Abstract
The Potato virus X (PVX)-based vector was used for the construction of N- and C-terminally modified PVX coat protein (XCP) chimeras. N-terminal XCP modifications do not influence the viral life cycle, whereas the simple XCP C-terminal fusion impedes the viral replication. We designed several C-terminally modified XCP chimeras and tested their viabilities in various Nicotiana benthamiana genotypes. Our results showed the negative impact of 3'-terminal modification of XCP on the chimera's life cycle. To ensure chimeric constructs stability, the second copy of the last 60 nucleotides of XCP followed by the 3'-untranslated region (UTR) was added downstream of the recombinant sequence. Simultaneously, the first copy of the last 60 nucleotides of XCP was mutated in order to prevent recombination between the two identical sequences. The movement protein of Tobacco mosaic virus expressed in transgenic N. benthamiana plants positively affected the cell-to-cell spread of C-terminally modified XCP chimeras.
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Claims 3
Claim · model-written summary
N-terminal modifications of the PVX coat protein do not influence the viral life cycle.
- Taxon
- Nicotiana benthamiana
Nicotiana benthamianae4 - Treatment
- N-terminal XCP modificationse1
- Interactor
- Potato virus XPotato virus Xe0pathogen
- Outcome
- viral life cyclee1
- Direction
- do not influencee1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“N-terminal XCP modifications do not influence the viral life cycle”
e0“The Potato virus X (PVX)-based vector”
e4“various Nicotiana benthamiana genotypes”
Claim · model-written summary
The simple C-terminal fusion of the PVX coat protein impedes viral replication.
- Taxon
- Nicotiana benthamianaNicotiana benthamianae4
- Treatment
- simple XCP C-terminal fusione2
- Interactor
- Potato virus XPotato virus Xe0pathogen
- Outcome
- viral replicatione2
- Direction
- impedese2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“the simple XCP C-terminal fusion impedes the viral replication”
e0“The Potato virus X (PVX)-based vector”
e4“various Nicotiana benthamiana genotypes”
Claim · model-written summary
Movement protein of Tobacco mosaic virus positively affected cell-to-cell spread of C-terminally modified PVX coat protein chimeras.
- Taxon
- N. benthamianaNicotiana benthamianae3
- Treatment
- movement protein of Tobacco mosaic virus expressed in transgenic N. benthamiana plantse3
- Interactor
- Potato virus XPotato virus Xe0pathogen
- Second interactor
- Tobacco mosaic virus
Tobacco mosaic viruse3other - Outcome
- cell-to-cell spreade3
- Direction
- positively affectede3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e3“The movement protein of Tobacco mosaic virus expressed in transgenic N. benthamiana plants positively affected the cell-to-cell spread of C-terminally modified XCP chimeras”
e0“The Potato virus X (PVX)-based vector”
The same questions in other papers
Nicotiana tabacum · Potato virus X · Nicotiana benthamianaPositive and negative results
2 in the opposite direction
- negative
- negative
transgenic · Potato virus X · Nicotiana benthamianaPositive and negative results
1 paper in the same direction as this paper · 12 in the opposite direction
- negative
- negative
- negative
10 papers more in this group
See all 14 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.