Abstract
Introduction: Plantago lanceolata is one the most important species of the Plantago genus and has valuable medicinal secondary metabolites. Materials and Methods: The effect of different factors on germination of P. lanceolata seeds was studied and leaf and root explants of in vitro growth seedling were cultured on Murashige and Skoog (MS) medium supplemented with combination of 6-benzylaminopurine (BAP) or thidiazuron (TDZ) (0, 0.5, 1, 1.5, 2 and 3 mg/L) and auxins: α-naphthaleneacetic acid (NAA) (0, 0.2, 0.5, 1 mg/L), Indole-3-acetic acid (IAA) (0.1, 0.5, 1, 1.5 mg/L) or Indole-3-butyric acid (0.5, 1, 1.5, 2 mg/L) (IBA) at different concentrations. Results: The results showed that cold pre-treatment, daylight and 1/8 MS salt concentration are more suitable for high germination. The best shoot organogenesis rate (95%) in leaf explants was observed in 1:1 mg/L, and 1.5:1 mg/L TDZ: IBA. The highest percentage of shoot organogenesis (100%) was observed in most of the plant growth regulator (PGR) treatments in root explants. About 58.67 and 60 shoot numbers obtained with 2 mg/L TDZ in leaf and 1:1.5 mg/L BAP: IBA in root explants, respectively. Conclusions: It can be suggested that the best shoot organogenesis and proliferation medium is MS basal medium containing cytokinin TDZ and auxin IBA in comparison with other hormone compounds on leaf and root explants. The result behind this fact is high callus induction and regeneration potential of explants in all concentrations.
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Claims 7
Claim · model-written summary
Cold pre-treatment, daylight and 1/8 MS salt concentration are more suitable for high germination
- Taxon
- Plantago lanceolata
Plantago lanceolatat0 - Treatment
- cold pre-treatment, daylight and 1/8 MS salt concentratione1
- Outcome
- high germinatione1
- Direction
- more suitablee1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Tissue Culture, In Vitro Organogenesis and Regeneration of Plantago lanceolata”
e1“The results showed that cold pre-treatment, daylight and 1/8 MS salt concentration are more suitable for high germination”
Claim · model-written summary
TDZ: IBA in leaf explants gave the best shoot organogenesis rate of 95%
- Taxon
- Plantago lanceolataPlantago lanceolatat0
- Organ / stage
- leaf explantse2
- Treatment
- TDZ: IBAe2
- Outcome
- shoot organogenesis ratee2
- Direction
- beste2
- Value
- 95%e2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Tissue Culture, In Vitro Organogenesis and Regeneration of Plantago lanceolata”
e2“The best shoot organogenesis rate (95%) in leaf explants was observed in 1:1 mg/L, and 1.5:1 mg/L TDZ: IBA”
Claim · model-written summary
Most plant growth regulator treatments gave the highest shoot organogenesis of 100% in root explants
- Taxon
- Plantago lanceolataPlantago lanceolatat0
- Organ / stage
- root explantse3
- Treatment
- plant growth regulator (PGR) treatmentse3
- Outcome
- shoot organogenesise3
- Direction
- highest percentagee3
- Value
- 100%e3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Tissue Culture, In Vitro Organogenesis and Regeneration of Plantago lanceolata”
e3“The highest percentage of shoot organogenesis (100%) was observed in most of the plant growth regulator (PGR) treatments in root explants”
Claim · model-written summary
Leaf explants with 2 mg/L TDZ produced 58.67 shoot numbers
- Taxon
- Plantago lanceolataPlantago lanceolatat0
- Organ / stage
- leafe4
- Treatment
- 2 mg/L TDZe4
- Outcome
- shoot numberse4
- Value
- 58.67e4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Tissue Culture, In Vitro Organogenesis and Regeneration of Plantago lanceolata”
e4“About 58.67 and 60 shoot numbers obtained with 2 mg/L TDZ in leaf and 1:1.5 mg/L BAP: IBA in root explants, respectively”
Claim · model-written summary
Root explants with 1:1.5 mg/L BAP: IBA produced 60 shoot numbers
- Taxon
- Plantago lanceolataPlantago lanceolatat0
- Organ / stage
- root explantse4
- Treatment
- BAP: IBAe4
- Outcome
- shoot numberse4
- Value
- 60e4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Tissue Culture, In Vitro Organogenesis and Regeneration of Plantago lanceolata”
e4“About 58.67 and 60 shoot numbers obtained with 2 mg/L TDZ in leaf and 1:1.5 mg/L BAP: IBA in root explants, respectively”
Claim · model-written summary
MS basal medium with TDZ and IBA is the best shoot organogenesis and proliferation medium for leaf and root explants
- Taxon
- Plantago lanceolataPlantago lanceolatat0
- Outcome
- best shoot organogenesis and proliferation mediume5
- Qualifier
- MS basal medium containing cytokinin TDZ and auxin IBAe5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Tissue Culture, In Vitro Organogenesis and Regeneration of Plantago lanceolata”
e5“It can be suggested that the best shoot organogenesis and proliferation medium is MS basal medium containing cytokinin TDZ and auxin IBA in comparison with other hormone compounds on leaf and root explants”
Claim · model-written summary
The best medium gives high callus induction and regeneration potential of explants
- Taxon
- Plantago lanceolataPlantago lanceolatat0
- Outcome
- best shoot organogenesis and proliferation mediume5
- Qualifier
- high callus inductione6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Tissue Culture, In Vitro Organogenesis and Regeneration of Plantago lanceolata”
e5“It can be suggested that the best shoot organogenesis and proliferation medium is MS basal medium containing cytokinin TDZ and auxin IBA in comparison with other hormone compounds on leaf and root explants”
e6“The result behind this fact is high callus induction and regeneration potential of explants in all concentrations”
The same questions in other papers
cold · growth · Plantago lanceolata (narrow-leaved plantain)Results agree
2 papers in the same direction as this paper
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.