Abstract
V2 proteins encoded by some whitefly-transmitted geminiviruses were reported to be functionally important proteins. However, the functions of the V2 protein of tobacco curly shoot virus (TbCSV), a monopartite begomovirus that causes leaf curl disease on tomato and tobacco in China, remains to be characterized. In our report, an Agrobacterium infiltration-mediated transient expression assay indicated that TbCSV V2 can suppress local and systemic RNA silencing and the deletion analyses demonstrated that the amino acid region 1–92 of V2, including the five predicted α-helices, are required for local RNA silencing suppression. Site-directed substitutions showed that the conserved basic and ring-structured amino acids in TbCSV V2 are critical for its suppressor activity. Potato virus X-mediated heteroexpression of TbCSV V2 in Nicotiana benthamiana induced hypersensitive response-like (HR-like) cell death and systemic necrosis in a manner independent of V2′s suppressor activity. Furthermore, TbCSV infectious clone mutant with untranslated V2 protein (TbCSV∆V2) could not induce visual symptoms, and coinfection with betasatellite (TbCSB) could obviously elevate the viral accumulation and symptom development. Interestingly, symptom recovery occurred at 15 days postinoculation (dpi) and onward in TbCSV∆V2/TbCSB-inoculated plants. The presented work contributes to understanding the RNA silencing suppression activity of TbCSV V2 and extends our knowledge of the multifunctional role of begomovirus-encoded V2 proteins during viral infections.
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Claims 9
Claim · model-written summary
TbCSV V2 suppressed local and systemic RNA silencing.
- Taxon
- tobacco
Nicotiana tabacume1 - Interactor
- TbCSVe0pathogen
- Gene
- TbCSV V2e0
- Outcome
- local and systemic RNA silencinge0
- Direction
- suppresse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“TbCSV V2 can suppress local and systemic RNA silencing”
e1“tomato and tobacco”
Claim · model-written summary
The V2 amino acid region 1–92 was required for local silencing suppression.
- Taxon
- tobaccoNicotiana tabacume1
- Gene
- V2e0
- Outcome
- local RNA silencing suppressione0
- Qualifier
- amino acid region 1–92e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the amino acid region 1–92 of V2, including the five predicted α-helices, are required for local RNA silencing suppression”
e1“tomato and tobacco”
Claim · model-written summary
Conserved basic and ring-structured V2 amino acids were critical for suppressor activity.
- Taxon
- tobaccoNicotiana tabacume1
- Gene
- TbCSV V2e0
- Outcome
- suppressor activitye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the conserved basic and ring-structured amino acids in TbCSV V2 are critical for its suppressor activity”
e1“tomato and tobacco”
Claim · model-written summary
V2 heteroexpression induced hypersensitive-like cell death in Nicotiana benthamiana.
- Taxon
- Nicotiana benthamiana
Nicotiana benthamianae0 - Treatment
- Potato virus X-mediated heteroexpression of TbCSV V2e0
- Interactor
- Potato virus XPotato virus Xe0pathogen
- Gene
- TbCSV V2e0
- Outcome
- hypersensitive response-like (HR-like) cell deathe0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Potato virus X-mediated heteroexpression of TbCSV V2 in Nicotiana benthamiana induced hypersensitive response-like (HR-like) cell death”
Claim · model-written summary
V2 heteroexpression induced systemic necrosis independently of silencing suppression.
- Taxon
- Nicotiana benthamianaNicotiana benthamianae0
- Treatment
- Potato virus X-mediated heteroexpression of TbCSV V2e0
- Interactor
- Potato virus XPotato virus Xe0pathogen
- Outcome
- systemic necrosise0
- Qualifier
- independent of V2′s suppressor activitye0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Potato virus X-mediated heteroexpression of TbCSV V2 in Nicotiana benthamiana induced hypersensitive response-like (HR-like) cell death and systemic necrosis in a manner independent of V2′s suppressor activity”
Claim · model-written summary
The V2 untranslated mutant did not induce visible symptoms.
- Taxon
- tobaccoNicotiana tabacume1
- Material
- TbCSV∆V2e0
- Interactor
- TbCSVe0pathogen
- Outcome
- could not induce visual symptomse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“TbCSV infectious clone mutant with untranslated V2 protein (TbCSV∆V2) could not induce visual symptoms”
e1“tomato and tobacco”
Claim · model-written summary
Betasatellite coinfection elevated viral accumulation.
- Taxon
- tobaccoNicotiana tabacume1
- Treatment
- coinfection with betasatellite (TbCSB)e0
- Interactor
- TbCSBe0pathogen
- Outcome
- viral accumulatione0
- Direction
- elevatee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“coinfection with betasatellite (TbCSB) could obviously elevate the viral accumulation”
e1“tomato and tobacco”
Claim · model-written summary
Betasatellite coinfection elevated symptom development.
- Taxon
- tobaccoNicotiana tabacume1
- Treatment
- coinfection with betasatellite (TbCSB)e0
- Interactor
- TbCSBe0pathogen
- Outcome
- symptom developmente0
- Direction
- elevatee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“coinfection with betasatellite (TbCSB) could obviously elevate the viral accumulation and symptom development”
e1“tomato and tobacco”
Claim · model-written summary
Symptoms recovered from 15 days postinoculation onward in mutant and betasatellite coinfected plants.
- Taxon
- tobaccoNicotiana tabacume1
- Interactor
- TbCSV∆V2e0pathogen
- Second interactor
- TbCSBe0pathogen
- Outcome
- symptom recoverye0
- Value
- 15 days postinoculation (dpi)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“symptom recovery occurred at 15 days postinoculation (dpi) and onward in TbCSV∆V2/TbCSB-inoculated plants”
e1“tomato and tobacco”
The same questions in other papers
Nicotiana tabacum · Potato virus X · Nicotiana benthamianaPositive and negative results
1 paper in the same direction as this paper · 1 in the opposite direction
- positive
- negative
Solanum tuberosum · Potato virus X · Nicotiana benthamianaPositive and negative results
22 papers in the same direction as this paper · 2 in the opposite direction
- negative
- positive
- positive
21 papers more in this group
See all 25 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.