Abstract
Abstract The purpose was to induce and treat hemp seeds and callus, compare the methods to increase the germination rate of hemp seed, obtain suitable disinfectant for hemp seed, and different hormone ratios to promote callus growth. GA 3 , 6-BA, and IAA were used to induce hemp seed germination, and the effects of different hormones and different concentrations on hemp seed germination were studied. Tissue culture was used to induce hemp seeds to select suitable hemp seeds disinfectant and time; callus was induced by using hemp aseptic seedling leaves. GA 3 with a concentration of 20-80mg/L and IAA with a concentration of 20-40mg/L can promote the germination of hemp seeds; the best disinfection method of hemp seeds is: 70% ethanol for 1min + 10% NaClO for 18min; the most the combinations beneficial to callus growth were: 2.0mg/L 6-BA+0.5mg/L NAA. GA 3 and low-concentration IAA can promote the germination of hemp seeds; the combination of 70% ethanol and 10% sodium hypochlorite has the best disinfection effect on hemp seeds; 6-BA+NAA can obtain good quality hemp seeds hemp callus.
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Claims 4
Claim · model-written summary
GA3 at 20-80 mg/L promoted germination of hemp seeds.
- Taxon
- hemp
Cannabis sativa subsp. sativat0 - Treatment
- GA 3 with a concentration of 20-80mg/Le1
- Outcome
- germinatione1
- Direction
- promotee1
- Value
- 20-80mg/Le1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Effects of different hormones on germination and callus induction of hemp seeds”
e1“GA 3 with a concentration of 20-80mg/L and IAA with a concentration of 20-40mg/L can promote the germination of hemp seeds”
Claim · model-written summary
IAA at low concentration (20-40 mg/L) promoted germination of hemp seeds.
- Taxon
- hempCannabis sativa subsp. sativat0
- Treatment
- IAA with a concentration of 20-40mg/Le1
- Outcome
- germinatione1
- Direction
- promotee1
- Value
- 20-40mg/Le1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Effects of different hormones on germination and callus induction of hemp seeds”
e1“GA 3 with a concentration of 20-80mg/L and IAA with a concentration of 20-40mg/L can promote the germination of hemp seeds”
Claim · model-written summary
The 70% ethanol for 1 min plus 10% NaClO for 18 min treatment was the best disinfection method for hemp seeds.
- Taxon
- hempCannabis sativa subsp. sativat0
- Treatment
- 70% ethanol for 1min + 10% NaClO for 18mine2
- Outcome
- best disinfection method of hemp seedse2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Effects of different hormones on germination and callus induction of hemp seeds”
e2“the best disinfection method of hemp seeds is: 70% ethanol for 1min + 10% NaClO for 18min”
Claim · model-written summary
The combination of 2.0 mg/L 6-BA and 0.5 mg/L NAA was the most beneficial to hemp callus growth.
- Taxon
- hempCannabis sativa subsp. sativat0
- Treatment
- 2.0mg/L 6-BA+0.5mg/L NAAe3
- Outcome
- callus growthe3
- Direction
- beneficiale3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Effects of different hormones on germination and callus induction of hemp seeds”
e3“the most the combinations beneficial to callus growth were: 2.0mg/L 6-BA+0.5mg/L NAA”
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