Abstract
Existing Cannabis sativa micropropagation protocols use a limited number of cultivars and the results are often not reproducible. Currently, Murashige and Skoog basal salt mixture (MS) + 0.5 μmol/L thidiazuron (TDZ) has been reported as the optimal medium for nodal micropropagation, yet our preliminary studies with this medium have resulted in abnormal morphology and high mortality rates in multiple cultivars. Following an initial screen of basal salt mixtures [MS, B5, BABI, and Driver–Kuniyaki–Walnut (DKW)], we determined that DKW produced the healthiest plants. In a second experiment, the multiplication rate and canopy area of explants grown on MS + 0.5 μmol/L TDZ and DKW + 0.5 μmol/L TDZ were compared using five drug-type cultivars. The combined multiplication average of explants grown on DKW + 0.5 μmol/L TDZ was 1.5× higher than explants grown on MS + 0.5 μmol/L TDZ. Similarly, the combined average of the canopy area was twice as large on DKW + 0.5 μmol/L TDZ. In the third experiment, callogenesis was compared using a concentration range of 2,4-dichlorophenoxyacetic acid (0–30 μmol/L) on both MS and DKW, and similarly, callus growth was superior on DKW. This study presents the largest comparison of basal salt compositions on the micropropagation of five commercially grown Cannabis cultivars to date.
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Claims 5
Claim · model-written summary
MS plus TDZ caused abnormal morphology and high mortality in multiple cultivars.
- Taxon
- Cannabis sativa
Cannabis sativat0 - Treatment
- this mediume0
- Outcome
- abnormal morphology and high mortality ratese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“our preliminary studies with this medium have resulted in abnormal morphology and high mortality rates in multiple cultivars”
t0“Cannabis sativa”
Claim · model-written summary
DKW produced the healthiest plants in the initial basal salt screen.
- Taxon
- Cannabis sativaCannabis sativat0
- Treatment
- DKWe0
- Outcome
- healthiest plantse0
- Direction
- healthieste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we determined that DKW produced the healthiest plants”
t0“Cannabis sativa”
Claim · model-written summary
DKW plus TDZ gave 1.5 times the multiplication average of MS plus TDZ.
- Taxon
- Cannabis sativaCannabis sativat0
- Treatment
- DKW + 0.5 μmol/L TDZe0
- Outcome
- multiplication averagee0
- Direction
- highere0
- Value
- 1.5×e0
- Comparator
- MS + 0.5 μmol/L TDZe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The combined multiplication average of explants grown on DKW + 0.5 μmol/L TDZ was 1.5× higher than explants grown on MS + 0.5 μmol/L TDZ”
t0“Cannabis sativa”
Claim · model-written summary
DKW plus TDZ doubled average canopy area relative to MS plus TDZ.
- Taxon
- Cannabis sativaCannabis sativat0
- Treatment
- DKW + 0.5 μmol/L TDZe0
- Outcome
- canopy areae0
- Direction
- twice as largee0
- Comparator
- MS + 0.5 μmol/L TDZe1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the combined average of the canopy area was twice as large on DKW + 0.5 μmol/L TDZ”
t0“Cannabis sativa”
e1“The combined multiplication average of explants grown on DKW + 0.5 μmol/L TDZ was 1.5× higher than explants grown on MS + 0.5 μmol/L TDZ”
Claim · model-written summary
Callus growth was superior on DKW.
- Taxon
- Cannabis sativaCannabis sativat0
- Treatment
- DKWe0
- Outcome
- callus growthe0
- Direction
- superiore0
- Comparator
- MSt1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“callus growth was superior on DKW”
t0“Cannabis sativa”
t1“MS basal salts”
The same questions in other papers
Thidiazuron · growth · Cannabis sativaPositive and negative results
10 papers in the same direction as this paper · 1 in the opposite direction
- negative
- positive
- positive
8 papers more in this group
See all 12 papers →Thidiazuron · pest · Cannabis sativaResults agree
2 papers in the same direction as this paper
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.