Abstract
In vitro strategies for the propagation and conservation of indigenous species contribute to the sustainable use of plant diversity and are essential for breeding programs as well. In this study, we established an efficient protocol for the micropropagation of autochthonous plum 'Crvena Ranka' and examined the survival and regrowth capacity of in vitro shoots after 3, 6 and 9 months of cold storage (CS) at +5 oC in total darkness. Aseptic culture was established on the Murashige and Skoog medium containing 2 mg l-1 BA, 0.5 mg l-1 IBA and 0.1 mg l-1 GA3 (leaf rosette initiation being 68.8%). During in vitro propagation on the medium of constant hormonal composition, a significant increase in the multiplication index was observed in the third subculture, whereupon it was mainly stable until the fifth subculture. The effect of BA concentration and/or type of auxins (IBA or NAA) on multiplication parameters, as well as on fresh and dry weights of shoots was evaluated. BA at 1 mg l-1 in combination with NAA significantly increased shoot multiplication parameters. The effect of auxins on rooting parameters was monitored as well. Shoots cultured on the medium supplemented with NAA also displayed higher rooting ability (60%), in comparison with those grown on the medium containing IBA at the same concentration (20%). In vitro shoots can be conserved over the medium term under CS conditions up to six months. High survival was achieved after three (94%) and six months (82.5%), while severe signs of necrosis (100%) were noticed after nine months of conservation. Shoots subcultured under standard growth conditions after CS promptly regained their morphology although their capacity for multiplication and rooting was slightly lower than that of non-cold-stored shoots.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by-nd. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 9
Claim · model-written summary
The establishment medium yielded 68.8% leaf rosette initiation.
- Taxon
- plum
Prunus domesticat0 - Material
- 'Crvena Ranka't0
- Treatment
- 2 mg l-1 BA, 0.5 mg l-1 IBA and 0.1 mg l-1 GA3e0
- Outcome
- leaf rosette initiatione0
- Value
- 68.8%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Aseptic culture was established on the Murashige and Skoog medium containing 2 mg l-1 BA, 0.5 mg l-1 IBA and 0.1 mg l-1 GA3 (leaf rosette initiation being 68.8%).”
t0“plum cultivar 'Crvena Ranka'”
Claim · model-written summary
Multiplication index increased significantly in the third subculture and then was mainly stable to the fifth.
- Taxon
- plumPrunus domesticat0
- Material
- 'Crvena Ranka't0
- Organ / stage
- third subculturee0
- Outcome
- multiplication indexe0
- Direction
- increasee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a significant increase in the multiplication index was observed in the third subculture, whereupon it was mainly stable until the fifth subculture.”
t0“plum cultivar 'Crvena Ranka'”
Claim · model-written summary
BA with NAA significantly increased shoot multiplication parameters.
- Taxon
- plumPrunus domesticat0
- Material
- 'Crvena Ranka't0
- Treatment
- BA at 1 mg l-1 in combination with NAAe0
- Outcome
- shoot multiplication parameterse0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“BA at 1 mg l-1 in combination with NAA significantly increased shoot multiplication parameters.”
t0“plum cultivar 'Crvena Ranka'”
Claim · model-written summary
NAA medium gave 60% rooting, higher than IBA medium at 20%.
- Taxon
- plumPrunus domesticat0
- Material
- 'Crvena Ranka't0
- Treatment
- NAAe0
- Outcome
- rooting abilitye0
- Direction
- highere0
- Value
- 60%e0
- Qualifier
- 20%e0
- Comparator
- IBAe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Shoots cultured on the medium supplemented with NAA also displayed higher rooting ability (60%), in comparison with those grown on the medium containing IBA at the same concentration (20%).”
t0“plum cultivar 'Crvena Ranka'”
Claim · model-written summary
Shoot survival after three months of cold storage was 94%.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“High survival was achieved after three (94%) and six months (82.5%)”
t0“plum cultivar 'Crvena Ranka'”
Claim · model-written summary
Shoot survival after six months of cold storage was 82.5%.
- Taxon
- plumPrunus domesticat0
- Material
- 'Crvena Ranka't0
- Outcome
- survivale0
- Value
- 82.5%e0
- Qualifier
- six monthse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“High survival was achieved after three (94%) and six months (82.5%)”
t0“plum cultivar 'Crvena Ranka'”
Claim · model-written summary
All shoots showed necrosis after nine months of cold storage.
- Taxon
- plumPrunus domesticat0
- Material
- 'Crvena Ranka't0
- Outcome
- severe signs of necrosise0
- Value
- 100%e0
- Qualifier
- nine monthse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“severe signs of necrosis (100%) were noticed after nine months of conservation.”
t0“plum cultivar 'Crvena Ranka'”
Claim · model-written summary
Cold-stored shoots regained their morphology after standard-condition subculture.
- Taxon
- plumPrunus domesticat0
- Material
- 'Crvena Ranka't0
- Organ / stage
- Shootse0
- Outcome
- regained their morphologye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Shoots subcultured under standard growth conditions after CS promptly regained their morphology”
t0“plum cultivar 'Crvena Ranka'”
Claim · model-written summary
Cold-stored shoots had slightly lower multiplication and rooting capacity than non-stored shoots.
- Taxon
- plumPrunus domesticat0
- Material
- 'Crvena Ranka't0
- Treatment
- CSe1
- Outcome
- capacity for multiplication and rootinge0
- Direction
- lowere0
- Comparator
- non-cold-stored shootse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“their capacity for multiplication and rooting was slightly lower than that of non-cold-stored shoots.”
t0“plum cultivar 'Crvena Ranka'”
e1“Shoots subcultured under standard growth conditions after CS promptly regained their morphology”
The same questions in other papers
N-benzyladenine · pest · Prunus domestica (plum)Positive and negative results
1 paper in the same direction as this paper · 1 in the opposite direction
- negative
- positive
1-naphthaleneacetic acid · growth · Prunus domestica (plum)Positive and negative results
1 paper in the same direction as this paper · 2 in the opposite direction
- negative
- negative
- positive
cold · pest · Prunus domestica (plum)Positive and negative results
1 in the opposite direction
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.