Abstract
In vitro plant cultures of Cannabis sativa may have a valuable contribution to the production of prospective genotypes as high-quality propagation material for further breeding work towards new applications of hemp in fields of economy and medicine. The key to this study is to optimize the micropropagation process of the selected Russian variety K290 C. sativa, which has the appropriate level of cannabinoids. The aim of the present work was to comparative phenotypic study in terms of assessing the effect of the type of medium with different mineral salt content, i.e. Schenk-Hildebrandt medium (SH) and Gamborg medium (B-5), addition of vitamins and IAA auxin on growth of shoot with leaves during induction and development of adventitious roots. In total, 12 variants were compared, taking into account the presence of vitamins and two concentrations of auxin IAA (0.5 and 1.0 mg/L). Nodal and apical fragments of plants obtained from seeds were selected as explants. The experiment was performed in triplicate. Our results show that in terms of shoot length and number of leaves, the SH with the addition of IAA at a concentration of 1.0 mg/L without vitamins is the most effective. It was also found that IAA at a concentration of 0.5 mg/L in SH stimulated the formation of adventitious shoots (n = 3.21 after 3 weeks). The average number of roots was 4.65 per shoot on the SH with 0.5 mg/L. Therefore, it can be concluded that a higher content of potassium ions as well as cobalt, copper, magnesium, molybdenum ions and a lower content of calcium, zinc, boron, manganese and iron ions in the SH medium positively influenced the development of shoots and leaves. In addition, it was found that after 6 weeks, the number of leaves and their size had doubled.
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Claims 5
Claim · model-written summary
SH medium with 1.0 mg/L IAA and no vitamins was most effective for shoot length and leaf number.
- Taxon
- Cannabis sativa
Cannabis sativae0 - Treatment
- the SH with the addition of IAA at a concentration of 1.0 mg/L without vitaminse1
- Outcome
- shoot length and number of leavese1
- Direction
- most effectivee1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In vitro plant cultures of Cannabis sativa may have a valuable contribution to the production of prospective genotypes”
e1“Our results show that in terms of shoot length and number of leaves, the SH with the addition of IAA at a concentration of 1.0 mg/L without vitamins is the most effective”
Claim · model-written summary
IAA at 0.5 mg/L in SH stimulated formation of adventitious shoots.
- Taxon
- Cannabis sativaCannabis sativae0
- Treatment
- IAA at a concentration of 0.5 mg/L in SHe2
- Outcome
- formation of adventitious shootse2
- Direction
- stimulatede2
- Value
- n = 3.21e2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In vitro plant cultures of Cannabis sativa may have a valuable contribution to the production of prospective genotypes”
e2“It was also found that IAA at a concentration of 0.5 mg/L in SH stimulated the formation of adventitious shoots (n = 3.21 after 3 weeks)”
Claim · model-written summary
Average number of roots per shoot was 4.65 on SH with 0.5 mg/L IAA.
- Taxon
- Cannabis sativaCannabis sativae0
- Outcome
- average number of rootse3
- Value
- 4.65 per shoote3
- Qualifier
- on the SH with 0.5 mg/Le3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In vitro plant cultures of Cannabis sativa may have a valuable contribution to the production of prospective genotypes”
e3“The average number of roots was 4.65 per shoot on the SH with 0.5 mg/L”
Claim · model-written summary
Higher potassium, cobalt, copper, magnesium and molybdenum ions and lower calcium, zinc, boron, manganese and iron ions in SH positively influenced shoot and leaf development.
- Taxon
- Cannabis sativaCannabis sativae0
- Compound
- potassium ionse4
- Outcome
- development of shoots and leavese4
- Qualifier
- a higher content of potassium ions as well as cobalt, copper, magnesium, molybdenum ions and a lower content of calcium, zinc, boron, manganese and iron ions in the SH mediume4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In vitro plant cultures of Cannabis sativa may have a valuable contribution to the production of prospective genotypes”
e4“Therefore, it can be concluded that a higher content of potassium ions as well as cobalt, copper, magnesium, molybdenum ions and a lower content of calcium, zinc, boron, manganese and iron ions in the SH medium positively influenced the development of shoots and leaves”
Claim · model-written summary
After 6 weeks the number of leaves and their size had doubled.
- Taxon
- Cannabis sativaCannabis sativae0
- Outcome
- the number of leaves and their sizee5
- Qualifier
- after 6 weekse5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In vitro plant cultures of Cannabis sativa may have a valuable contribution to the production of prospective genotypes”
e5“In addition, it was found that after 6 weeks, the number of leaves and their size had doubled”
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