Abstract
Background: Taxol (Paclitaxel) has been highly recommended to control and treat a wide range of cancers. Taxus baccata L. is primary and natural origin of Taxol. Objective: Due to severe restriction and prohibition of cutting T. baccata, the alternative way for Taxol production is to apply in vitro culture method which was implemented at the current study. Here, for the first time, the effect of amino acid complex as elicitor along with chitosan in cell suspension culture of T. baccata for enhancing Taxol production was studied. Methods: Young leaves of T. baccata as explants were cultured in different callus induction treatments. At the next step, the calli from the best callus induction treatment were transferred to cell suspension cultures containing chitosan and amino acid complex alone or in combination with each other. Taxol content in treatments were measured by HPLC. Results: MS medium supplemented with 2,4-D at 2 mg/L and NAA at 1 mg/L proved to be the best treatment of callus induction (100 %), fresh weight (495 mg) and dry weight (272 mg) of calli. Also, HPLC analysis confirmed the maximum production of Taxol (1.96 mg/g) in MS medium having 2 ml/L amino acids complex with 10 mg/L chitosan. Conclusion: Applying amino acid complex as elicitor with chitosan is suggested for enhancing Taxol production in cell suspension culture of T. baccata.
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Claims 5
Claim · model-written summary
The specified medium produced 100% callus induction.
- Taxon
- Taxus baccata L.
Taxus baccatae1 - Treatment
- MS medium supplemented with 2,4-D at 2 mg/L and NAA at 1 mg/Le0
- Outcome
- callus inductione0
- Direction
- beste0
- Value
- 100 %e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MS medium supplemented with 2,4-D at 2 mg/L and NAA at 1 mg/L proved to be the best treatment of callus induction (100 %)”
e1“Taxus baccata L. is primary and natural origin of Taxol”
Claim · model-written summary
The specified medium produced 495 mg fresh callus weight.
- Taxon
- Taxus baccata L.Taxus baccatae1
- Treatment
- MS medium supplemented with 2,4-D at 2 mg/L and NAA at 1 mg/Le0
- Outcome
- fresh weighte0
- Direction
- beste0
- Value
- 495 mge0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MS medium supplemented with 2,4-D at 2 mg/L and NAA at 1 mg/L proved to be the best treatment of callus induction (100 %), fresh weight (495 mg)”
e1“Taxus baccata L. is primary and natural origin of Taxol”
Claim · model-written summary
The specified medium produced 272 mg dry callus weight.
- Taxon
- Taxus baccata L.Taxus baccatae1
- Treatment
- MS medium supplemented with 2,4-D at 2 mg/L and NAA at 1 mg/Le0
- Outcome
- dry weighte0
- Direction
- beste0
- Value
- 272 mge0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MS medium supplemented with 2,4-D at 2 mg/L and NAA at 1 mg/L proved to be the best treatment of callus induction (100 %), fresh weight (495 mg) and dry weight (272 mg)”
e1“Taxus baccata L. is primary and natural origin of Taxol”
Claim · model-written summary
Amino acid complex with chitosan produced the maximum Taxol level of 1.96 mg/g.
- Taxon
- Taxus baccata L.Taxus baccatae1
- Treatment
- MS medium having 2 ml/L amino acids complex with 10 mg/L chitosane0
- Outcome
- production of Taxole0
- Direction
- maximume0
- Value
- 1.96 mg/ge0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“maximum production of Taxol (1.96 mg/g) in MS medium having 2 ml/L amino acids complex with 10 mg/L chitosan”
e1“Taxus baccata L. is primary and natural origin of Taxol”
Claim · model-written summary
The authors suggest amino acid complex with chitosan to enhance Taxol production in yew cell suspension culture.
- Taxon
- T. baccataTaxus baccatae0
- Treatment
- Applying amino acid complex as elicitor with chitosane0
- Outcome
- enhancing Taxol production in cell suspension culturee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Applying amino acid complex as elicitor with chitosan is suggested for enhancing Taxol production in cell suspension culture of T. baccata”
The same questions in other papers
1-naphthaleneacetic acid · growth · Taxus baccata (English yew)Results agree
1 paper in the same direction as this paper
- positive
in vitro · growth · Taxus baccata (English yew)Results agree
3 papers in the same direction as this paper
- positive
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.