Abstract
The present study was carried out to investigate micropropagation possibility and determine the optimal medium composition and plant growth regulators (PGRs) combinations under in vitro conditions. The cotyledon and epicotyl explants obtained from 1 month old in vitro grown seedlings were used in MS medium containing BA (0.1, 0.2, 0.5, 1, 2 and 3 mg-1) and TDZ (0.1, 0.2, 0.5, 1, 2 and 3 mg-1) either alone or in combination with 0.5 mg-1 IBA. The response of cannabis explants to PGRs treatments was much different from those observed in most of plant species. That is, callus formation had priority over direct regeneration in most of the PGRs treatments. Comparing the two explants, cotyledon had higher callus formation frequency and the largest callus volume was obtained for this explant in MS medium supplemented with 3 mg-1 TDZ + 0.5 mg-1 IBA. The highest callus fresh weight (3.15 gr) was obtained for cotyledon explant treated with 2 mg-1 TDZ+ 0.5 mg-1 IBA. In shoot formation step, the highest rate of shoot regeneration was achieved in the calli produced from epicotyl explant treated with 2 mg-1 BA + 0.5 mg-1 IBA; and the highest length of regenerated shoots (1.23 cm) was observed in 2 mg-1 BA + 0.5 mg-1 IBA treatment. In general, cotyledon was the best explant and TDZ in combination with IBA was the best treatment for callus formation. Epicotyl explant also showed better regeneration compared to cotyledon.
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Claims 9
Claim · model-written summary
Callus formation predominated over direct regeneration in most growth regulator treatments.
- Taxon
- Cannabis sativa
Cannabis sativat0 - Treatment
- PGRs treatmentse0
- Outcome
- callus formation had priority over direct regeneratione0
- Qualifier
- most of the PGRs treatmentse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“callus formation had priority over direct regeneration in most of the PGRs treatments”
t0“Cannabis sativa”
Claim · model-written summary
Cotyledon explants had higher callus formation frequency than epicotyl explants.
- Taxon
- Cannabis sativaCannabis sativat0
- Organ / stage
- cotyledone0
- Outcome
- callus formation frequencye0
- Direction
- highere0
- Comparator
- two explantse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“cotyledon had higher callus formation frequency”
e1“Comparing the two explants”
t0“Cannabis sativa”
Claim · model-written summary
Cotyledon explants produced the largest callus volume with 3 mg-1 TDZ plus 0.5 mg-1 IBA.
- Taxon
- Cannabis sativaCannabis sativat0
- Organ / stage
- cotyledone1
- Treatment
- 3 mg-1 TDZ + 0.5 mg-1 IBAe0
- Outcome
- callus volumee0
- Direction
- largeste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the largest callus volume was obtained for this explant in MS medium supplemented with 3 mg-1 TDZ + 0.5 mg-1 IBA”
e1“cotyledon had higher callus formation frequency”
t0“Cannabis sativa”
Claim · model-written summary
Cotyledon callus fresh weight peaked at 3.15 gr with 2 mg-1 TDZ plus 0.5 mg-1 IBA.
- Taxon
- Cannabis sativaCannabis sativat0
- Organ / stage
- cotyledone0
- Treatment
- 2 mg-1 TDZ+ 0.5 mg-1 IBAe0
- Outcome
- callus fresh weighte0
- Direction
- higheste0
- Value
- 3.15 gre0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The highest callus fresh weight (3.15 gr) was obtained for cotyledon explant treated with 2 mg-1 TDZ+ 0.5 mg-1 IBA”
t0“Cannabis sativa”
Claim · model-written summary
Calli from epicotyl explants treated with 2 mg-1 BA plus 0.5 mg-1 IBA had the highest shoot regeneration rate.
- Taxon
- Cannabis sativaCannabis sativat0
- Organ / stage
- epicotyle0
- Treatment
- 2 mg-1 BA + 0.5 mg-1 IBAe0
- Outcome
- rate of shoot regeneratione0
- Direction
- higheste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the highest rate of shoot regeneration was achieved in the calli produced from epicotyl explant treated with 2 mg-1 BA + 0.5 mg-1 IBA”
t0“Cannabis sativa”
Claim · model-written summary
The longest regenerated shoots measured 1.23 cm under 2 mg-1 BA plus 0.5 mg-1 IBA.
- Taxon
- Cannabis sativaCannabis sativat0
- Treatment
- 2 mg-1 BA + 0.5 mg-1 IBAe0
- Outcome
- length of regenerated shootse0
- Direction
- higheste0
- Value
- 1.23 cme0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the highest length of regenerated shoots (1.23 cm) was observed in 2 mg-1 BA + 0.5 mg-1 IBA treatment”
t0“Cannabis sativa”
Claim · model-written summary
Cotyledon was the best explant for callus formation.
- Taxon
- Cannabis sativaCannabis sativat0
- Organ / stage
- cotyledone0
- Outcome
- callus formatione1
- Direction
- beste0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“cotyledon was the best explant”
e1“for callus formation”
t0“Cannabis sativa”
Claim · model-written summary
TDZ in combination with IBA was the best treatment for callus formation.
- Taxon
- Cannabis sativaCannabis sativat0
- Treatment
- TDZ in combination with IBAe0
- Outcome
- callus formatione0
- Direction
- beste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“TDZ in combination with IBA was the best treatment for callus formation”
t0“Cannabis sativa”
Claim · model-written summary
Epicotyl explants regenerated better than cotyledon explants.
- Taxon
- Cannabis sativaCannabis sativat0
- Organ / stage
- Epicotyle0
- Outcome
- regeneratione0
- Direction
- bettere0
- Comparator
- cotyledone0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Epicotyl explant also showed better regeneration compared to cotyledon.”
t0“Cannabis sativa”
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