Paper · 2020 · Rural Sustainability Research

Influence of Germination Conditions and Parameters on the Content of Total Phenolic Compounds and Scavenging Activity in Germinated Seeds

Ilze Bernate; Martins Sabovics; Lolita Tomsone

4
claims
8
verbatim quotes
4
citations
1
research areas
✓
Open access · cc-by-nc-nd

Abstract

Abstract Consumers are increasingly consuming sprouted seeds due to their low calories content, nutritional value, as well as beneficial effects on human health. Sprouts contain many bioactive compounds such as minerals, fibre, vitamin C, carotenoids and phenolic compounds. The aim of this study was to determine the effects of darkness, light and time total phenolic content and scavenging activity in alfalfa (Medicago sativa), radish (Raphanus sativus), broccoli (Brassica oleracea) and hemp (Cannabis sativa) seeds during germination. The seeds were washed, soaked in water by ratio of 2:1 (water : seeds) for 12±1 h and then germinated in light and dark conditions at a temperature of 22±2 °C and a relative humidity of 85±2% for different times (12, 24, 36 and 48 hours) in a climatic chamber ICH110 (Memmert, Germany). The quality changes of germinated seeds are determined by phenol content and scavenging activity. Un-germinated seeds were used as a control. The results of current studies show that after germination the highest increase in total phenols was in radish, broccoli and alfalfa seeds. The scavenging activity (SA) was higher after 48 hours of germination in all seed types compared to un-germinated seeds both in darkness and in light. This study shows that sprouted edible seeds are an excellent source of total phenolic compounds and has a high scavenging activity.

Abstract from the paper’s OpenAlex record; open copy licensed cc-by-nc-nd. Read at the source ↗

The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.

Claims 4

Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Radish seeds showed among the highest increases in total phenols after germination.

Treatment
germinatione0
Outcome
total phenolse0
Direction
increasee0
Comparator
Un-germinated seedse1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Raphanus sativus
Raphanus sativus · TaxonSelf, en:User:Jengod · Public domain · Wikimedia Commons ↗

Verbatim

e0

“after germination the highest increase in total phenols was in radish, broccoli and alfalfa seeds”

✓ located verbatim in the source · abstract, characters 1010–1107 · show in text ↑
e1

“Un-germinated seeds were used as a control”

✓ located verbatim in the source · abstract, characters 925–967 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Broccoli seeds showed among the highest increases in total phenols after germination.

Treatment
germinatione0
Outcome
total phenolse0
Direction
increasee0
Comparator
Un-germinated seedse1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica oleracea var. italica
Brassica oleracea var. italica · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“after germination the highest increase in total phenols was in radish, broccoli and alfalfa seeds”

✓ located verbatim in the source · abstract, characters 1010–1107 · show in text ↑
e1

“Un-germinated seeds were used as a control”

✓ located verbatim in the source · abstract, characters 925–967 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Alfalfa seeds showed among the highest increases in total phenols after germination.

Taxon
alfalfaMedicago sativae0
Treatment
germinatione0
Outcome
total phenolse0
Direction
increasee0
Comparator
Un-germinated seedse1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Medicago sativa
Medicago sativa · TaxonDrepanostoma · CC BY 4.0 · iNaturalist ↗

Verbatim

e0

“after germination the highest increase in total phenols was in radish, broccoli and alfalfa seeds”

✓ located verbatim in the source · abstract, characters 1010–1107 · show in text ↑
e1

“Un-germinated seeds were used as a control”

✓ located verbatim in the source · abstract, characters 925–967 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Scavenging activity increased after 48 hours of germination in all tested seed types in light and darkness.

Taxon
alfalfa (Medicago sativa), radish (Raphanus sativus), broccoli (Brassica oleracea) and hemp (Cannabis sativa)Cannabis sativaBrassica oleraceaRaphanus sativusMedicago sativae0
Treatment
48 hours of germinatione1
Outcome
scavenging activity (SA)e1
Direction
highere1
Qualifier
both in darkness and in lighte1
Comparator
un-germinated seedse1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cannabis sativa
Cannabis sativa · TaxonThayne Tuason · CC BY-SA 4.0 · Wikimedia Commons ↗
Brassica oleracea
Brassica oleracea · TaxonDaderot · Public domain · Wikimedia Commons ↗
Raphanus sativus
Raphanus sativus · TaxonSelf, en:User:Jengod · Public domain · Wikimedia Commons ↗
Medicago sativa
Medicago sativa · TaxonDrepanostoma · CC BY 4.0 · iNaturalist ↗

Verbatim

e0

“alfalfa (Medicago sativa), radish (Raphanus sativus), broccoli (Brassica oleracea) and hemp (Cannabis sativa) seeds”

✓ located verbatim in the source · abstract, characters 404–519 · show in text ↑
e1

“The scavenging activity (SA) was higher after 48 hours of germination in all seed types compared to un-germinated seeds both in darkness and in light”

✓ located verbatim in the source · abstract, characters 1109–1258 · show in text ↑

processing · molecular: metabolites · Cannabis sativaPositive and negative results

6 papers: 5 positive, 1 negative or no effect

4 papers in the same direction as this paper · 1 in the opposite direction

  1. no effect
  2. positive
  3. positive

2 papers more in this group

See all 6 papers →

processing · molecular: metabolites · Medicago sativa (alfalfa)Positive and negative results

24 papers: 21 positive, 3 negative or no effect

20 papers in the same direction as this paper · 3 in the opposite direction

  1. no effect
  2. negative
  3. negative

20 papers more in this group

See all 24 papers →

processing · molecular: metabolites · Brassica oleracea (wild cabbage)Positive and negative results

24 papers: 18 positive, 6 negative or no effect

17 papers in the same direction as this paper · 6 in the opposite direction

  1. negative
  2. no effect
  3. negative

20 papers more in this group

See all 24 papers →

processing · quality: phytochemicals · Brassica oleracea (wild cabbage)Positive and negative results

24 papers: 12 positive, 12 negative or no effect

11 papers in the same direction as this paper · 11 in the opposite direction · 1 reporting no difference from an alternative

  1. no effect
  2. negative
  3. negative

20 papers more in this group

See all 24 papers →

processing · molecular: metabolites · Raphanus sativus (radish)Results agree

2 papers: 2 positive, 0 negative or no effect

1 paper in the same direction as this paper

  1. positive
See all 2 papers →

processing · quality: phytochemicals · Medicago sativa (alfalfa)Results agree

5 papers: 5 positive, 0 negative or no effect

4 papers in the same direction as this paper

  1. positive
  2. positive
  3. positive

1 paper more in this group

See all 5 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.