybrid combinations under the same hormone conditions.
- Taxon
- tomatoSolanum lycopersicumt0
- Material
- tomato TSGMSLse0
- Outcome
- callus inductione0
- Direction
- bettere0
- Comparator
- hybrid combinationse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“tomato”
e0“the anthers taken from tomato TSGMSLs showed better callus induction compared to those taken from hybrid combinations”
Claim · model-written summary
An order of the genotypes for average callus induction rate and callus diameter is given (Di D4, Da 107, Di D4×Hongkunlun, Da 107×Niufanqie as listed; ordering symbols are missing in the source).
- Taxon
- tomatoSolanum lycopersicumt0
- Outcome
- average rate of callus induction and average diameter of calluse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Schema note from the model: ordering symbols lost in the source text
Verbatim
t0“tomato”
e0“the order of the average rate of callus induction and average diameter of callus was:Di D4Da 107Di D4×HongkunlunDa 107×Niufanqie”
Claim · model-written summary
Hormone combinations differed in their effect on anther callus induction, and KT with 2,4-D+6-BA gave the best results.
- Taxon
- tomatoSolanum lycopersicumt0
- Treatment
- combinations of KT and 2,4-D+6-BAe0
- Outcome
- induction of tomato anther calluse0
- Direction
- best resultse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“tomato”
e0“different combinations of hormones also differed in affecting induction of tomato anther callus,out of them,the combinations of KT and 2,4-D+6-BA showed best results”
The same questions in other papers
2,4-D · molecular: expression · Solanum lycopersicum (tomato)Positive and negative results
1 paper in the same direction as this paper · 1 in the opposite direction
- no effect
- positive
kinetin · molecular: expression · Solanum lycopersicum (tomato)Results agree
1 paper in the same direction as this paper
- positive
N-benzyladenine · molecular: expression · Solanum lycopersicum (tomato)Results agree
2 papers in the same direction as this paper
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.